Plant regeneration from immature inflorescence-derived callus of Italian ryegrass [Lolium multiflorum Lam.]: chromosome number and fertility of regenerated plants

• Autorzy: Zwierzykowski Z , Rybczynski J.J.
• Języki publikacji: EN

Abstrakty

EN

Twenty field-grown genotypes of diploid Italian ryegrass (Lolium multiflorum Lam., 2n = 2x = 14) were tested for their ability to induce callus and regenerate plants. Callus cultures were initiated from segments of immature inflorescences cultured on the MS medium supplemented with 4.0 mg L⁻¹ 2,4-D. The calluses were subcultured first on the maintaining medium (MS medium with 2.0 mg L⁻¹ 2,4-D) and later on the rooting medium (MS medium with 0.2 mg L⁻¹ 2,4-D). The frequency of callus induction varied depending on the source of explant and the initial genotype. A total of 473 green plantlets were regenerated, of which 420 were established in the soil. All these plants had the morphological characteristics of Italian ryegrass. Among 372 regenerants analysed cytologically, 302 (81.2%) had the expected diploid chromosome number (2n = 2x= 14), 65 (17.5%) were tetraploid (2n = 4x = 28); several aneuploids and mixoploids were also observed. All diploid and tetraploid regenerants were male and female fertile. However, a great variation of female fertility within and between both groups of regenerants was observed.

Słowa kluczowe

EN

chromosome structure; plant regeneration; female fertility; callus culture; chromosome number; meiotic behaviour; Italian rye-grass; breeding method; callus induction; plant breeding; Lolium multiflorum; immature inflorescence

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1998
• Tom: 39
• Numer: 3
• Opis fizyczny: p.217-224

Twórcy

autor

Zwierzykowski Z

• Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Rybczynski J.J.

Bibliografia

• Ahloowalia B.S. (1983). Spectrum of variation in somaclones of triploid ryegrass. Crop Sci. 23: 1141-1147.
• Creemers-Molenaar J., Loeffen J.P.M., van der Valk P. (1988). The effect of 2,4 dichlorophenoxyacetic acid and donor plant environment on plant regeneration from immature inflorescence-derived callus of Lolium perenne L. and Lolium multiflorum Lam. Plant Sci. 57: 165-172.
• Dale P.J., Dalton S.J. (1983). Immature inflorescence culture in Lolium, Festuca, Phleum and Dactylis. Z. Pflanzenphysiol. 111: 39-45.
• Dale P.J., Thomas E., Brettell R.I.S., Wernicke W. (1981). Embryogenesis from cultured immature inflorescences and nodes of Lolium multiflorum. Plant Cell Tissue Organ Culture 1: 47-55.
• Eizenga G.C., Dahleen L.S. (1990). Callus production, regeneration and evaluation of plants from cultured inflorescences of tall fescue (Festuca arundinacea Schreb.). Plant Cell Tissue Organ Culture 22: 7-15.
• Garcia A., Dalton S.J., Humphreys M.O. (1994). Reproductive disturbances and phosphoglucoisomerase instability in Festuca arundinacea (tall fescue) plants regenerated from callus and cell suspension cultures. Heredity 73: 355-362.
• Jackson J.A., Dale P.J. (1988). Callus induction, plant regeneration and an assessment of cytological variation in regenerated plants of Lolium multiflorum Lam. J. Plant Physiol. 132: 351-355.
• Jackson J.A., Dale P.J. (1989). Somaclonal variation in Lolium multiflorum Lam. and L. temulentum L. Plant Cell Reports 8: 161-164.
• Kasperbauer M.J., Buckner R.C., Bush L.P. (1979). Tissue culture of annual ryegrass x tall fescue F₁ hybrids: callus establishment and plant regeneration. Crop Sci. 19: 457-460.
• MurashigeT., Skoog F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
• Rajoelina S.R., Alibert G., Planchon C. (1990). Continuous plant regeneration from established embryogenic cell suspension cultures of Italian ryegrass and tall fescue. Plant Breeding 104: 265-271.
• Rybczyński J.J., Zwierzykowski Z., Ślusarkiewicz-Jarzina A. (1983). Plant regeneration with doubled chromosome number in tissue culture of F₁ Lolium-Festuca hybrids. Genet. Pol. 24: 1-8.
• Zwierzykowski Z., Rybczyński J.J. (1994). Effectiveness of tissue culture method for chromosome doubling in sterile Lolium-Festuca hybrids. Genet. Pol. 35A: 73-78.
• Zwierzykowski Z., Ślusarkiewicz-Jarzina A., Rybczyński J.J. (1985). Regeneration of plants and chromosome doubling in callus culture of Lolium multiflorum Lam. (2n=14) x Festuca pratensis Huds. (2n=14) hybrid. Genet. Pol. 26: 187-195.