Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria

• Autorzy: Adiguzel A , Ozer H. , Sokmen M. , Gulluce M. , Sokmen A. , Kilic H. , Sahin F. , Baris O.
• Języki publikacji: EN

Abstrakty

EN

Catnip (Nepeta cataria) is an important medicinal herb belonging to the mint family, Lamiaceae. In this study, the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Nepeta cataria, and its essential oil composition were investigated. The essential oil, which has 4aα,7α,7aβ-nepetalactone (70.4%), 4aα,7α,7aβ-nepetalactone (6.0%), thymol (2.3%), and 4aα,7α, 7aβ-nepetalactone (2.5%), as main components, exhibited activity against eleven bacteria, and twelve fungi and a yeast, C. albicans; with Minimum Inhibitory Concentrations (MIC) values ranging from 12.50 to 250 μl/ml; the methanol extract showed weaker activity. The samples were also subjected to a screening for their possible antioxidant activities by using 2.2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid assays. In DPPH assay, the extract showed slight antioxidant activity whereas the essential oil remained inactive. In the latter case, both the extract and the essential oil exerted weak activity having inhibiton ratios of linoleic acid oxidation at 16.4% and 27.0%, respectively. The weak antioxidative nature of the extract could be attributed to the low phenolic content, estimated as gallic acid equivalent at 22.6 ± 2.07 μg/ml or 2.26%. In both systems, antioxidant capacity of BHT was determined in parallel experiments.

Słowa kluczowe

EN

mass spectrometry; essential oil; catnip; antioxidant activity; antimicrobial activity; gas chromatography; Nepeta cataria; methanol extract

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 1
• Opis fizyczny: p.69-76,ref.

Twórcy

autor

Adiguzel A

• Health Services Vocational Training School, Ataturk University, Yenisehir-Erzurum, 25070, Turkey

autor

Ozer H.

autor

Sokmen M.

autor

Gulluce M.

autor

Sokmen A.

autor

Kilic H.

autor

Sahin F.

autor

Baris O.

Bibliografia

• Adams R.P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th Edition. Allured Publishing Corp., Carol Stream, IL, USA.
• Angelini P., R. Pagiotti, A. Menghini and B. Vianello. 2006. Antimicrobial activities of various essential oils against foodborne pathogenic or spoilage moulds. Ann. Microbiol. 56: 65-69.
• Baser K.H.C., N. Kirimer, M. Kurkcuoglu and B. Demirci. 2000. Essential oils of Nepeta species growing in Turkey. Chem. Nat. Compd. 36: 356-359.
• Bourrel C, F. Perineau, G. Michel and J.M. Bessiere. 1993. Catnip (Nepeta cataria L.) essential oil: analysis of chemical constituents, bacteriostatic and fungistatic properties. J. Essent. Oil Res. 5: 159-167.
• Burits M. and F. Bucar. 2000. Antioxidant activity of Nigella sativa essential oil. Phytother. Res. 14: 323-328.
• Chandler S.F. and J.H. Dodds. 1983. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum lacinitum. Plant Cell Rep. 2: 105-108.
• Cozzi R., R. Ricordy, T. Aglitti, V. Gatta, P. Petricone and R. De Salvia. 1997. Ascorbic acid and beta-carotene as modulators of oxidative damage. Carcinogenesis 18: 223-228.
• Cuendet M., K. Hostettmann, O. Potterat and W. Dyatmyko. 1997. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv. Chim. Acta 80: 1144-1152.
• Dapkevicius A., R. Venskutonis, T.A. Van Beek and J.P.H. Linssen. 1998. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J. Sci. Food Agr. 77: 140-146.
• Davis P.H. 1982. Flora of Turkey and the East Aegean Islands, pp. 270-271, vol.7, Edinburg University Press, Edinburg.
• Duke J.A. 1987. CRC Handbook of Medicinal Herbs, 5th ed„ CRC Press, Boca Raton, FL.
• Flamini G., P.L. Cioni, R. Puleio, I. Morelli and L. Panizzi. 1999. Antimicrobial activity of the essential oil of Calamintha nepeta and its constituent pulegone against bacteria and fungi. Phytother. Res. 13: 349-351.
• Galati E.M., N. Miceli, M. Galluzzo, M.F Taviano and O. Tzakou. 2004. Neuropharmacological effects of epinepetalactone from Nepeta sibthorpii behavioral and anticonvulsant activity. Pharm. Biology. 42: 391-395.
• Ganzera M., S. Crockett, M.R. Tellez and I.A. Khan. 2001. Determination of nepetalactone in Nepeta cataria by reversed phase high performance liquid chromatography. Pharmazie 56: 896-897.
• Gul H.I., T. Ojanen and O. Hanninen. 2002. Antifungal evaluation of bis Mannich bases derived from acetophenones and their corresponding piperidinols and stability studies. Biol. Pharm. Bull. 25: 1307-1310.
• Gulluce M., F. Sahin, M. Sokmen, H. Ozer, D. Daferera, A. Sokmen, M. Polissiou, A. Adiguzel and H. Ozkan. 2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp longifolia. Food Chem. 103:1449-1456
• Gulluce M., M. Sokmen, F. Sahin, A. Sokmen, A. Adiguzel and H. Ozer. 2004. Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L) Druce ssp serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. J. Sci. Food Agr. 84: 735-741.
• Handjieva N.V., S.S. Popov and L.N. Evstatieva. 1996. Constituents of essential oils from Nepeta cataria L., N. grandiflora M.B. and N. nuda L. J. Essent. Oil Res. 8: 639-643.
• Hethelyi E.B., L.G. Szabo and K. Korany. 2001. Volatile constituents of Nepeta cataria L., N. glechoma Benth. and N. parviflora M. Bieb. from Hungary. Int. J. Hortic. Sci. 7: 47-50.
• Jamzad Z., R.J. Grayer, G.C. Kite, M.S.J. Simmonds, M. Ingrouille and A. Jalili. 2003. Leaf surface flavonoids in Iranian species of Nepeta (Lamiaceae) and some related genera. Biochem. Syst. Ecol. 31: 587-600.
• Kokdil G., S.M. Yalcin and G. Topcu. 1999. Nepetalactones and other constituents of Nepeta nuda ssp. albiflora. Turkish J. of Chem. 23: 99-104.
• Kordali S., R. Kotan, A. Mavi, A. Cakir, A. Ala and A. Yildirim. 2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. J. Agric. Food Chem. 53: 9452-9458.
• Lee K.G. and T. Shibamoto. 2001. Antioxidant activities of volatile components isolated from Eucalyptus species. J. Sci. Food Agric. 81: 1573-1579.
• Malizia R.A., J.S. MolliJ, D.A. Cardell and J.A. Retamar. 1996. Volatile constituents of the essential oil of Nepeta cataria grown in Cordoba Province (Argentinia). J. Essent. Oil Res. 8: 565-567.
• Morteza-Semnani K. and M. Saeedi. 2004. Essential oils composition of Nepeta cataria L. and Nepeta crassifolia Boiss. and Buhse from Iran. J. Essent. Oil-Bear. Plants 7: 120-124.
• Murray P.R., E.J. Baron, M.A. Pfaller, F.C. Tenover and R.H. Yolke. 1995. Manual of Clinical Microbiology, Vol. 6. ASM, Washington, DC.
• Nostro A., M.P. Germano, V. D'angelo, A. Marino and M.A. C'annatelli. 2000. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol. 30: 379-384.
• Ozer H., M. Sokmen, M. Gulluce, A. Adiguzel, F. Sahin, A. Sokmen, H. Kilic and O. Boris. 2007. Chemical composition and antimicrobial and antioxidant activities of the essential oil and methanol extract of Hippomarathrum microcarpum (Bieb.) from Turkey. J. Agric. Food Chem. 55: 937-942.
• Peterson C.J. and J. Ems-Wilson. 2003. Catnip essential oil as a barrier to subterranean termites (Isoptera: Rhinotermitidae) in the laboratory. J. Econ. Entomol. 96: 1275-1282.
• Slinkard K. and V.L. Singleton. 1977. Total phenol analyses: automation and comparison with manual methods. Am. J. Enol. Vitic. 28: 49-55.
• Small E. 1997. Culinary Herbs, pp. 441-449. In: National Research Council Press (eds), Ottawa, Ontario, Canada.
• Sonboli A., P. Salehi and M. Yousefzadi. M. 2004. Antimicrobial activity and chemical composition of the essential oil of Nepeta crispa Willd. from Iran. Z. Naturforsch. C J. Biosci. 59: 653-656.
• Stojanovic G., N. Radulovic, J. Lazarevic, D. Miladinovic and D. Dokovic. 2005. Antimicrobial activity of Nepeta rtanjensis essential oil. J. Essent. Oil Res. 17: 587-589.
• Suschke U., H.K. Geiss and J. Reichling. 2006. Antibacterial activity of the essential oils of catnip (Nepeta cataria L.) and lemon balm (Melissa officinalis L.) against clinical isolates from the respiratory tract. Planta Med. 72: 992.
• Takeda Y., Y. Kiba, T. Masuda, H. Otsuka, G. Honda, M. Tagawa, E. Sezik and E. Yesilada. 1999. Nepetaracemosides A and B, iridoid glucosides from Nepeta racemosa. Chem. Pharm. Bull. 47: 1433-1435.
• Tepe B., M. Sokmen, A. Sokmen, D. Dafercra and M. Polissiou. 2005. Antimicrobial and antioxidative activity of the essential oil and various extracts of Cyclotrichium origanifolium (Labill.) Manden & Scheng. J. Food Eng. 69: 335-342.
• Tepe B., D. Daferera, A. Sihoglu-Tepe, M. Polissiou and A. Sokmen. 2007. Antioxidant activity of the essential oil and various extracts of Nepeta ?avida Hub.-Mor. from Turkey. Food Chem. 103: 1358-1364.
• Wang M.F., K.W. Cheng, Q.L. Wu and J.E. Simon. 2007. Quantification of nepetalactones in catnip (Nepeta cataria L.) by HPLC coupled with ultraviolet and mass spectrometry detection. Phytochem. Anal. 18: 157-160.