Histopathological examination of ovaries in bitches after experimental zearalenone mycotoxicosis

• Autorzy: Gajecka M. , Obremski K. , Jakimiuk E. , Skorska-Wyszynska E. , Zielonka L. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

The present study deals with the influence of experimental ZEA mycotoxicosis on histopathological lesions in ovaries of bitches, which were administered zearalenoneperos during anestrus phase for one hundred days. The experiment was performed on 9 sexually mature, clinically healthy bitches. The animals assigned into two experimental groups received zearalenone per os at two doses, 25 μg/kg b.w. and 50 μg/kg b.w., respectively: the bitches from control group received placebo per os. On the last day of zearalenone intoxication, the bitches were ovariohystorectomized. Histopathology and immunohistochemistry were performed. The study revealed that zearalenone and its metabolites caused profound regressive lesions: granular cells degeneration and atrophy. Numerous edemas and blood extravasations were also found. The intensity of these changes was significantly dose dependent. Furthermore, in ovarian cells and tissues of both experimental groups, no reaction for PCNA antigen was observed. In conclusion, zearalenone and its metabolites exerts unfavorable effects on the morphology of ovaries in bitches.

Słowa kluczowe

EN

dog; bitch; mycotoxicosis; zearalenone mycotoxicosis; histopathology; experiment; uterus; ovary; immunohistochemistry; granular cell; degeneration; atrophy; ovarian cell; ovarian tissue; antigen; morphology

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2008
• Tom: 11
• Numer: 4
• Opis fizyczny: p.363-366,fig.,ref

Twórcy

autor

Gajecka M.

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Obremski K.

autor

Jakimiuk E.

autor

Skorska-Wyszynska E.

autor

Zielonka L.

autor

Gajecki M.

Bibliografia

• Fink-Gremmels J, Malekinejad H (2007) Clinical effects and biochemical mechanisms associated with exposure to the mycoestrogen zearalenone. Anim Feed Sci Technol 137: 326-341.
• Gajęcka M, Jakimiuk E, Polak M, Otrocka-Domagala I, Janowski T, Zwierzchowski W, Obremski K, Zielonka Ł, Apoznański J, Gajęcki M (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol J Vet Sci 7: 59-66.
• Gajęcka M, Janowski T, Jakimiuk E, Polak M, Pod- halicz-Dzięgielewska M, Rotkiewicz T, Otrocka- -Domagala I, Obremski K, Zielonka Ł, Gajęcki M (2007)
• Histopathological and immunohistochemical examin­ation of the uterus in bitches following zearalenon my- cotoxicosis. Pol J Vet Sci 10: 143-151.
• Whitehead SA, Rice S (2006) Endocrine-disrupting chemi­cals as modulators of sex steroid synthesis. Best Pract Res Clin Endocrinol Metab 20: 45-61.
• Zanghf A, Catone G, Marino G, Quartuccio M, Nicntina PA (2007) Endometrial polypoid adenomyomatosis in a bitch with ovarian granulosa cell tumour and pyometra. J Comp Pathol 136: 83-86.
• Zinedine A, Soriano JM, Molto JC, Manes J (2007) Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: An oestrogenic mycotoxin. Food Chem Toxicol 45: 1-18.