Genetic mapping and tagging of wheat genes using RAPD, STS and SSR markers

• Autorzy: Khlestkina E K , Pestsova E.G. , Salina E. , Roder M.S. , Arbuzova V.S. , Koval S.F. , Borner A.
• Języki publikacji: EN

Abstrakty

EN

We applied SSR markers for mapping genes determining red coleoptile colour in wheat (Rc1, Rc2, Rc3) using F2 populations. All three genes map at about 15 to 20 cM distally from the centromere of chromosomes 7AS, 7BS and 7DS, respectively. The locations of the glume colour (Bg, Rg1) and glume hairiness (Hg) genes relative to the SSR markers of the homoeologous chromosomes group 1 were determined using molecular analysis of near-isogenic lines (NILs). One RAPD marker for the vernalisation response gene Vrn-A1 was identified by screening 95 random primers against two pairs of NILs. New PCR (STS) markers were developed based on RFLP-markers PSR426 (5A, 5B, 5D) and PSR1201 (1A, 5A, 5B). Analysis of nulli-tetrasomic and near-isogenic lines of wheat using the STS markers developed gave an indication that these new STS markers have the same chromosomal and intrachromosomal positions as the correspondent RFLP markers. Therefore, they could be used for mapping and/or tagging the vernalisation response (Vrn-A1, Vrn-B1, Vrn-D1) and homoeologous pairing (Ph1) genes.

Słowa kluczowe

EN

glume colour; vernalization response; gene; random amplified polymorphic DNA; wheat; plant genetics; Triticum aestivum; genetic mapping; molecular marker; F2 population; red coleoptile colour

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.795-802,fig.

Twórcy

autor

Khlestkina E K

• Russian Academy of Sciences, Novosibirsk, 630090, Russia

autor

Pestsova E.G.

autor

Salina E.

autor

Roder M.S.

autor

Arbuzova V.S.

autor

Koval S.F.

autor

Borner A.

Bibliografia

• 1. McIntosh, R. A., Hart, G. E., Devos, K. M., Gale, M. D. and Rogers, W. J. Catalogue of gene symbols for wheat. In: Proc 9th Int Wheat Genet. Symp. (Slinkard, A. E. Ed.), vol. 5, 1998, pp. 1-236. University Extension Press, University of Saskatchewan.
• 2. McIntosh, R. A., Devos, K. M., Dubcovsky, J. and Rogers, W. J. Catalogue of gene symbols for wheat: 1999 Supplement. Wheat Information Service 89 (1999) 37-85.
• 3. McIntosh, R. A., Devos, K. M., Dubcovsky, J. and Rogers, W. J. Catalogue of gene symbols for wheat: 2000 Supplement. Wheat Information Service 91 (2000) 33-70.
• 4. McIntosh, R. A., Devos, K. M., Dubcovsky, J. and Rogers, W. J. Catalogue of gene symbols for wheat: 2001 Supplement. Wheat Information Service 93 (2001) 40-60.
• 5. Röder, M. S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M. H., Leroy, P. and Ganal, M. W. A microsatellite map of wheat. Genetics 149 (1998) 2007-2023.
• 6. Muehlbauer, G. J., Specht, J. E., Thomas-Compton M. A., Staswick P. E. and Bernard, R. L. Near-isogenic lines - a potential resource in the integration of conventional and molecular marker linkage maps. Crop Sci. 28 (1988) 729-735.
• 7. Khlestkina, E. K., Salina, E. A., Pshenichnikova, T. A., Arbuzova, V. S. and Koval, S. F. Analysis of Near-isogenic lines of common wheat carrying the dominant alleles of Hg, Hg and Rgl genes using microsatellite and protein markers. Russ. J. Genet. 36 (2000) 1153-1158.
• 8. Khlestkina, E. K., Salina, E. A., Pshenichnikova, T. A., Arbuzova, V. S. and Koval, S. V. The use of RAPD and STS analyses for marking genes of homoeologous group 5 chromosomes of common wheat. Russ. J. Genet. 35 (1999) 1161-1168.
• 9. Sharp, P. J., Chao, S., Desai S. and Gail M. D. The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor. Appl. Genet. 78 (1989) 342-348.
• 10. Plaschke, J., Börner, A., Wendehake, K., Ganal, M. W. and Röder, M. S. The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 82 (1996) 33-40.
• 11. Plaschke, J., Ganal, M. W. and Röder, M. S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91 (1995) 1001-1007.
• 12. Lander, E. S., Green, P., Abrahamson, J., Barlow, A., Daly, M. J., Lincoln, S. E. and Newburg, I. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1 (1987) 174-181.
• 13. Xie, D. X., Devos, K. M., Moore, G. and Gale, M. D. RFLP-based genetic maps of homeologous group 5 chromosomes of bread wheat (Triticum aestivum L.), Theor. Appl. Genet. 87 (1993) 70-74.
• 14. Sanger, F., Nicklen, S. and Coulson, A. R. DNA Sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sci. 74 (1977) 5463-5476.
• 15. Chao, S., Sharp, P. J., Worland, A. J., Warham, E. J., Koebner, R. M. D. and Gale, M. D. RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor. Appl. Genet. 78 (1989) 495-504.
• 16. Nelson, J. C., Sorrels, M. E., Van Deynze, A. E., Lu, Y. H. Atkinson, M., Bernard, M., Leroy, P., Faris, J. D. and Anderson, J. A. Molecular mapping of wheat: Major genes and rearrangements in homoeologous groups 4, 5, and 7. Genetics 141 (1995) 721-731.
• 17. Khlestkina, E. K., Pestsova, E. G., Röder, M. S. and Börner, A. Molecular mapping, phenotypic expression and geographical distribution of genes determining anthocyanin pigmentation of coleoptiles in wheat (Triticum aestivum L.). Theor. Appl. Genet. (2002), in press.
• 18. Malyshev, S., Korzun, V., Voylokov, A., Smirnov, V. and Börner, A. Linkage mapping of mutant loci in rye (Secale cereale L.). Theor. Appl. Genet. 103 (2001) 70-74.
• 19. Franckowiak, J. D. Revised linkage maps for morphological markers in barley, Hordeum vulgare. Barley Genet. Newslett. 26 (1997) 9-21.
• 20. Lundqvist, U., Franckowiak, J. D. and Konishi, T. New and revised descriptions of barley genes. Barley Genet. Newslett. 26 (1997) 22-516.
• 21. Börner, A., Schumann, E., Fürste, A., Cöster, H., Leithold, B., Röder, M. S. and Weber, W. E. Mapping of quantitative trait loci for agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor. Appl. Genet. (2002), in press.
• 22. Talbert, L. E., Blake, N. K., Chee, P. W., Blake, T. K. and Magyar, G. M. Evaluation of Sequence-Tagged-Site PCR Products as Molecular Markers in Wheat. Theor. Appl. Genet. 87 (1994) 789-794.
• 23. Erpelding, J. E., Blake, N. K., Blake, T. K. and Talbert, L. E. Transfer of Sequence Tagged Site PCR Markers between Wheat and Barley. Genome 39 (1996) 802-810.
• 24. Clarke, B., Stancombe, P., Money, T., Foote, T. and Moore, G. Targeting Deletion (Homeologous Chromosome Pairing Locus) or Addition Line Single Copy Sequences from Cereal Genomes. Nucleic Acids Res., 20 (1992) 1289-1292.
• 25. Galiba, G., Quarrie, S. A., Sutka, J., Morgunov, A. and Snape, J. W. RFLP mapping of the vernalisation (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat. Theor. Appl. Genet. 90 (1995) 1174-1179.