Electroporated intact BY-2 tobacco culture cells as a model of transient expression study

• Autorzy: Koscianska E. , Wypijewski K.
• Języki publikacji: EN

Abstrakty

EN

Transfer of foreign genes into plant cells can be accomplished by several methods: agrobacterium-mediated, microinjection, biolistic particle bombardment and electro- poration. The last one is frequently used for transfection of plant protoplasts for tran­sient gene expression. Electroporation is a simple procedure and allows transfecting a large number of cells at one time. Square wave-modulated porators are the most effi­cient for introducing expression cassettes into plant protoplasts. Based on a protocol developed by Wu & Feng (Plant Cell Reports, 1999, 18, 381-386), we optimized condi­tions for transfection of intact Nicotiana tabacum BY-2 cells using square wave-modu­lated electroporator. To simplify screening for transfected gene expression we used constructs with a GFP marker gene.

Słowa kluczowe

EN

gene; cell; culture cell; gene expression; transient gene expression; plant protoplast; plant cell; BY-2 cell; tobacco; cytoplasm; DNA; transfection

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 3
• Opis fizyczny: p.657-661,fig.

Twórcy

autor

Koscianska E.

• Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Wypijewski K.

Bibliografia

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