Wystepowanie w latach 2002-2004 szczepow Staphylococcus aureus z fenotypem opornosci MLSB w probkach materialu klinicznego

• Autorzy: Kaczmarek A. , Budzynska A. , Gospodarek E.

Warianty tytułu

EN

Occurrence of macrolide-lincosamide-streptogramin B resistant isolates of Staphylococcus aureus in clinical specimens in 2003-2004

• Języki publikacji: PL

Abstrakty

PL

Oceniono częstość występowania oporności typu MLSB wśród szczepów MSSA (n=1682) i MRSA (n=272) izolowanych z próbek materiału klinicznego w latach 2002-2004 w Szpitalu Uniwersyteckim im. A. Jurasza CM w Bydgoszczy UMK w Toruniu. W kolejnych latach szczepy S. aureus MLSB+ stanowiły w populacji MSSA 3,1%, 3,2%, 3,8%, natomiast w populacji MRSA- 22,8%, 25,4% i 19,8%.

EN

The aim of the study was the analysis of frequency of macrolide-lincosamide-streptogramin В (MLSB) resistance among MSSA (n=1682) and MRSA (n=272) strains which were isolated in 2002- 2004 from various clinical materials from patients hospitalized in the University Hospital at the L. Rydygier Collegium Medicum in Bydgoszcz, University of Nicolaus Copernicus in Toruń. Susceptibility testing and examination of methicillin-resistant strains were performed by the disc diffusion techniques according to recommendation of NCCLS. Resistance to the MLSB antimicrobials agents was higher among MRSA compared to MSSA isolates. The MLSB constitutive phenotype was more prevalent than the MLSB inducible phenotype among investigated MRSA (65,4%) and MSSA (7,6%) isolates. Inducible resistance had only 2,5% of the MSSA and 2,6% of the MRSA strains. Moreover in 2004 there were found increasing frequency of inducible MLSB resistance from 1,1% to 5,7% and decreasing frequency of constitutive MLSB resistance from 9,2% to 4,7% among MSSA strains, in comparison to 2003. The investigated MSSA MLSB+, MLSB and MRSA MLSB+, MLSB- strains were the most frequently isolated from pus (adequately 5,2%, 28,8% and 30,5%, 10,7%) and also from nosopharynx swabs ( 1,7% MSSA MLSB+ and 22,9% MSSA MLSB-) and biomaterials ( 15,1 % MRSA MLSB+ and 9,6% MRSA MLSB-). They mainly came from patients of the outpatient clinic (2,4% MSSA MLSB+ and 19,9% MSSA MLSB- and patients treated at the neurosurgical ward (20,6% MRSA MLSB+ and 12,1% MRSA MLSB- ).

Słowa kluczowe

PL

zakazenia szpitalne; szczepy bakteryjne; wystepowanie; lata 2002-2004; Staphylococcus aureus; lekoopornosc; metycylina

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 2
• Opis fizyczny: s.103-111,rys.,bibliogr.

Twórcy

autor

Kaczmarek A.

• Collegium Medicum im.L.Rydygiera w Bydgoszczy Uniwersytetu Mikolaja Kopernika w Toruniu, ul.M.Sklodowskiej-Curie 9, 85-094 Bydgoszcz

autor

Budzynska A.

autor

Gospodarek E.

Bibliografia

• 1. Almer LS, Shortridge VD, Nilius AM i inni. Antimicrobial susceptibility and molecular chracterization of community-acquired methicillin-resistant Staphylococcus aureus. Diagn Microbiol Infect Dis 2002; 43: 225-32.
• 2. Dzierżanowska D. Antybiotykoterapia praktyczna, á-medica press. Wydanie III. Bielsko-Biała 2001.
• 3. Fiebelkorn KR, Crawford SA, McElmeel ML i inni. Practical disk diffusion method for detection of inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci. J Clin Microbiol 2003; 41: 4740-4.
• 4. Hamilton-Miller JM, Shah S. Patterns of phenotypic resistance to the macrolide-lincosamide-ketolide-streptogramin group of antibiotics in staphylococci. J Antimicrob Chemother 2000; 46: 941-9.
• 5. Hryniewicz W, Sidikowska A, Szczypa K i inni. Rekomendacje doboru testów do oznaczania wrażliwości bakterii na antybiotyki i chemioterapeutyki. Mikrobiol Med 2004; 3: 3-28.
• 6. Inoue Y, Morifuji K, Masumi N i inni. Susceptibility of methicillin-resistant Staphylococcus aureus clinical isolates to various antimicrobial agents. III. Novel, inducible resistance to macrolide-lincosamide-streptogramin В (MLS) antibiotics. Hiroshima J Med. Sci 1993; 42:101-7.
• 7. Kim HB, Lee В, Jang HC i inni. A high frequency of macrolide-lincosamide-streptogramin resistance determinants in Staphylococcus aureus isolated in South Korea. Microb Drug Resist 2004; 10: 248-54.
• 8. Lina G, Quaglia A, Reverdy ME i inni. Distrubution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci. Antimicrob Agents Chemother 1999; 43: 1062-6.
• 9. Marr JK, Lim AT, Yamamoto LG. Erythromycin-induced resistance to clindamycin m Staphylococcus aureus. Hawaii Med. J 2005; 64: 6-8.
• 10. Młynarczyk A, Młynarczyk G, Jeljaszewicz J. Lokalizacja genów oporności na wybrane grupy antybiotyków antybiotyków metycylinoopornych szczepów Staphylococcus aureus. Med Dośw Mikrobiol 1999; 51: 9-16.
• 11. Młynarczyk A, Młynarczyk G, Łuczak M i inni. Oporność na antybiotyki szczepów Staphylococcus aureus izolowanych w różnych szpitalach warszawskich. Med Dośw Mikrobiol 2001; 53: 217-25.
• 12. Młynarczyk A, Młynarczyk G, Łuczak M. Mechanizmy oporności na antybiotyki szczepów MRSA (metycylinooporne Staphylococcus aureus). Zakażenia 2004; 6: 26-30.
• 13. Młynarczyk G. Molekularne podstawy antybiotykooporności bakterii. Przegl Epidemiol 2000; 54 (supi. 1): 18-25.
• 14. NCCLS (National Committe for Clinical Laboratory Standards). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobinally. Approved standard 6th edition 2003, M7- A6, Wayne PA.
• 15. Nicola FG, McDougal LK, Biddle JW i inni. Characterization of erythromycin-resistant isolates of Staphylococcus aureus recovered in the United States from 1958 through 1969. Antimicrob Agents Chemother 1998; 42: 3024-7.
• 16. Roberts MC, Sutcliffe J, Courvalin P i inni. Nomenclature for macrolide-lincosamide-streptogramin В resistance determinants. Antimicrob Agents Chemother 1999; 43: 2823-30.
• 17. Schmitz HJ, Sadurski R, Kray A. Prevalence of macrolide-resistance genes in Staphylococcus aureus and Enterococcus faecium isolates from 24 European university hospitals. J Antimicrob Chemother 2000; 45: 891-4.
• 18. Seal JB, Moreira В, Bethel CD i inni. Antimicrobial resistance in Staphylococcus aureus at the University of Chicago Hospitals: a 15-year longitudinal assessment in a large university-based hospital. Infect Control Hosp Epidemiol 2003; 24: 403-8.
• 19. Thakker-Varia S, Jenssen WD, Moon-McDermott L. Molecular epidemiology of macrolides-lincosamides-streptogramin В resistance in Staphylococcus aureus and coagulase-negative staphylococci. Antimicrob Agents Chemother 1987; 31: 735-43.
• 20. Jester B, Douthwaite S. Macrolide resistance conferred by base substitutions in 23S rRNA. Antimicrob Agents Chemother 2001; 45: 1-12.