Detection and sequencing of new cyclic peptides from linseed by electrospray ionization mass spectrometry

• Autorzy: Stefanowicz P.
• Języki publikacji: EN

Abstrakty

EN

Extract of Linum usitatissimum seeds was analyzed by ESI-MS and ESI-MS/MS. The analysis confirms the presence of previously reported cyclolinopeptides: CLA (c(Pro- Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val) and CLB (c(Pro-Pro-Phe-Phe-Val-Ile-Met-Ile-Leu)). Cyclolinopeptides CLD and CLE, which contain methionine oxide, were detected in small quantities only. These peptides likely result from the oxidation of their precur­sors, not reported previously: CLD' (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Leu)) and CLE'(c(Pro-Leu-Phe-Ile-Met-Leu-Val-Phe)), present at higher concentrations in unoxi- dized extract. Two new cyclic octapeptides: CLF (c(Pro-Phe-Phe-Trp-Val-Met-Leu- Met)) and CLG (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Met)) were detected and their se­quences were proposed on the basis of CID experiments and similarity with those of CLD.

Słowa kluczowe

EN

biological activity; cyclolinopeptide; peptide; mass spectrometry; Linum usitatissimum; detection

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 4
• Opis fizyczny: p.1125-1129,fig.

Twórcy

autor

Stefanowicz P.

• University of Wroclaw, F.Joliot-Curie 14, 50-383 Wroclaw, Poland

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