Spermiogenesis and sperm ultrastructure of the liver fluke Fasciola hepatica L., 1758 [Digenea, Fasciolidae]: transmission and scanning electron microscopy, and tubulin immunocytochemistry

• Autorzy: Ndiaye P I , Miquel J , Fons R , Marchand B
• Języki publikacji: EN

Abstrakty

EN

We describe here the ultrastructure of spermiogenesis and the mature spermatozoon of Fasciola hepatica (Trematoda, Digenet Fasciolidae) by means of scanning (SEM) and transmission electron microscopy (TEM). Spermiogenesis in F. hepatica follows the general pattern of digeneans. However, this is the first report of a flagellar rotation of about 120° in Digenea. The mature spermatozoon of F. hepatica is a filiform cell of about 275 µm in length. SEM and whole mount TEM revealed a helical pattern of axonemes around the sperm body. The spermatozoon presented the same ultrastructural organization as the congenent F. gigantica. The most interesting features of the male gamete of F. hepatica therefore are the dorsolateral cytoplasmic expansion, the external ornamentation of the cell membrane and the spine-like bodies. We also analysed the distribution pattern of tubulin in the microtubular cytoskeleton of F. hepatica by means of monoclonal anti-tubulins (anti-α-tubulin, anti-β-tubulin, anti-aα -acetylated tubulin and anti-α-tyrosinated tubulin). These anti-tubulins labelled axonemal and cortical microtubules but not the central core of the 9+‘1’ trepaxonematan axoneme.

Słowa kluczowe

EN

Fasciolidae; sperm ultrastructure; transmission electron microscopy; immunocytochemistry; spermatozoon; scanning electron microscopy; Fasciola hepatica; transmission; Digenea; liver fluke; spermiogenesis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2003
• Tom: 48
• Numer: 3
• Opis fizyczny: p.182-194,fig.

Twórcy

autor

Ndiaye P I

• Laboratori de Parasitologia, Department de Microbiologia i Parasitologia Sanitaries, Facultat de Farmacia, Universitat de Barcelona, Av.Joan XXIII, sn, E-08028 Barcelona, Spain

autor

Miquel J

• Laboratori de Parasitologia, Department de Microbiologia i Parasitologia Sanitaries, Facultat de Farmacia, Universitat de Barcelona, Av.Joan XXIII, sn, E-08028 Barcelona, Spain

autor

Fons R

• Department d'Ecologie Evolutive, Laboratoire Arago, CNRS UMR 7628, F-66650 Banyllus-sur-Mer, France

autor

Marchand B

• Laboratoire Parasites et Ecosytemes Mediterraneens, Faculte des Sciences et Techniques, Universite de Corse, F-20250 Corte, France

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