Polymorphism in the ITS region of ribosomal DNA of Cochliobolus sativus isolates differing in xylanase production

• Autorzy: Bakri Y , Jawhar M. , Arabi M.I.E.
• Języki publikacji: EN

Abstrakty

EN

The restriction of PCR-amplifïed internal transcribed spacers (ITS) of ribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was observed the resolution between clustering of isolates and their xylanase production level.

Słowa kluczowe

EN

xylanase production; internal transcribed spacer; Cochliobolus sativus; genetic diversity; isolate; ribosomal DNA; genetic variation; diversity level

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 1
• Opis fizyczny: p.77-79,fig.,ref.

Twórcy

autor

Bakri Y

• Department of Molecular Biology and Biotechnology, AECS, P.O.Box 6091, Damascus, Syria

autor

Jawhar M.

autor

Arabi M.I.E.

Bibliografia

• Abacus concepts, 1996. Statview 4.5 statistical program. Abacus Concepts Corporation, Berkeley, CA, USA.
• Arabi M. I. E. and M. Jawhar. 2003. Pathotypes of Cochliobolus sativus (spot blotch) on barley in Syria. J. Plant Pathol. 85: 193-196.
• Arabi M.I.E. and M. Jawhar. 2004. Identification of Cochliobolus sativus (spot blotch) isolates expressing differential virulence on barley genotypes in Syria. J. Phytopathol. 152: 461-464.
• Bailey M.J., P. Bailey and R. Poutanen. 1992. Interlaboratory testing of methods for assay of xylanase activity. J. Biotech. 23: 257-270.
• Buchert J., M. Tenkanen, A. Kantelinen and L. Vilkari. 1994. Application of xylanase in the pulp and paper industry. Bioresource Tech. 50: 65-72.
• Farmer D.J. and D.M. Sylvia. 1998. Variation in the ribosomal DNA internal transcribed spacer of a diverse collection of ectomycorrhizal fungi. Mycol. Res. 74: 859-865.
• Geimba M. P., A. Riffel, V. Agostini and A. Bandelli. 1999. Characterization of cellulose-hydrolysing enzymes from the fan-gus Bipolaris sorokiniana. J. Sci. Food Agric. 79: 1849-1854.
• Geln M., EC. Tommerup, N.L. Bougher and P.A. O'Brien. 2001. Interspecific and intraspecific variation of ectomycorrhizal fungi associated with Eucalyptus ecosystems as revealed by ribosomal DNA PCR-RFLP. Mycol. Res. 105: 843-858.
• Hsiang T. and C. Wu. 2000. Genetic relationships of pathogenic Typhula species assessed by RAPD, ITS-RFLP and ITS sequencing. Mycol. Res. 104: 16-22
• Leach J., D.B. Finklstein and J.A. Ramosek. 1986. Rapid miniprep of DNA from filamentous fungi. Fungal Genet. Newsl. 33: 32-33.
• Martin K.J. and P.T. Rygiewicz.2005. Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts. BMC Microb. 5: 21-28.
• Miller G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426-428.
• Nei M. and W.H Li. 1979. Mathematical model for studying genetic variation in terms of restricting endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273.
• Parry D.W., P. Jenkinscn and L. Mcleod. 1995. Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathol. 44: 207-238.
• Peltonen S. 1995. Comparison of xylanase production by fungal pathogens of barley with special reference to Bipolaris sorokiniana. Mycol. Res. 6: 717-723.
• Redecker D., H. Thierfelder, C. Walker and D. Werner. 1997. Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order Glomales. Appl. Environ. Microb. 63: 1756-1761.
• Singh S., B. Pillay and B.A. Prior. 2003. Thermal stability of β-xylanase produced by different Thermomyces lanuginosus strains. Enzyme Microb. Technol. 26: 502-208.
• Statsoft 2003. STATISTICA, data analysis software system, 6th ver.' Available at_www.statsoft.com (verified 25 October 2006).
• White T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand D.H., Sninsky J.J. White T.J. (eds), PCR Protocols; a Quide to Methods and Applications, pp. 315-322. Academic Press, San Diego, CA, USA.
• Wong K.K.Y., C. S. James and S.H. Campion. 2000. Xylanase pre and post-treatments of bleached pulps decrease absorption coefficient. J. Pulp Pap. Sci. 26: 377-383.