Evaluation of anti-gout activity of some plant food extracts
Treść / Zawartość
The anti-gout activity of methanol and petroleum ether extracts of celery leaves, celery seeds, rosemary, cinnamon and turmeric as functional food components was studied in potassium oxonate treated rats (250 mg/kg body weight, intra-peritoneal). Blood samples were collected from all rats after an overnight fast and after 3 and 6 h from oxonate injection for determination of erythrocyte sedimentation rate (ESR), plasma uric acid, nitric oxide (NO) and malondialdehyde (MDA). Urine samples were collected for 6 h after injection for the determination of uric acid. Assessment of total phenolic contents, fatty acids and unsaponifiable matter (UNSAP) in the plants under study was carried out. Results showed that oxonate treatment produced a significant increase in all studied parameters compared to the healthy rats. Oral administration of different extracts (500 mg/kg body weight) showed a significant reduction in plasma and urine uric acid levels, petroleum ether extract of celery seeds was the most promising. The majority of administered extracts showed significant reduction in inflammatory (ESR and NO) and oxidative stress (MDA) markers with variable degrees. GLC investigation of plants UNSAP revealed the presence of different phytosterols. GLC analysis of the fatty acids methyl ester showed that celery seeds and leaves contained the highest contents of oleic and linoleic acid, respectively. Linolenic acid was only present in celery seeds and leaves. All the studied plants were rich in phenolics; rosemary was superior in this respect. In conclusion, the studied plant extracts showed significantly variable anti-gout activity associated with both antioxidant and antiinflammatory effects, which may be due to the presence of phenolic compounds, unsaturated fatty acids, long chain fatty acids and phytosterols.
- 1. Almela L., Sanchez-Munoz B., Fernandez-Lopez J.A., Roca M.J., Rabe V., Liquid chromatographic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material. J. Chromatogr., 2006, 1120, 221–229.
- 2. A.O.A.C. Official Methods of Analysis of the Association of Official Agricultural Chemists, 2000, 17th ed, Published by A.O.A.C.
- 3. Beneke M., Avoid purine-rich foods, drink a lot, reduce weight. The most important recipes against hyperuricemia: MMW Fortsch. Med., 2003, 145, 36–45.
- 4. Bouic P.J., Lamprecht J.H., Plant sterols and sterolins: a review of their immune-modulating properties. Altern. Med. Rev., 1999, 4, 170–177.
- 5. Bouic P.J., The role of phytosterols and phytosterolins in immune modulation: a review of the past 10 years. Curr. Opin. Clin. Nutr. Metab. Care, 2001, 4, 471–475.
- 6. Chan M.M., Ho C.T., Hung H.I., Effects of three dietary phytochemicals from tea, rosemary and turmeric on inflammation‑induced nitrite production. Cancer Lett., 1995, 96, 23–29.
- 7. Chen W.F., Deng S.L., Zhou B., Yang L., Liu Z.L., Curcumin and its analogues as potent inhibitors of low density lipoprotein oxidation: H-atom abstraction from the phenolic groups and possible involvement of the 4-hydroxy-3-methoxyphenyl groups. Free Radic. Biol. Med., 2006, 40, 526–35.
- 8. Chiang H.C., Lo Y.J., Lu F.J., Xanthine oxidase inhibitors from the leaves of Alsophila spinulosa (Hook) Tryon. J. Enzyme Inhib., 1994, 8, 61–71.
- 9. Corrado A., D’Onofrio F., Santoro N., Melillo N., Cantore F.P., Pathogenesis, clinical findings and management of acute and chronic gout. Minerva Med., 2006, 97, 495–509.
- 10. Dalbeth N., Haskard D.O., Mechanisms of inflammation in gout. Rheumatology (Oxford), 2005, 44, 1090–1096.
- 11. Deodhar S.D., Sthi R., Srimal R.C., Preliminary study on antirheumatic activity of curcumin (diferuloylmethane). Indian J. Med. Res., 1980, 71, 632–634.
- 12. Dillard C.J., German J.B., Phytochemicals and human health. J. Sci. Food Agric., 2000, 80, 1744–1756.
- 13. Duthie G.G., Duthie S.J., Kyle J.A.M., Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutr. Res. Rev., 2000, 13, 79–106.
- 14. Golding D.N., A Synopsis of Rheumatic Diseases, 1989, 5th ed., Page bros, Ltd, Norwich, England p. 156.
- 15. Gomez M.A., Saenz M.T, Garcia M.D., Fernandez M.A., Study of the topical anti-inflammatory activity of Achillea ageratum on chronic and acute inflammation models. Z. Naturforsch. [C], 1999, 54, 937–941.
- 16. Joe B., Lokesh B.R., Role of capsaicin, curcumin and dietary n-3 fatty acids in lowering the generation of reactive oxygen species in rat peritoneal macrophages. Biochin. Biophys. Acta., 1994, 1224, 255–263.
- 17. Kong L.D., Cai Y., Huang W.W., Cheng C.H., Tan R.X., Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout. J. Ethnopharmacol., 2000, 73, 199–207.
- 18. Lee J.S., Jeon S.M., Park E.M., Huh T.L., Kwon O.S., Lee M.K., Choi M.S., Cinnamate upplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterolfed rats. J. Me. Food, 2003, 6, 183–191.
- 19. Lee J.J., Jin Y.R., Lim Y., Hong J.T., Kim T.J., Chung J.H., Yun Y.P., Antiplatelet activity of carnosol is mediated by the inhibition of TXA2 receptor and cytosolic calcium mobilization. Ascul Pharmacol., 2006, 45, 148–153.
- 20. Lo A., Liang Y., Lin-Shiau S., Ho C., Lin J., Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating nuclear factor-κB in mouse macrophages. Carcinogenesis, 2002, 23, 983–991.
- 21. Löliger J., The use of antioxidants in food. 1991, in: Free Radicals and Food Additives (eds. O.I. Aruoma, B. Halliwell). Taylor and Francis, London, pp. 129–138.
- 22. Mohamed D.A., Ismael A.I., Ibrahim A.R., Studying the anti-inflammatory and biochemical effects of wheat germ oil. Deutsche Lebensmittel-Rundschau, 2005, 101, 66–72.
- 23. Mohamed D.A., Al-Okbi S.Y., Xanthine oxidase inhibition and antioxidant activity of some plant foods (in press).
- 24. Momin R.A., Nair M.G., Antioxidant, cyclooxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. seeds. Phytomedicine, 2002, 9, 312–318.
- 25. Montgomery H.A.C., Dymock J.F., The determination of nitrite in water. Analyst, 1961, 86, 414–416.
- 26. Nuki G., Simkin P.A., A concise history of gout and hyperuricemia and their treatment. Arthritis Res. Ther., 2006, 8 (Suppl. 1), S1-S5.
- 27. Owen P.L., Johns T., Xanthine oxidase inhibitory activity of northeastern North American plant remedies used for gout. J. Ethnopharmacol., 1999, 64, 149–60.
- 28. Popovic M., Kaurinovic B., Trivic S., Mimica-Dukic N., Burac M., Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytother Res., 2006, 20, 531–537.
- 29. Rahman I., Biswas S.K., Kirkham P.A., Regulation of inflammation and redox signaling by dietary polyphenols. Biochem. Pharmacol., 2006, 72, 1439–1452.
- 30. Ranjbar A., Ghasmeinezhad S., Zamani H., Malekirad A.A., Baiaty A., Mohammadirad A., Abdollahi M., Antioxidative stress potential of Cinnamomum zeylanicum in humans: a comparative cross-sectional clinical study. Therapy, 2006, 3, 113–117.
- 31. Saso L., Valentini G., Casini M.L., Mattei E., Braghiroli L., Mazzanti G., Panzironi C., Grippa E., Silvestrini B., Inhibition of protein denaturation by fatty acids, bile salts and other natural substances: a new hypothesis for the mechanism of action of fish oil in rheumatic diseases. Jpn. J. Pharmacol., 1999, 79, 89–99.
- 32. Satoh K., Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta, 1978, 20, 37–43.
- 33. Singleton V.L., Rossi J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 1965, 16, 144–158.
- 34. Solomon A., Golubowicz S., Yablowicz Z., Grossman S., Bergman M., Gottlieb H.E., Altman A., Kerem Z., Flaishman M.A., Antioxidant activities and anthocyanin content of fresh fruits of common fig (Ficus carica L.). J. Agric. Food Chem., 2006, 54, 7717–7723.
- 35. Stein H.B., Hasan A., Fox I.H., Ascorbic acid-induced uricosuria. A consequency of megavitamin therapy. Ann. Intern. Med., 1976, 84), 385–388.
- 36. Surh Y.N., Chun K.S., Cha H.H., Han S.S., Keum Y.S., Park K.K., Lee S.S., Molecular mechanism underlying chemopreventive activities of anti-inflammatory phytochemicals: down regulation of COX-2and iNOS through suppression of F-κB activation. Mutat. Res., 2001, 480–481, 243–268.
- 37. Unno T., Sugimoto A., Kakuda T., Xanthine oxidase inhibitors from the leaves of Lagerstroemia speciosa (L.) Pers. J. Ethnopharmacol., 2004, 93, 391–395.
- 38. Urano W., Yamanaka H., Tsutani H., Nakajima H., Matsuda Y., Taniguchi A., Hara M., Kamatani N., The inflammatory process in the mechanism of decreased serum uric acid concentrations during acute gouty arthritis. J. Rheumatol., 2002, 29, 1950–1953.
- 39. Wang T., Hicks K.B., Moreau R., Antioxidant activity of phytosterols, oryzanol, and other phytosterol conjugates. J. Am. Oil Chem. Soc., 2002, 79, 1201–1206.
- 40. Watts R.W.E., Determination of uric acid in blood and urine. Ann. Clin. Biochem., 1974, 11, 103–111.
- 41. Westergren A., Studies of the suspension stability of the blood in pulmonary tuberculosis. Acta Medica Scandinavica., 1921, 54, 247–245.
- 42. Wood J.A., Jewell C., O’Brien N.M., Sedanolide, a natural phthaide from celery seed oil: effect on hydrogen peroxide and tert-butyl hydroperoxide-induced toxicity in HepG2 and CaCo-2 human cell lines. Int. Vitr. Mol. Toxicol., 2001, 14, 233–240.
- 43. Yegnanarayan R., Saraf A.P., Balwani J.H., Composition of antiinflammatory activity of various extract of Curcuma longa (Linn). Indian J. Med. Res., 1976, 64, 601–608.
- 44. Yonetani Y., Iwaki K., Ogawa Y., Decreasing effect of allantoxanamide, a hypuricemic agent on renal functions in rats. Jpn. J. Pharmacol., 1987, 45, 37–43.