Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

• Autorzy: Kurnatowska A , Bialasiewicz D.
• Języki publikacji: EN

Abstrakty

EN

Hydrolase activity of (enzymograms, biotypes) in Geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. Axenic strains were differentiated with API 20C Aux and API ZYM tests. Then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. High variability of enzymograms (17 different types) was found. The highest activity was noted in case of: e2-alkaline phosphatase, e6-lecucine arylamidase, e11-acid phosphatase. E5-lipase. e7-valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase and e17-β-glucosidase were used for biotyping procedures. Our own system of biotyping of 81 strains of G. candidum was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4: 1) – all; ,,+”;one „-”, three „+”; two „-”,two „+”; three ,,-”,one ,,+”; all „-”. We have found: A (11.1 ± 3.5%), B1 (6.17 ± 2.67%). B2 (1.23 ± 1.22%), B4 (4.94 ± 2.41%), C1 (1.23 ±1.22%), C3 (63.0 ± 5.4%), D2 (9.88 ±3.31%), D3 (2.47 ± 1.72%). Among all strains from 8 various biotypes of G. candidum.

Słowa kluczowe

EN

hydrolase; biotyping; hydrolase activity; gastrointestinal tract; Geotrichum candidum

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 4
• Opis fizyczny: p.851-857,ref.

Twórcy

autor

Kurnatowska A

• Medical University of Lodz, Kosciuszki 85, 90-436 Lodz, Poland

autor

Bialasiewicz D.

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