EN
The objective of the study was to examine the potential of Yarrowia lipolytica A-101-1.31 growth control via the concentration of the nitrogen, phosphorus or sulphur source, as well as to assess the efficiency of each source separately and under simultaneous deficiency of nitrogen and phosphorus. The experimental study has produced the following findings: nitrogen, phosphorus and sulphur enabled the control of Y.lipolytica A-101-1.31 growth. Citric acid biosynthesis occurred irrespective of the growth-limiting factor used. The yeast cells produced citric acid with the desired efficiency only under conditions of nitrogen (qCAg=0.071 gg-1h-1) or sulphur (YCAP=0.74 gg-1) deficiency. The smallest changes in the elemental composition were observed when the yeast grew under nitrogen limitation. Elemental composition variations were the most distinct when the yeast biomass was cultivated under phosphorus deficiency. A low deficiency of phosphorus in the nitrogen deficient had a stimulating effect on the acid-producing of the growing cells (qCAg=0.089 gg-1h-1).