The influence of mercury on computer analyzed sperm motility of common carp, Cyprinus carpio L., in vitro

• Autorzy: Chyb J , Sokolowska-Mikolajczyk M. , Kime D.E. , Socha M. , Epler P.
• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to evaluate the impact of different mercury concentrations (ranging from 0.05 to 1 ppm) on common carp sperm motility. Computer assisted sperm analysis (CASA) and microscopic observations of the time of sperm motility were applied in the experiment. According to analysis using the CASA method, it was found that all the tested concentrations of mercury decreased sperm motility, and that lethal effects were observed at concentrations of 0.5 and 1 ppm. On the other hand, microscopic observations showed that mercury decreased the time of sperm movement at doses as low as 0.2 ppm, and that lethal effects were evident at 1ppm. These results suggest the negative impact of mercury on sperm motility. The reasons for the differences in the results obtained using these two techniques are also discussed.

PL

Celem niniejszej pracy było zbadanie wpływu różnych stężeń rtęci (od 0,05 do 1 ppm) na ruchliwość plemników karpia. W badaniach wykorzystano zarówno komputerową analizę ruchliwości plemników (CASA), jak i subiektywne mikroskopowe obserwacje czasu trwania ruchu plemników. Stwierdzono, że rtęć we wszystkich użytych koncentracjach istotnie obniżyła ruchliwość plemników wyrażoną wszystkimi komputerowo analizowanymi parametrami (VCL, VAP, VSL), mając letalny wpływ w koncentracjach 0,5 oraz 1 ppm. Subiektywne pomiary czasu trwania ruchu plemników wykazały, iż rtęć istotnie obniża czas trwania ruchu plemników, począwszy od koncentracji 0,2 ppm, całkowicie hamując ich ruch w stężeniu 1 ppm. Powyższe wyniki dowodzą negatywnego wpływu rtęci na ruchliwość plemników oraz rozbieżności w dokładności pomiarów obydwu zastosowanych metod analizy ruchliwości plemników.

Słowa kluczowe

EN

fish; carp; mercury; metal; Cyprinus carpio; toxicity; in vitro; common carp; pollutant; sperm motility

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2001
• Tom: 09
• Numer: 1
• Opis fizyczny: p.51-60,fig.

Twórcy

autor

Chyb J

• Agricultural University, 30-199 Krakow, Prof.Spiczakowa 6, Poland

autor

Sokolowska-Mikolajczyk M.

autor

Kime D.E.

autor

Socha M.

autor

Epler P.

Bibliografia

• Allen P. 1994 - Distribution of mercury in the soft tissues of the blue tilapia Oreochromis aureus (Stein- dachner) after acute exposure to mercury (II) chloride - Bull. Environ. Contam. Toxicol. 53: 675 - 683.
• Billard R., Cosson M. P. 1992 - Some problems related to the assessment of sperm motility in freshwater fish -J. Exp. Zool. 261: 122 - 131.
• Billard R., Rubaud P. 1985 - The effect of metals and cyanide on fertilization in rainbow trout (Salmo gairdneri) - Water Res. 19: 209 - 214.
• Centola G. M. 1996 - Comparison of manual microscopic and computer- assisted methods for analysis of sperm count and motility - Arch. Androl. 36: 1 - 7.
• Chyb J., Kime D. E., Mikołajczyk T., Szczerbik P., Epler P. 2000 - The influence of zinc on sperm motility of common carp - a computer assisted studies - Arch. Ryb. Pol. 8: 5 - 14.
• Dey S., Bhattacharya S. 1989 - Ovarian damage to Channa punctatus after chronic exposure to low concentrations elsan, mercury and ammonia - Ecotoxicol. Environ. Safety 17: 247 - 257.
• Ebrahimi M., Nysten K., Roelants I., Ollevier F., Kime D. E. 1996 - Use of computer assisted sperm analysis (CASA) for monitoring sperm quality; application for determining effects of heavy metal pollutants: 47 - 49 - In: Larvi 95 - Fish & Shellfish Larviculture Symposium, European Aquaculture Soc., Special Publication No. 24 (Ed.) P. Lavens, E. Jaspers and I. Roelants, Gent, Belgium.
• Gwoździński K. 1994 - Structural changes of proteins in fish red blood cells after copper and mercury treatment - Arch. Environ. Contam. Toxicol. 23: 426 - 430.
• Heisinger J. F., Green W. 1975 - Mercuric chloride uptake by eggs of the ricefish and resulting teratogenic effects - Bull. Environ. Contam. Toxicol. 14: 665 - 673.
• Joy K. P., Kirubagaran R. 1989 - An immunocytochemical study on the pituitary gonadotropic and thyrotropic cells in catfish, Clarias batrachus after mercury treatment - Biol. Struct. Morphol. 2: 67 - 70.
• Kabata-Pendias A., Pendias H. 1979 - Trace elements in the biological environment - Wyd. Geologiczne, Warszawa, 300 (in Polish).
• Khan A. T., Weis J. S. 198 a - Toxic effects of mercuric chloride on sperm and egg viability of two populations of mummichog, (Fundulus heteroclitus) - Environ. Poll. 48: 263 - 273.
• Khan A. T., Weis J. S. 1987b - Effects of methylmercury on sperm and egg viability of two populations of killifish (Fundulus heteroclitus) - Arch. Environ. Contam. Toxicol. 16: 499 - 505.
• Kime D. E. 1998 - Endocrine disruption in fish - Kluwer Academic Publishers, USA.
• Kime D. E. Ebrahimi M., Nysten K., Roelants I. Moore H. D. M., Ollevier F. 1996 - Use of computer assisted sperm analysis (CASA) for monitoring sperm quality; application for determining effects of heavy metals - Aquatic Toxicol. 36: 223 - 237.
• Kirubagaran R., Joy K. P. 1988 - Toxic effects of mercuric chloride, methylmercuric chloride, and emisan 6 (an organic mercurial fungicide) on ovarian recrudescence in the catfish Clarias batrachus (L.) - Bull. Environ. Contam. Toxicol. 41: 902 - 909.
• Kirubagaran R., Joy K. P. 1992 - Toxic effects of mercury on testicular activity in the freshwater teleost, Clarias batrachus (L.) - J. Fish. Biol. 41: 305 - 315.
• McIntyre J. D. 1973 - Toxicity of methylmercury for steelhead trout sperm - Bull. Environ. Contam. Toxicol. 9: 98 - 99.
• Mohamed M. K., Evans T. C., Mottet N. K., Burbacher T. M. 1986 - Effects of methyl mercury on sperm oxygen consumption - Acta Pharmacol. Toxicol. 58: 219 - 240.
• Nielsen J. B. 1992 - Toxicokinetics of mercuric chloride and methylmercuric chloride in mice - J. Toxicol. Environ. Health. 37: 85 - 122.
• Protasowicki M. 1991 - Long-term studies on heavy metals in aquatic organisms from the river Odra mouth area - Acta Ichthyol. Piscatoria 21: 301 - 311.
• Ram R. J., Sathyanesan A. G. 1984 - Mercuric chloride induced changes in the protein, lipid and cholesterol levels of the liver and ovary of the fish Channa punctatus - Environ. Ecol. 2: 113 - 117.
• Toffelson L., Cordle F. 1986 - Methylmercury in fish: a review on residue levels, fish consumption and regulatory action in the United States - Environ. Health. Persp. 68: 203 - 208.
• Toth G. P., Christ S. A., McCarthy H. W., Torsella J. A., Smith M. K. 1995 - Computer- assisted motion analysis of sperm from the common carp - J. Fish Biol. 47: 986 - 1003.
• Voccia I., Krzystyniak K., Dunier M., Flipo D., Fournier M. 1994 - In vitro mercury-related toxicity and functional impairment of the immune cells of rainbow trout (Oncorhynchus mykiss) - Aquatic Toxicol. 29: 37 - 48.