Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv.viciae

• Autorzy: Izmailov S. F. , Zhiznevskaya G.Y. , Kosenko L.V. , Troitskaya G.N. , Kudryavtseva N.N. , Borodenko L.I. , Dubrovo P.N. , Russa R. , Pietras H. , Lorkiewicz Z.
• Języki publikacji: EN

Abstrakty

EN

Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.

Słowa kluczowe

EN

lipopolysaccharide; chemical composition; fatty acid; Rhizobium leguminosarum bv.viciae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 4
• Opis fizyczny: p.1001-1009,fig.

Twórcy

autor

Izmailov S. F.

• Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

autor

Zhiznevskaya G.Y.

autor

Kosenko L.V.

autor

Troitskaya G.N.

autor

Kudryavtseva N.N.

autor

Borodenko L.I.

autor

Dubrovo P.N.

autor

Russa R.

autor

Pietras H.

autor

Lorkiewicz Z.

Bibliografia

• Abe, M., Sherwood, J.E., Hollingsworth, R.J. & Dazzo, F.B. (1984) Stimulation of clover root hair infection by lectin-binding oligosaccha­rides from capsular and extracellular polysac­charides of Rhizobium trifolii. J. Bacteriol 160, 517-520.
• Agibetov, K.A., Genina, O.S., Dubrovo, P.N., Livanova, G.J., Zhiznevskaya, G.Ya. & Izmailov, S.F. (1991) Nodulins, bacteroids and their intracellular localization in root nodules of yellow lupine. Sov. Plant Physiol 38, 71-79 (English edition).
• Andreeva, I.N., Kozlova, G.I., Livanova, G.I., Zhiznevskaya, G.Ya. & Izmailov, S.F. (1989) Electron microscope study of the peri- bacteroid space in legume root nodules. Sov. Plant Physiol 36,447-459 (English edition).
• Bassarab, S., Schenk, S.U. & Werner, D. (1989) Fatty acid composition of the peribacteroid membrane and the ER in nodules of Glycine max varies after infection by different strains of the microsymbiont Bradyrhizobium japo- nicum. Botanica Acta 102, 196-201.
• Borthakur, D., Barbur, C.E., Lamb, J.W., Daniels, M.J. & Downie, J.A. (1986) A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium legumino- sarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas. MoL Gen. Genet. 203. 320-323.
• Bradley, D.J., Butcher, G.W. & Galfre, G. (1986) Physical association between the peribacte­roid membrane and lipopolysaccharide from the bacteroid outer membrane in Rhizo-6mmrinfected pea root nodule cells. J. Cell ScL 85, 47-61.
• Carlson, R.W., Saunders, R.E., Napoli, C. & Albersheim, R (1978) Host-symbiont interac­tions. 111. Purification and partial character­ization of Rhizobium lipopolysaccharides. Plant Physiol. 62, 912-917.
• Carlson, R.W., Kalembasa, S., Turowski, D., Pachori, P. & Noel, K.D. (1987) Characteriza­tion of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development. J. Bacterial. 169, 4923-4928.
• Carlson, R.W., Bhat, U.R. & Reus, B. (1992) Rhizobium lipopolysaccharides: Their struc­ture and evidence for their importance in the nitrogen-fixing symbiotic infection of their host legumes; in Plant Biotechnology and De­velopment, pp. 33-44, CRC Press, Boca Raton.
• Carrion, M., Bhat, R.U., Reus, B. & Carlson, R.W. (1990) Isolation and characterization of the lipopolysaccharides from Bradyrhizobium japonicum. J. Bacteriol. 172, 1725-1731.
• Dische, Z. (1947) A new specific color reaction of hexuronic acid. J. Biol Chem. 167, 189.
• De Maagd, R.A., de Rijk, R., Mulders, J.H.M. & Lugtenberg, B.J.J. (1989) Immunological characterization of Rhizobium leguminosarum outer membrane antigens by use of polyclonal and monoclonal antibodies. J. Bacteriol. 171, 1136-1142.
• Entlicher, G., Kostir, J.V. & Kocourek, J. (1970) Studies on phytohemagglutinins. 111. Isola­tion and characterization of hemagglutinins from the pea (Pisum sativum L.) Biochim Biophys. Acta 221, 272-281.
• Freiberg, C., Fellay, R., Bairock, A., Broughton, W.F., Rosenthal, A. & Perrot, X. (1997) Molec­ular basis of symbiosis between Rhizobium and legumes. Nature 387, 394-401.
• Genina, O.S., Borodenko, L.I., Fedorova, E.E., Agibetov, K.A., Zhiznevskaya, G.Ya. & Izmailov, S.F. (1993) The fatty acid composi­tion of the peribacteroid membrane, the root nodule bacteroids and the plasmalemma of un­infected yellow lupin roots. Sou. Plant Physiol 40, 79-83.
• Genina, O.S., Radukina, N.L., Agibetov, K.A., Zhiznevskaya, G.Ya., Vereschagin, A.G. & Izmailov, S.F. (1990) The fatty acid composi­tion of the peribacteroid membrane and of the peribacteroid space material from Lupinus luteus L. root nodules. 8th Intern. Congress on Nitrogen Fixation. Knoxville, Tennessee, U.S.A., 1990, Abstracts G-06.
• Geremia, R.A., Cavaignas, S., Zorrequieta, A., Tor, N., Olivares, J. & Ugalde, R.A. (1987) A Rhizobium meliloti mutant that forms ineffec­tive pseudonodules in alfalfa produces exo- polysaccharide but fails to form /?-{l-2)-glucan. J. Bacteriol. 169, 880-884.
• Hancock, R.E. (1991) Bacterial outer membranes, evolving concepts. Am. Soc. Microbiol. News 57, 175-182.
• Hardy, R.W.F., Holsten, R.D., Jackson, E.K. & Burns, R.C. (1968) The acetylene-ethylene as­say for N^fixation: Laboratory and field evalu­ation. Plant Physiol. 43, 1185-1207.
• Izmailov, S.F. (1996) Physiology of symbiotic rela­tions in legume nodules: Biogenesis and func­tions of the peribacteroid membrane. Russian J. Plant Physiol 43, 672-687 (English edi­tion).
• Izmailov, S.F., Radukina, N.L., Agibetov, K.A., Kadyrov, R.M., Andreeva, I.N., Fedorova, E.E. & Zhiznevskaya, G.Ya. (1989) Peribacteroid space of the yellow lupin nodule: Isolation, its content and inactness of the peribacteroid membrane. Sov. Plant Physiol 36, 257 (Eng­lish edition).
• Kannenberg, E.L., Perotto, S., Blanciotto, V., Rathbun, E.A. & Brewin, N.J. (1994) Lipopoly­saccharide epitope expression of Rhizobium bacteroids as revealed by in situ immuno- labelling of pea root nodule section. J. Bac­teriol. 176, 2021-2032.
• Kudryavtseva, N.N., Tsydendambaev, V.D., Dubrovo, P.N., Krylova, V.V., Borodenko, L.I., Zhiznevskaya, G.Ya. & Izmailov, S.F. (1995) Role of the peribacteroid membrane in the in­teraction of the symbiotic partners in the ni­trogen-fixing root nodules of Lupinus luteus L.; in Nitrogen Fixation: Fundamentals and Appli­cations (Tikhonovich, I.A., et alf eds.) pp. 551-556, Kluwer Acad. Publ., Netherlands.
• Lorkiewicz. Z., Deryło, M., Głowacka, M., Kowalczuk, E., Russa, R. & Skorupska, A. (1981) Plasmids controlling symbiotic proper­ties; in Current Perspectives in Nitrogen Fixa­tion (Gibson, A.H. & Newton, W.E., eds.) pp. 409, Australian Academy of Sciences, Can­berra.
• Noel, K.D., Vandenbosch, K.A. & Kulpaca, B. (1986) Mutations in Rhizobium phaseoli that lead to arrested development of infection threads .J. Bacterial. 168, 1392-1401.
• Noel, K.D. (1992) Rhizobial polysaccharides re­quired in symbiosis with legumes; in Molecu­lar Signals in Plant Microbe Communication (Verma, D.P.S., ed.) pp. 341-357, CRC Press, Inc. Boca Ratoa
• Osborn, M.J. (1963) Studies on the Gram-negative cell wall, I. Evidence for the role of 2-keto- 3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium. Proc. Natl Acad. Set U.S.A. 50, 499-506.
• Oke, V. & Long, S.R. (1999) Bacterial genes in­duced within the nodule during the Rhizobium - legume symbiosis. Molec. Microbiol 32, 837-849.
• Planque, K. & Kijne, J.W. (1977) Binding of pea lectin to a glucan-type polysaccharide in the cell walls of Rhizobium leguminosarum. FEBS Lett. 73, 64-66.
• van Rhijn, P., Goldberg, R.G. & Hirsch, A.M. (1998) Lotus corniculatus nodulation specific­ity is changed by the presence of a soybean lectin gene. Plant Cell 10, 1233-1249.
• Russa, R., Bruneteau, M., Shashkov, A.S., Urbanik-Sypniewska, T. & Mayer, H. (1996) Characterization of the lipopolysaccharides from Rhizobium meliloti strain 102F51 and its nonnodulating mutant WL 113. Arch. Micro­biol 165, 26-33.
• Vasilieva, A.V. & Zhiznevskaya, G.Ya. (1988) Ni­trogen-fixing activity in broad bean root nod­ules in ontogeny. Sov. Plant Physiol (Moscow) 35, 386-392 (English edition).
• Westphal, O. & Jann, K. (1965) Bacterial lipo­polysaccharides. Extraction with phenol- wa­ter and further application of procedure. Methods Carbohydr. Chem. 5, 83-91.
• Zevenhuizen, L.P.T.M. (1981) Cellular glycogen, /?-l,2-glucans, polyhydroxybutyric acid and extracellular polysaccharides in fast-growing species of Rhizobium. Antonie van Leeuwen- hoek J. Microbiol Serol. 47, 481-497.
• Zhiznevskaya, G.Ya., Troitskaya, G.N., Dubrovo, P.N. & Kosenko, L.V. (1997) Nitrogen-defi­ciency stress in Vicia faba L., resulting from in­effective symbiosis. Russian J. Plant Physiol 44, 585-591.
• Zhiznevskaya, G.Ya., Klasens, V., Troitskaya, G. N., Borodenko, L.I., Kudryavtseva, N.N., Bobylev, G. & Sorokin, E.M. (1995) The effect of Rhizobium leguminosarum, strains on the growth of Vicia faba plants and the properties of root nodule symbiosis, 2nd Europ. Conf. on Grain Legumes, Copenhagen, Proceedings, pp.70-71.