An improvement of cucumber cotyledon greening bioassay for cytokinins

• Autorzy: Yu X , Li G. , Xu D. , Dong X. , Qi X. , Deng Y.
• Języki publikacji: EN

Abstrakty

EN

The cucumber cotyledon greening bioassay for cytokinins was modified by using 95 % acetone -ethanol in stead of 80 % acetone as ex traction solvent. The cotyledons were ex tracted directly with a 2:1 (v/v) ac e tone-ethanol solution in dark for 24 hours, omitting the homogenization and centrifugation operations of the previous bioassay. The modified bioassay is more convenient and especially use ful in screening cytokinin-active substance from a large number of samples.

Słowa kluczowe

EN

chlorophyll extraction; cotyledon greening bioassay; N,N'-diphenylurea; cytokinin; cucumber; 6-benzyladenine

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2006
• Tom: 28
• Numer: 1
• Opis fizyczny: p.9-11,fig.,ref.

Twórcy

autor

Yu X

• Chinese Academy of Sciences, Lanzhou 730000, P.R.China

autor

Li G.

autor

Xu D.

autor

Dong X.

autor

Qi X.

autor

Deng Y.

Bibliografia

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• Fletcher R.A., Kalldumbil V., Steele P. 1980. An improved bioassay for cytokinins using cucumber cotyledons. Plant Physiol., 69: 675-677.
• Klepper R.R., Mcquirk P.A.1995. Investigation of cytokinin activity in a commercial plant growth regulator by means of a cucumber cotyledon chlorophyll production bioassay. Proc.Plant Growth Regul. Soc. Am., 22: 264-267.
• Min-wen Yang 2002. Study on rapid determination of chlorophyll content of leaves. Chinese Journal of Spectroscopy Laboratory 19: 478-481.
• Ricci A., Carra A., Torelli A., Maggiali C.A., Vicini P, Zani F., Branca C. 2001. Cytokinin-like activity of N-substituted N-phenylureas. Plant Growth Regulation, 34: 167-172.
• Wei-lun Chen, Yan-ru Song, Cheng Tsui 1979. Comparative study on the bioassay between N6, O2’’-dibutyryl cyclic adenosine 3,5-monophosphate and cytokinin. Acta Botanica Sinic, 21: 263-268.