Effect of protoplast source and media on growth and regenerability of protoplast-derived calluses of Solanum tuberosum L.

• Autorzy: Anjum M A
• Języki publikacji: EN

Abstrakty

EN

Freshly isolated mesophyll and suspensions-cell protoplasts of S. tuberosum cvs. Desiree and Maris Piper were cultured in different media i.e. modified MS, V-KM and MS-KM. Protoplast plating efficiencies were higher in MS-KM medium. Resulting protoplast-derived calluses were transferred either onto the medium of Bokelmann and Roest (1983) or that of Lam (1977) for shoot regeneration. Calluses derived from mesophyll cell protoplasts differentiated about 2 weeks earlier than calluses derived from suspension-cell protoplasts. Shoot initiation was also about 2 weeks earlier from calluses subcultured onto the former medium as compared to the latter.

Słowa kluczowe

EN

plant regeneration; callus; Solanum tuberosum; protoplast; potato; growth

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1998
• Tom: 20
• Numer: 2
• Opis fizyczny: p.129-133

Twórcy

autor

Anjum M A

• University College of Agriculture, Bahauddin Zakariya University, Multan-60800, Pakistan

Bibliografia

• Anjum, M.A. 1994. Tissue culture and frost tolerance studies in Solanum. Ph.D. Thesis, University of Salford.
• Avetisov, V.A., Krivoshchapova, G.I., Melik-Sarkisov, O.S. 1987. Some characteristics of potato callus protoplast culture. Soviet Plant Physiol., 34: 484–490.
• Binding, H. 1974. Regeneration of haploid and diploid plants from protoplasts of Petunia hybrida L. Z. Pflanzenphysiol., 74:327–356.
• Bokelmann, G.S., Roest, S. 1983. Plant regeneration from protoplasts of potato (Solanum tuberosum cv. Bintje). Z. Pflanzenphysiol., 109: 259–265.
• Briza, J., Machova, I. 1991. Regeneration of plants from leaf mesophyll protoplasts of tetraploid potato cultivars, Xenia and Bintje. Biol. Plant., 33: 225–233.
• Cardi, T., Puite, K.J., Sree Ramulu, K. 1990. Plant regeneration from mesophyll protoplasts of Solanum commersonii Dun. Plant Sci., 70:215–221.
• De Vries, S.E., Bokelmann, G.S. 1986. Regeneration of callus and plants from cell suspension protoplasts of dihaploid potato (Solanum tuberosum L.). J. Plant Physiol., 122: 199–203.
• Espinoza, N., Estrada, R., Tovar, P., Bryan, J. and Dodds, J.H. 1986. Tissue culture micropropagation, conservation and export of potato germplasm. Specialized Technology Document 1. International Potato Centre, Lima, Peru: 1–20.
• Feher, A., Preiszner, J., Dudits, D. 1989. Differentiation of potato (Solanum tuberosum L.) plants from cultured leaf protoplasts. Acta Biol. Hung. 40:369–380.
• Jones, H., Karp, A., Jones, M.G.K. 1989. Isolation, culture and regeneration of plants from potato protoplasts. Plant Cell Rep., 8: 307–311.
• Kikuta, Y., Fujino, K., Saito, W. 1986. Protoplast culture of potato: an improved procedure for isolating viable protoplasts. J. Fac. Agric., Hokkaido Univ., 62:429–439.
• Lam, S.L. 1977. Regeneration of plantlets from single cells in potatoes. Amer. Potato J., 54:575–580.
• Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473–479.
• Shepard, J.F., Totten, R.E. 1977. Mesophyll cell protoplasts of potato. Plant Physiol., 60: 313–316.
• Tan, M-L.M.C., Boerrigter, H.S., Kool, Ad.J. 1987. A rapid procedure for plant regeneration from protoplasts isolated from suspension cultures and leaf mesophyll cells of wild Solanum species and Lycopersicon pennellii. Plant Sci., 49: 63–72.
• Tavazza, R., Ancora, G. 1986. Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desiree). Plant Cell Rep., 5: 243–246.
• Tavazza, R., Ordas, R.J., Ancora, G. 1988. Procedure for the regeneration of plants from cell suspension protoplasts of tetraploid potato (Solanum tuberosum L.) cv. Desiree. Plant Sci., 58: 223–230.