Microstructure and chemical composition of leaf cuticular waxes in two Salix species and their hybrid

• Autorzy: Szafranek B , Tomaszewski D. , Pokrzywinska K. , Golebiowski M.
• Języki publikacji: EN

Abstrakty

EN

Leaf epicuticular wax morphology and chemical composition of total cuticular waxes were studied in two Salix species (Salix alba and S.fragilis) and their hybrid (S. x rubens). A smooth wax layer with small, scattered wax structures covered the adaxial leaf surface in all three taxa, and a crustlike wax layer composed of terminally fused wax filaments was present on the abaxial surface. The leaf cuticular waxes, both epicuticular and intracuticular, were obtained by hot extraction in chloroform and then analyzed by gas chromatography and mass spectrometry. The principal components of the waxes were primary alcohols, fatty acids, aldehydes, n-alkanes and wax esters. The qualitative composition of the waxes was quite similar but there were quantitative differences between the taxa. The epicuticular crystalline waxes are composed of very-long-chain aldehyde polymers.

Słowa kluczowe

PL

willow; Salix; plant species; Salix alba; Salix fragilis; chemical analysis; wax extraction; microstructure; chemical composition; leaf; cuticular wax; hybrid; Salix x rubens; morphology; plant morphology

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2008
• Tom: 50
• Numer: 2
• Opis fizyczny: p.49-54,fig.,ref.

Twórcy

autor

Szafranek B

• Faculty of Chemistry, University of Gdansk, Sobieskiego 18/19, 80-952 Gdansk, Poland

autor

Tomaszewski D.

autor

Pokrzywinska K.

autor

Golebiowski M.

Bibliografia

• Baker EA. 1982. Chemistry and morphology of plant epicuticular waxes. In: Cutler DF, Alvin KL, and Price CE [eds.], The plant cuticle, 139-165. Academic Press, London.
• Barthlott W, Neinhuis C, Cutler D, Ditsch F, Meusel I, Theisen I, and Wilhelmi H. 1998. Botanical Journal of Linnean Society 126: 237-260.
• Bianchi G. 1995. Plant waxes. In: Hamilton RJ [ed.], Waxes: Chemistry, molecular biology and functions, 175-222. The Oily Press, Dundee, Scotland.
• Cameron KD, Teece MA, Bevilacqua E, and Smart LB. 2002. Diversity of cuticular wax among Salix species and Populus species hybrids. Phytochemistry 60: 715-725.
• Christiansen K, Mahadevan V, Viswanathan CV, and Holman RT. 1968. Mass spectrometry of long-chain aliphatic aldehydes, dimethyl acetals and alk-1-enyl ethers. Lipids 4: 421-427.
• Christie WW. 1994. Gas chromatography and lipids. The Oily Press, Ayr, Scotland.
• Ensikat HJ, Neinhuis C, and Barthlott W. 2000. Direct access to plant epicuticular wax crystals by a new mechanical isolation method. International Journal of Plant Sciences 161: 143-148.
• Evershed RP. 1992a. Gas chromatography of lipids. In: Hamilton RJ, and Hamilton S [eds.], Lipid analysis. A practical approach, 113-151. Oxford University Press, Oxford.
• Evershed RP. 1992b. Mass spectrometry of lipids. In: Hamilton RJ, and Hamilton S [eds.], Lipid analysis. A practical approach, 263-308. Oxford University Press, Oxford.
• Haas K, Brune T, and Rücker E. 2001. Epicuticular wax crystalloids in rice and sugar cane leaves are reinforced by polymeric aldehydes. Journal of Applied Botany 75: 178-187.
• Hietala T, Laakso S, and Rosenqvist H. 1995. Epicuticular waxes of Salix species in relation to their overwintering survival and biomass productivity. Phytochemistry 40: 23-27.
• Hietala T, Mozes N, Genet MJ, Rosenqvist H, and Laakso S. 1997. Surface lipids and their distribution on willow (Salix) leaves: a combined chemical, morphological and physicochemical study. Colloids and Surfaces B: Biointerfaces 8: 205-215.
• Jetter R, Schäffer S, and Riederer M. 2000. Leaf cuticular waxes are arranged in chemically and mechanically distinct layers: evidence from Prunus laurocerasus L. Plant, Cell and Environment 23: 619-628.
• Kitson FG, Larsen BS, and McEwen CN. 1996. Gas chromatography and mass spectrometry, 162-163. Academic Press, London.
• Little JL. 1999. Artifacts in trimethylsilyl derivatisation reactions and ways to avoid them. Journal of Chromatography A 844: 1-22.
• MSDC 1991. Eight peak index of mass spectra. The Royal Society of Chemistry, Cambridge.
• Nass R, Markstädter C, Hauke V, and Riederer M. 1998. Quantitative gas chromatographic analysis of plant cuticular waxes containing long-chain aldehydes. Phyto-chemical Analysis 9: 112-118.
• Riedel M, Eichner A, and Jetter R. 2003. Slippery surfaces of carnivorous plants: composition of epicuticular wax crystals in Nepenthes alata Blanco pitchers. Planta 218: 87-97.
• Riedel M, Eichner A, Meimberg H, and Jetter R. 2007. Chemical composition of epicuticular wax crystals on the slippery zone in pitchers of five Nepenthes species and hybrids. Planta 225: 1517-1534.
• Szafranek B, and Synak E. 2006. Cuticular waxes from potato (Solanum tuberosum) leaves. Phytochemistry 67: 80-90.
• Tomaszewski D. 2004. The wax layer and its morphological variability in four European Salix species. Flora 199: 320-326.
• Wettstein-Knowles P. 1995. Biosynthesis and genetics of waxes. In: Hamilton RJ [ed.], Waxes: Chemistry, molecular biology and functions, 91-130. The Oily Press, Dundee, Scotland.