Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of cultured human hepatoma HepG2 cells

• Autorzy: Koj A. , Jura J.
• Języki publikacji: EN

Abstrakty

EN

The systemic inflammatory reaction (acute phase response) is induced by many nox­ious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1/ß) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized plasma proteins (acute phase proteins) has been extensively studied. Here we describe a model system of cultured human hepatoma HepG2 cells stimulated with IL-1/ ß to evaluate the trans­criptome induced by this cytokine during 24 h of treatment. By using differential dis­play analysis we found IL-1/ß-induced upregulation of several genes coding for cellular trafficking/motor proteins, proteins participating in the translation machinery or in­volved in posttranscription/posttranslation modifications, proteases, proteins in­volved in cellular metabolism, activity modulators, proteins of the cell cycle machin­ery and also some new proteins so far functionally not classified.

Słowa kluczowe

EN

interleukin 1; cell cycle; gene; man; activity modulator; HepG2 cell; hepatoma; protein; cellular metabolism; new protein; inflammatory reaction

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: p.573-582,fig.

Twórcy

autor

Koj A.

• Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

autor

Jura J.

Bibliografia

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