The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo

• Autorzy: Kozak D

Warianty tytułu

PL

Wplyw retardantow wzrostu zastosowanych in vitro na aklimatyzacje i wzrost Tibouchina urvilleana Cogn. w warunkach in vivo

• Języki publikacji: EN

Abstrakty

EN

Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg·dm⁻³, flurprimidol – 0.1, 1.0, 5.0 mg·dm⁻³, chlorocholine chloride (CCC) – 5.0, 50.0, 250.0 mg·dm⁻³. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat : 1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg·dm⁻³. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg·dm⁻³ inhibited very strong growth of plants after 10 weeks of growth ex vitro.

PL

Wierzchołki pędów Tibouchina urvilleana Cogn. prowadzono 4 tygodnie in vitro na zmodyfikowanej pożywce Murashige i Skooga (MS) [1962] z dodatkiem retardantów wzrostu: paklobutrazol – 0,1; 0,5; 1,0; 5,0 mg·dm⁻³, fluropirimidol – 0,1; 1,0; 5,0 mg·dm⁻³, chlorek chlorocholiny (CCC) – 5,0; 50,0; 250,0 mg·dm⁻³. Kontrolę stanowiła pożywka nie zawierająca retardantów wzrostu. Ukorzenione pędy przenoszono do szklarni i sadzono do mieszaniny torfu i perlitu (1:1), gdzie rosły przez 5 tygodni. Następnie rośliny przesadzono do substratu torfowego i uprawiano przez kolejne 5 tygodni. Rośliny przyjęły się w 92,5–100,0%. Najniższy procent przyjęć wykazywały rośliny pochodzące z pożywki zawierającej 5,0 mg·dm⁻³ fluropirimidolu. Wzrost in vitro pędów Tibouchina urvilleana na pożywce zawierającej retardanty wzrostu miał istotny wpływ na dalszy wzrost roślin w podłożu. Paklobutrazol i fluropirimidol w stężeniu 5,0 mg·dm⁻³ bardzo silnie hamowały wzrost roślin ex vitro po 10 tygodniach.

Słowa kluczowe

EN

in vitro; acclimatization; growth retardant; plant cultivation; growth; in vivo; Tibouchina urvilleana

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2006
• Tom: 05
• Numer: 1
• Opis fizyczny: p.65-70,ref.

Twórcy

autor

Kozak D

• Agricultural University of Lublin, Leszczynskiego 58, 20-068 Lublin, Poland

Bibliografia

• Eliasson M. K., Beyl C. A., Barker P. A., 1994. In vitro responses and acclimatization of Prunus serotina with paclobutrazol. J. Plant Growth Regulation 13, 137–142.
• Gribaudo I., Novello V., Restagno M., 2001. Improved control of water loss from micropropagated grapevines (Vitis vinifera cv. Nebbiolo). Vitis 40, 3, 137–140.
• Henderson K. P., Taji A. M., Williams R. R., 1994. Effect of reduced humidity and paclobutrazol on acclimatization of tissue-culture plants. Inter. Plant Propag. Soc. Comb. Proc. 43, 97–102.
• Kozak D., Grodek J., 2005. The consequent effect of growth retardants on the growth and development of Tibouchina urvilleana Cogn. shoots in vitro. Acta Sci. Pol. Hortorum Cultus 4 (2), 123–128.
• Larsen R. U., Lieth J. H., 1993. Shoot elongation retardation owing to daminozide in chrysanthemum: I. Modeling single applications. Scientia Hort. 1–2, 109–125.
• Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
• Novello V., Roberts A. V., 1992. Effects of paclobutrazol and reduced humidity on stomatal conductance of micropropagated grapevines. Acta Hort. 319, 65–70.
• Oliphant J., 1990. The use of paclobutrazol in the rooting medium of micropropagated plants. Comb. Proc. Int. Plant Prop. Soc. 40, 358–360.
• Roberts A.V., Matthews D., 1995. The preparation in vitro of chrysanthemum for transplantation to soil. 5. The 2S, 3S enantiomer of paclobutrazol improves resistance to desiccation. Plant Cell Tissue and Organ Culture 40, 2, 191–193
• Roberts A. V., Walker S., Horan I., Smith E. F., Mottley J., 1992. The effect of growth retardants, humidity and lighting at stage III on stage IV of micropropagation in chrysanthemum and rose. Acta Hort. 319, 153–158.
• Ruter J. M., 1994. Growth and landscape establishment of Pyracantha and Juniperus after application of paclobutrazol. HortScience 29, 11, 1318–1320.
• Smith E. F., Gribaudo I., Roberts A. V., Mottley J., 1992. Paclobutrazol and reduced humidity improve resistance of micropropagated grapevine to wilting. HortScience 27, 111–113.
• Ziv M., 1992. The use of growth retardants for the regulation and acclimatization of in vitro plants. Proceed. of the 14 Intern. Conf. on Plant Growth Subst., Amsterdam, 21–26 July, 199, 809–817.
• Ziv M., Ariel T., 1991. Bud proliferation and plant regeneration in liquid-cultured philodendron treated with ancymidol and paclobutrazol. J. Plant Growth Regulation 10, 53–57.
• Ziv M., Kahany S., Lilien-Kipnis H., 1994. Scaled-up proliferation and regeneration of Nerine in liquid cultures. Part I. The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors. Plant Cell Tissue and Organ Culture 39, 109–115.