Wplyw oksytetracykliny na aktywnosc metaboliczna i fagocytarna makrofagow oraz odpowiedz proliferacyjna limfocytow karpia i suma europejskiego

• Autorzy: Terech-Majewska E , Siwicki A.K.

Warianty tytułu

EN

Influence of oxytetracycline on the metabolic and phagocyte activity of macrophages and the proliferative response of lymphocytes in carp and European catfish

• Języki publikacji: PL

Abstrakty

EN

Oxytetracycline (OTC) is an antibiotic used in fish farming in many countries. It is an effective drug against many bacterial diseases occurring in breeding conditions. It is believed that OTC induces immunosuppression in humans and many animal species. The aim of the study was to examine the influence of OTC on the basic functions of Carp (Cyprinus carpio) and European Catfish (Silurus glanis) immune systems. The study used 200 Carp fry (50-100 g body weight) and 200 Catfish fry (80-100 g body weight). The fish were kept in plastic tanks (500-1000 litre capacity) in a water temperature of 20-22°C. OTC was applied by intraperitoneal injection at a dose of 10 mg/kg body weight. The results of the study indicate that OTC induces the suppression of metabolic (Respiratory Burst Activity) and phagocyte function (Phagocyte Killing Activity) in Carp and European Catfish macrophages, as well as increasing the proliferative response of lymphocytes. The alteration dynamics clearly indicate that suppression is temporary. In practice OTC is used in 75-100 mg/kg body weight doses over a couple of days (Carp, Catfish, and Trout). Longer suppression may make the fish susceptible to secondary infections.

Słowa kluczowe

PL

leukocyty; limfocyty; oksytetracyklina; karpie; odpowiedz proliferacyjna; aktywnosc fagocytarna; sum europejski; immunologia; aktywnosc metaboliczna; ryby; immunosupresja

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 12
• Opis fizyczny: s.1431-1434,tab.,bibliogr.

Twórcy

autor

Terech-Majewska E

• Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 13, 10-718 Olsztyn

autor

Siwicki A.K.

Bibliografia

• 1.Austin B.: Antibiotic pollution from fish farms: effect on aquatic microflora. Microbiol. Sci. 1985, 2, 113-117.
• 2.Banck G., Forsgren A.: Antibiotics and suppression of lymphocyte function in vitro. Antimicrob. Agent Chemother. 1979, 11, 544-560.
• 3.Dunier M., Siwicki A. K.: Effects of environmental contaminants and chemotherapeutics on fish defense mechanisms. Arch. Ryb. Pol. 1994, l. 2, 21-54.
• 4.Ervik A., Thorsen B., Eriksen V., Lunestad B. T., Samuelsen O. B.: Impact of administering antibacterial agents on wild fish and blue mussels Mytilus edulis in the vicinity of fish farms. Dis. Aquatic Organ. 1994, 18, 45-51.
• 5.Forsgren A., Gnarpe H.: The effect of antibacterial agents on the association between bacteria and leukocytes. Scand. J. Infect. Dis. 1982, 33 (suppl.), 115- -120.
• 6.Forsgren A., Schmelling D., Quie P. G.: Effect of tetracycline on the phagocytic function of human leukocytes. J. Infect. Dis. 1974, 130, 415-417.
• 7.Gnarpe H., Belsheim J.: Direct and indirect effects of antibiotics on granulocyte activity. J. Antimicrob. Chemother. 1981, 8 (Suppl. C), 71-78.
• 8.Gleichmann E., Kimber I., Purchase I. F.: Immunotoxicology: supressive and stimulatory effects of drugs and environmental chemicals on the immune system. Arch. Toxic. 1989, 63, 257-273.
• 9.Grondel J. L., Gloudemans A. G. M., van Muiswinkel W. B.: The influence of antibiotics on the immune system. II. Modulation of fish leukocyte response in culture. Vet. Immunol. Immunopathol. 1985, 9, 251-260.
• 10.Grondel J. L., Nouws J. F. M., Jong de Schutte A. R., Driessens F.: Pharmacokinetics and tissue distribution of oxytetracycline in carp, Cyprinus carpio L., following different routes of administration. J. Fish Dis. 1987, 10, 153-163.
• 11.Grondel J. L., Nouws J. F. M., Van Muiswinkel W. B.: The influence of antibiotics on the immune system. Immunopharmacokinetic investigations on the primary anty-SRBC response in carp Cyprinus carpio L. after oxytetracycline injection. J. Fish Dis. 1987, 10, 35-44.
• 12.Hektoen H., Berge J. A., Nygaard K., Schanning M., Hormazabal V., Yndestad M.: The environmental effects of antibacterial agents used in fish farming. Norsk-Veterinaertidsskrift 1993, 105, 27-32.
• 13.Hektoen H., Berge I. A., Hormazabal V., Yndestad M.: Persistence of antibacterial agents in marine sediments. Aguaculture 1995, 133, 175-184.
• 14.Ingram G. A.: Substances involued in the natural resistance of fish to infection. J. Fish Biol. 1980, 16, 23-60.
• 15.Jacobsen H., Berlind L.: Persistence of oxytetracycline in sediments from fish farms. Aquaculture 1988, 70, 365-370.
• 16.Kerry J., Hiney M., Coyne R., Nic Gabhain S., Gilroy D., Cazabon, Smith P.: Fish feed as sourse of oxytetracycline resistant bacteria in the sediments under fish farms. Aquaculture 1995, 131, 101-113.
• 17.Klaver Al., Matthews R. A.: Effects of oxytetracycline on nitrification in a model aquatic system. Aquaculture 1994, 123, 237-247.
• 18.Kwiatkowska K., Sobota A.: Przekazywanie sygnału fagocytarnego: od agregacji receptorów do przebudowy cytoszkieletu. Post. Biol. Kom. 1999, 26, 59-81.
• 19.Lunden T., Bylund G.: The influence of in vitro an in vivo exposure to antibiotics on mitogen induced proliferation of lymphoid cells in rainbow trout (Oncorhynchys mykiss). Fish Shellfish Immunol. 2000, 10, 395-404.
• 20.Lunden T., Lilius E. M., Bylund G.: Respiratory burst activity of rainbow trout (Oncorhynchys mykiss) phagocytes is modulated by antimicrobial drugs. Aquaculture 2002, 207, 203-212. 21.£apiñska K., Dąbrowski W., Daczkowska-Kazon E., Sawicki W.: Przyswajalność i okres karencji oksytetracykliny u pstrąga tęczowego. Medycyna Wet. 2005, 61, 466-470.
• 22.Myers M. J., Farrell D. E., Henderson M.: Oxytetracycline-mediated alteration of murine immunocompetence. Pathobiol. 1995, 65, 270-271.
• 23.Mosmann T.: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
• 24.Noga E. J.: Fish Disease Diagnosis and Treatment. Mosby-Year Book Inc., USA 1996, 276-277.
• 25.Radomska M.: Antybiotyki a układ odpornościowy. Reumatologia 1994, 32, 63-69.
• 26.Rook G. A., Steele J., Umar S., Dockrell H. M.: A simple method for the solubilisation of reduced NBT and its use as a colorimetric assay for activation of human macrophages by g-IFN. J. Immunol. Methods 1985, 82, 161-167.
• 27.Samuelsen B. O., Torsvik V., Ervik A.: Long-range changes in oxytetracycline concetration and bacterial resistance towards oxytetracycline in a fish farm sediment after medication. Sci. Total Environ. 1992, 114, 25-36.
• 28.Sierosławska A., Terech-Majewska E., Siwicki A. K.: Wpływ chemioterapeutyków na układ odpornościowy ryb, [w:] Ochrona zdrowia ryb aktualne problemy. Wyd. IRŻ, Olsztyn 2004, 227-235.
• 29.Siwicki A. K., Anderson D. P.: Nonspecific defence mechanisms assay in fish. II. Potential killing activity of neutrophils and monocytes, lysozyme activity in serum and organs and total immunoglobulin (Ig) level in serum, [w:] Siwicki A. K., Anderson D. P., Waluga J. (red.): Fish Diseases Diagnosis and Preventions Methods. Wyd. IRŻ, Olsztyn 1993, 105-112.
• 30.Siwicki A. K., Anderson D. P., Dunier M.: In vitro immunotoxicology alternative models for environmental toxicology. Biologiczne Monitorowanie Skażenia środowiska. Wyd. IRŒ Olsztyn, 1996, 159-170.
• 31.Siwicki A. K, Rymuszka A., Głąbski E., Terech-Majewska E.: Białka ostrej fazy u ryb możliwości zastosowania w monitorowaniu skażenia środowiska, [w:] Biologiczne Monitorowanie Skażenia środowiska. Wyd. IRŻ Olsztyn 1996, s. 173-177.
• 32.Studnicka M., Siwicki A. K., Morand M., Rymuszka A., Bownik A., Terech- -Majewska E.: Modulation of nonspecific defence mechanisms and specific immune responses after suppression induced by xenobiotics. J. Appl. Ichthyol. 2000, 16, 1-7.
• 33.Bogert C. Van den, Kroon A. M.: Effects of oxytetracycline on in vivo proliferation and differentiation of erythroid and lymphoid cells in the rat. Clin. Exp. Immunol. 1982, 50, 327-335.