In vitro influence of methisoprinol on lymphocyte proliferation in dogs

• Autorzy: Siwicki A K , Mizak B.
• Języki publikacji: EN

Abstrakty

EN

The application of immunostimulators in dogs offers a novel approach to induce or modulate the mechanisms of protection against infectious diseases. In the present studies we evaluated in vitro influence of different concentrations of synthetic product - methisoprinol on the proliferative response of blood lymphocytes stimulated by pokeweed mitogen (PWM), concanavaline A and lipopolisaccharide (LPS) in dogs. The concentrations of methisoprinol between 1 to 50 μg/ml increased the mitogen-induced proliferation rate of canine T and B lymphocytes. Enhanced response to mitogens was observed both in 1-2 and 6-8 years old dogs, but the best results were observed in older animals. The results suggested that methisoprinol demonstrated a stronger effect on the lymphocytes isolated from older animals with the lower proliferative responses.

Słowa kluczowe

EN

methisoprinol; proliferation; viral infection; lymphocyte; mitogen; dog; infectious disease; in vitro

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 2
• Opis fizyczny: p.227-233,fig.

Twórcy

autor

Siwicki A K

• University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Mizak B.

Bibliografia

• 1. Blecha F.: Immunomodulation: a mean of disease prevention in stressed livestock. J. Anim. Scien., 1988, 66, 2084-2090.
• 2. Campo M., Chiavaro I., Canfarotta F.: Effect of levamisole and methisoprinol on in vitro lymphocyte reactivity in chronically irradiated subjects and patients affected by neoplasias. J. Immunopharmacology, 1982, 4, 127-137.
• 3. Campoli-Richards D.M., Sorkin E.M., Heel E.: Inosine pranobex - a preliminary review of its pharmacodynamic and pharmacokinetic properties and therapeutic efficacy. Drugs, 1986, 32, 383-424.
• 4. Dworecka-Kaszak B., Siwicki A.K., Budrewicz B.: Immunomodulation by Malassezia pachydermatis in vitro on fish model. Polish J. Vet. Sci., 1999, 2, 159-163.
• 5. Fudenberg H.H., Whitten H.D.: Immunostimulation: synthetic and biological modulators of immunity. A. Rev. Pharmacol. Toxicol., 1984, 24, 147-174.
• 6. Ginsberg T., Hadden J.W.: Immunopharmacology of methisoprinol. In: Fudenberg H.H. and Whitten H.D. (Eds). Immunomodulation: New Frontiers and Advances. Plenum Press, New York, 1984, pp. 331-348.
• 7. Greeley E.H., Kealy R.D., Ballam J.M., Lawer D.F., Segre M.: The influence of age on the canine immune system. Vet. Immunol. Immunopathol., 1996, 55, 1-10.
• 8. Hadden J.W., Wybran J.: Immunopotentiators: Isoprinosine, NPT 15392 and azimexone: modulators of lymphocyte and macrophage development and function. Adv. Immunopharmacol., 1981, 35, 457-468.
• 9. Hennesy K.J.: Isoprinosine and levamisole immunomodulation in artificially reared neonatal pigs. Am. J. Vet. Res., 1987, 48, 477-480.
• 10. Macy D.W.: Vaccine adjuvants. Sem. Med. Vet. Surg. (Small Animals) 1997, 12, 201-211.
• 11. Mosmann T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 1983, 65, 55-63.
• 12. Murayama T.S., Matsuume-Sasakai B., Furukawa T., Rinaldo C.R.: Biological response modifiers enhance the activity of natural killer cells against human cytomegalovirus-infected cells. J. Med. Virol., 1989, 29, 102-108.
• 13. Nakamura T., Miyasaka N., Pope R.M., Talal N., Russell I.J.: Immunomodulation by isoprinosine: effects on in vitro immune functions of lymphocytes from humans with autoimmune diseases. Clin. Exp. Immunol., 1983, 52, 67-74.
• 14. Nolte I.: The therapy of chronic diseases of the respiratory tract in dogs and cats. Tierärztl. Praxis, 1992, 20, 301-305.
• 15. Portoles A., Ojeda G., Rojo J.M., Pilar M.: Modulatory effect of isoprinosine on lymphocyte proliferative response under immunodepressive conditions. J. Immunopharmacology, 1983, 5, 245-256.
• 16. Siwicki A.K., Krzyżanowski J., Bartoszcze M., Mizak Z., Paluch S., Szmigielski S., Jeljaszewicz J., Pulverer G.: Adjuvant properties of killed Propionibacterium avidum KP-40 in vaccination of dogs against canine parvovirosis. Dtsch. Tierärztl. Wschr., 1998, 105, 186-190.
• 17. Siwicki A.K. Morand M., Klein P., Studnicka M., Terech-Majewska E.: Modulation of nonspecific defence mechanisms and protection against diseases in fish. Acta Vet. Brno, 1998, 67, 323-328.
• 18. Siwicki A.K., Platt-Samoraj A.: Immunomodulacja u psów - aktualny stan wiedzy. Mag. Wet. Suplement Psy, 2000, 48-50.
• 19. Touraine J.L., Gay-Ferret G., Sanadji K., Othmane O., Fournie G.J.: lsoprinosine: synergistic effects with NPT 15392 in vitro and activity on suppressor T-lymphocytes in autoimmune mice in vivo. In: Serrou B. et al. (Eds). Current Concept in Human Immunology and Cancer Immunomodulation. Elsevier Biomedical Press B.V., pp. 491-499, 1982.
• 20. Tsang K.Y. Fudenberg H.H., Galbraith G.M., Donnelly R.P., Bishop L.R. 1985. Partial restoration of impaired interleukin-2 production and Tac antigen (putative interleukin-2 receptor) expression in patients with acquired immune deficiency syndrome by isoprinosine treatment in vitro. J. Clin. Invest., 1985, 75, 1538-1544.
• 21. Van Kampen K.R.: Immunotherapy and cytokines. Semin. Vet. Med. Surg. (Small Animals), 1997, 12, 186-192.
• 22. Wagner U., Burkhardt E., Failing K.: Evaluation of canine lymphocyte proliferation: comparison of three different colorimetric methods with the Hthymidine incorporation assay. Vet. Immunol. and Immunopathol., 1999, 70, 151-159.
• 23. Wybran J., Appelboom T.: Isoprinosine (inosiplex): immunological and clinical effect. In: Fudenberg H.H. and Whitten H.D. (eds). Immunomodulation: New Frontiers and Advances. Plenum Press, New York, pp. 363-374, 1984