Optimization and purification of alkaline proteases produced by marine Bacillus sp. MIG newly isolated from Eastern Harbour of Alexandria

• Autorzy: Gouda M K
• Języki publikacji: EN

Abstrakty

EN

A marine Bacillus strain was isolated from the eastern harbour of Alexandria and identified as Bacillus sp. MIG. Maximum activity of studied proteases was obtained when the bacterium was grown in medium with 1 % wheat bran and 0.5% yeast extract in addition to the mineral salts and incubated for 48 h at 30°C and 120 rpm. Two alkaline proteases (Pro 1 and Pro 2) were purified to homogeneity using cation exchange chromatography on CM-Sepharose CL-6B followed by Sephadex G-75 superfine. The optimum activities were at pH 11 or 12, and temperatures of 50 and 55°C for Pro 1 and Pro 2 respectively. These two enzymes were relatively stable over pH range from 7.0-11. Pro 2 was found to be more stable at 50°C in absence of Ca²⁺ and retained about 47% of its activity after 3 h at this temperature, while Pro 1 lost its activity completely at the same conditions. The two enzymes were active against haemoglobin and casein; in addition, Pro 2 exhibited moderate activity against keratin. Both enzymes were partially inhibited by Ag⁺ and Hg²⁺. PMSF completely inhibited the enzymes, while dithiothreitol and 2-mercaptoethanol stimulated their activities, suggesting to be thiol-dependent serine proteases. The enzymes were stable in the presence of the surfactants and bleaching agent (H₂O₂) and relatively stable in presence of some commercial detergents.

Słowa kluczowe

EN

marine strain; production; optimization; alkaline protease; alkaline serine protease; Alexandria; thiol-dependent serine protease; purification; Bacillus; Egypt; Eastern Harbour

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 2
• Opis fizyczny: p.119-126,fig.,ref.

Twórcy

autor

Gouda M K

• Alexandria University, Alexandria, Egypt

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