Phenol degradation by bacteria. Part one: Isolation and characterization of bacterial strain
An effective microorganism for phenol degradation process was selected and isolated from the natural environment using basic screening. The tested strain Alcaligenes xylosoxidans ssp. denitrificans was compared in vital activity experiments to the CCM strain Pseudomonas putida 3423 in order to obtain optimal biochemical parameters and to find out the right way of cultivation for the preparation of an inoculum. The efficiency of phenol degradation at higher concentrations is strongly influenced by microorganism age and, of course, by the initial substrate concentration. The time of biodegradation with the increase of initial phenol concentration was increased, but to the critical concentration was independent of it. The method of preparation of an inoculum with highly induced oxygenase system was found. A suitable composition of the broth medium for precultivation and cultivation of tested microorganisms has resulted from this work.
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