Wplyw liofilizacji i przechowywania na przezywalnosc szczepu B-38 wirusa choroby Derzsyego

• Autorzy: Samorek-Salamonowicz E , Czekaj H.
• Języki publikacji: PL

Abstrakty

EN

Vaccine B-38 strain of Derzsy’s disease virus (DDV) was used in the study. The virus was lyophilized after the multiplication in the culture of GEF. Sucrose — glutamine, gelatine — sucrose, peptone — lactose and SPGA solutions were applied as stabilizators. The lyophilizates were kept at 4°C for 24 months. The concentrations of the virus in the samples were estimated periodically. It was found that peptone — lactose stabilizator was the most effective protector of DDV from inactivation. The virus was dissolved in Eagle’s fluid, PBS and commercial solvent. The dissolved vaccine was kept at 4°C, 21°C and —20°C for 24 h. The titre of the vaccine was determined immediately after the dissolving and then after 2, 4, 6 and 24 h. Storage of the vaccine at 21° or 4°C for more than 2 h resulted in a significant drop of its titre. Eagle’s fluid used as a disolvent did not differ significantly from the commercial solvent.

Słowa kluczowe

PL

stabilizator peptonowo-laktozowy; przechowywanie; wirus choroby Derzsy'ego; przezywalnosc; choroba Derzsy'ego; liofilizacja; choroby zwierzat; szczepionki

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1993
• Tom: 49
• Numer: 04
• Opis fizyczny: s.162-163,tab.,bibliogr.

Twórcy

autor

Samorek-Salamonowicz E

• Instytut Weterynarii, Al.Partyzantow 57, 24-100 Pulawy

autor

Czekaj H.

Bibliografia

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• 4. Górski J.: Pol. Arch. Wet. 16, 121, 1973.
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• 6. Kisary J.: Avian Pathol. 6, 327, 1977.