Wstepne badania czynnika etiologicznego ludzkiej ehrlichiozy [HGE] w kleszczach z zachodniopolnocnej Polski

• Autorzy: Skotarczak B , Rymaszewska A.
• Języki publikacji: PL

Abstrakty

EN

Ehrlichias occur in ticks in the cells of their haemolymph-hematocytes. They enter the vertebrate host organism with the saliva of the tick, during a blood meal. Humans can also be the hosts for this pathogen. Two pathogens cause a humane disease-monocytic ehrlichiasis (E. chaffensis) or granulocytic ehrlichiasis (HGE factor). The above disease units are difficult to diagnose because of their non-specific symptoms. A preliminary study has been conducted on the prevalence of the HGE factor in the ticks, Ixodes ricinus in the recreational areas of the West-Pomeranian Province. All forms of I. ricinus were collected from 3 sites. All the sites are known to be frequented by hikers and gatherers of forest mushrooms and berries. The site selection involved also careful consideration of the tree- and underbrush type. The ticks were collected twice a year in spring (May/June) and in autumn (August\September), which was associated with the biological activity of the collected acarines. A total of 1159 Ixodes ricinus ticks were collected, in this number 172 females, 167 males, 597 nymphs, and 223 larvae. Using the PCR technique, the 16SrRNA-gene fragment was amplified using primers specific for the HGE factor: EHR 790 and EHR 521. The studied population contained 3.7% infected females in spring and 2.7% in autumn, 0.68% infected males in spring, no infected in autumn. The nymphs were infected in spring (2.17%) and in autumn too (0.73%), but the larvae were not infected in both seasons. Analysing the above-mentioned results it can be concluded that the decisive majority of the individuals transmitting the HGE factor are the adult forms.The present study was only a preliminary one. In the future much more sites will be monitored, in the recreational areas of both the city of Szczecin and the entire province.

Słowa kluczowe

PL

wektory chorob; czynniki chorobotworcze; Polska Zachodnio-Polnocna; choroby czlowieka; kleszcze; parazytologia; etiologia; erlichioza; Ehrlichia

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 1
• Opis fizyczny: s.95-101,tab.,bibliogr.

Twórcy

autor

Skotarczak B

• Uniwersytet Szczecinski, ul.Lukasinskiego 43, 71-215 Szczecin

autor

Rymaszewska A.

Bibliografia

• ANDERSON B. E., DAWSON J. E., JONES D. C., WILSON K.H. 1991. Ehrlichia chaffeensis, a new species associated with human ehrliehiosis. J. Clin. Microbiol. 29 (12): 2838-2842.
• ANDERSON B. E., SUMNER J. W., DAWSON J. E., TZIANABOS T., GREENE C. R., OLSON J. G., FISHBEIN D. B., OLSEN-RASUSSEN M., HOLLOWAY B. P., GEORGE E. H., AZAD A. F. 1992. Detection of the etiologic agent of human ehrliehiosis by polymerase chain reaetion. J. Clin. Microbiol. 30 (4): 775-780.
• ANDERSON B. E., SIMS K. G., OLSON J. G., CHILDS J. E., PIESMAN J. F., HAPP C. M., MAUPIN G. O., JOHNSON B. J. B. 1993. Amblyomma americanum: a potential vector of human ehrliehiosis. Am. J. Trop. Med Hyg. 49 (2): 239-244.
• BAJER A., BEHNKE J. M., PAWELCZYK A., SIŃSKI E. 1999. First evidence of Ehrlichia sp. in wild Microtus arvalis from Poland. Acta Parasitol. 44 (3): 204-205.
• BAKKEN I. S., KRUETH J., WILSON-NORDSKOG C., TILDEN R. L., ASANOVICH K., DUMLER J. S. 1996. Clinieal and laboratory characteristies of human granulocytie ehrliehiosis. JAMA. 215(3): 199-202.
• CHEN S. M., DUMLER J. S, BAKKEN J. S., WALKER D. H. 1994. Identification of a granulocytoyropie Ehrlichia species as the etiologie agent of human disease. J. Clin. Microbiol. 32 (3): 589-595.
• CHEN S. M., DUMLER J. S., FENG H. M., WALKER D. H. 1994a Identification of the antigenetie constituents of Ehrlichia chaffeensis. Am. J. Trop. Med. Hyg. 50(1): 52-58.
• CHRISTOVA I. S., DUMLER J. S. 1999. Human granulocytic ehrliehiosis in Bulgaria. Am. J. Trop. Med. Hyg. 60 (1): 58-61.
• DAWSON J. E., ANDERSON B. E., FISHBEIN D. B., SANCHEZJ. L., GOLDSMITH C. S., WILSON K. H., DUNTLEY C. W. 1991. Isolation and characterization ofan Ehrlichia sp. from a patient diagnosed with human ehrliehiosis. J. Clin. Microbiol. 29(12): 2741-2745.
• DUMLER J. S., BAKKEN J. S. 1995. Ehrliehial diseases of humans: emerging tick borne infections. Clin. Infect. Dis. 20: 1102-1110.
• EVEREIT E. D., EVANS K. A., HENRY B., MCDONALD G. 1994. Human ehrliehiosis in adluts after tick exposure. Ann. Inter. Med. 120(9): 730-735.
• FISHBEIN D. B., DAWSON J. E., ROBINSON L. E. 1994. Human ehrlichiosis in the United States, 1985 to 1990. Ann. Inter. Med. 120(9): 736-743.
• GUV E. C., STANEK G. 1991. Detection of Borellia burgdorferi in patient with Lyme disease by the polymerase chain reaction. J. Clin. Pathol. 44: 610-611.
• LEVIN M. L., des VIGNES F., FISH D. 1999. Disperity in the natural cycles of Borrelia burgdorferi and the agent of human granulocytic ehrlichiosis. Emerg. Infect. Dis. 5 (2): 204-208.
• MAEDA K., MARKOWITZ N., HAWLEY R. C., RISTIC M., COX D., MCDADE J. E. 1987. Human infection with Ehrlichia canis, a leukocytic Rickettsia. N. Engl. J. Med. 316(14): 853-856.
• MCDADE J. 1990. Ehrlichiosis - a disease of animals and humans. J. Infect. Dis. 161: 609-617.
• MORAIS J. D., DAWSON J. E., GREENE C., FILIPE A. R., GALHARDAS L. C., BACELLAR F. 1991. First European case of ehrlichiosis. Lancet. 338.Sept.7: 633-634.
• NUTI M., SERAFINI D. A., BASSETH D., GHIONNI A., RUSSINO F., ROMOLR P., ACRI G., LILLINI E. 1998. Ehrlichia infection in Italy. Emerg. Infect. Dis. 4(4): 663-665.
• PAROLA P., BEATI L., CAMBON M., BROUQUI P., RAOULT D. 1998. Ehrlichial DNA amplified from Ixodes ricinus (Acari: Ixodidae) in France. J. Med. Entomol. 35 (2): 180-183.
• PETROVEC M., FURLAN S. L., ZUPANC T. A., STRLE F., BROUQUI P. H., ROUX V., DUMLER J. S. 1997. Human disease in Europe caused by a granulocytic Ehrlichia species. J. Clin. Microbiol. 35 (6): 1556-1559.
• PIERARD D., LEVTCHENKO E., DAWSON J. E., LAUWERS S. 1995. Ehrlichiosis in Belgium. Lancet. 346. Nov.4: 1233-1234.
• POPOV V. L., CHEN C. M., FENG H. M., WALKER D. H. 1995. Ultrastruktural variation of cultured Ehrlichia chaffeensis. J. Med. Microbiol. 43: 411-421.
• POPOV V .. L., HAN V. C., CHEN C. M., DUMLER J. S., FENG H. M., ANDREADIS T. G., TESH R. B., WALKER D. H. 1998. Ultrastruktural differentiation of the genogrups in the genus Ehrlichia. J. Med. Microbiol. 47: 235-251.
• RIKIHISA Y. 1991. The tribe Ehrlichieae and Ehrlichial diseases. Clin. Microbiol. Rev. 4 (3): 286-308.
• SKOTARCZAK B. 1997. Detection of Borrelia burgdorferi in ticks by amplification of flagellin gene. Australian Society for Parasitology Annual Scientific Meeting and ADM 1997, July 1997, Canberra, Australia: 59.
• SKOTARCZAK B. 2000. Wykrywanie Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus metodą łańcuchowej reakcji polimerazy (PCR)*. Wiad. Parazytol. 1: 93-99.
• SKOTARCZAK B., WODECKA B. 1998. Występowanie krętków Borrelia burgdorferi sensu lato u kleszczy Ixodes ricinus w lasach województwa szczecińskiego. Wiad. Parazytol. 2: 227-232.
• SKOTARCZAK B., WODECKA B., STACHÓW A. 1998. Distribution and molecular analysis of Lyme disease Borrelia burgdorferi sensu lato isolated from ticks in the recreative areas of Szczecin. 18. Tagung der Deutchen gesellschaft fur Parasitologie e. V in Dresden, 24-28.III.1998, p.122.
• SKOTARCZAK B., WODECKA B., STACHÓW A. 1998a. Polymerase chain reaction primers from flagellin gene sequences for specific detection of the agents of Lyme disease. J. Appl. Genetics. 39: 165.
• WODECKA B., SKOTARCZAK B. 2000. Genetyczna zmienność Borrelia burgdorferi s. 1. u kleszczy Ixodes ricinus zebranych w północno-zachodniej Polsce. Wiad. Parazytol., w druku.
• WEINSTEIN R. S. 1996. Human ehrlichiosis. Am. Fam. Physic. Nov. 1: 1971- 1976.