Detection of Clostridium difficile and its toxin A [TcdA] in stool specimens from hospitalised patients

• Autorzy: Wroblewska M M , Swoboda-Kopec E. , Rokosz A. , Nurzynska G. , Bednarska A. , Luczak M.
• Języki publikacji: EN

Abstrakty

EN

The study has been carried out to determine the frequency of C. difficile recovery in stool cultures and the rate of C. difficile toxin A detection in faecal specimens of patients with nosocomial diarrhoea. Clinical specimens comprised 4414 stool samples collected from 1998 to 2002 from adult patients hospitalised in different wards of a university-affiliated hospital (1200 beds) and suspected of C. difficile-associated disease (CDAD). There have been 1308 (29.6%) specimens positive for C. difficile culture (15.1% in 1998, 29.5% in 1999, 33.8% in 2000, 31.2% in 2001 and 32.0% in 2002). The highest number of C. difficile strains was cultured from stool samples of patients hospitalised in the haematology/oncology ward (51.1% of all isolates), neurology (8.3%), nephrology (8.0%), gastrointestinal surgery (7.0%) and neurosurgery (6.2%) wards. The testing for C. difficile toxin A yielded 847 (19.2%) positive samples and 3567 (80.8%) toxin A-negative results. The percentage of C. difficile toxin A-positive samples was 29.4% in 1998, 17.5% in 1999, 23.2% in 2000, 17.1% in 2001 and 15.0% in 2002. In the analysed period we observed an increase in the number of stool specimens tested for C. difficile and an increase in the number of C. difficile culture-positive samples. A decrease in the number of C. difficile toxin A-positive samples was noted in the last 2 years of the study. This phenomenon may be due to an improved antibiotic policy of the hospital.

Słowa kluczowe

EN

medical microbiology; patient; stool specimen; toxin A; Clostridium difficile; detection

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 2
• Opis fizyczny: p.111-115,ref.

Twórcy

autor

Wroblewska M M

• Medical University of Warsaw, 5 Chalubinskiego Street, 02-004 Warsaw, Poland

autor

Swoboda-Kopec E.

autor

Rokosz A.

autor

Nurzynska G.

autor

Bednarska A.

autor

Luczak M.

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