Breakdown of [3H]-thymidine and the inability to measure the proliferation of lymphocytes in whole blood cultures

• Autorzy: Stec J , Reichert M. , Rachubik J.
• Języki publikacji: EN

Abstrakty

EN

Several components of sheep blood extensively catabolize nucleosides, including thymidine. Part of the radioactivity from tritiated thymidine, in whole blood cultures, is quickly rendered unincorporable as the compound is metabolized to thymine and further breakdown products. Thus, cells continue to proliferate without incorporating radioactivity from the medium. Furthemore, variability in the degree of catabolism varied from animal to animal, so that neither measurement of the depletion of radioactivity from the medium nor measurement of the amount of label incorporated into cultures can be used as a quantitative index of the cell proliferation or immunocompetence.

Słowa kluczowe

EN

blood culture; proliferation; thymidine; breakdown; lymphocyte; [3H]-thymidine; radioactivity

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1999
• Tom: 43
• Numer: 1
• Opis fizyczny: p.25-32,fig.

Twórcy

autor

Stec J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Reichert M.

autor

Rachubik J.

Bibliografia

• 1. Ahmed J.S., Rothert M., Steuber S., Schein E.: J. vet. Med. B 36, 584, 1989.
• 2. Aisenberg A.C.: Nature 205, 1233, 1965.
• 3. Bodycote J., Wolf S.: Proc. natl Acad. Sci USA 83, 4749, 1986.
• 4. Briers T.W., Desmaretz C.: Anal. Biochem. 211 , 173, 1993.
• 5. Burrells C., Wells P.W.: Res. vet. Sci. 23, 84, 1977.
• 6. Carr M.C., Stites D.P., Fudenberg H.H.: Cell. Immunol. 5, 21, 1972.
• 7. De Jong B., Anders G.J.P.A., Van der Meer I.H., Zijlstra J., lndenburg V.J.S.: J. immunol. Methods 50, 11, 1982.
• 8. Desgranges C., Razaka G., Lamazire J.M.D., Rabaud M., Bricaud H., Boiseau M.: C.R. Seances Acad. Sci. D 291, 405, 1980.
• 9. Doldi K, Leroux M., Augustin R., Kirchner H., Kalden J.R: J. Interferon Res. 5, 55, 1985.
• 10. Galo R., Perry S.: J. clin. Invest. 48, 105, 1969.
• 11. Hallgren H.M., Buckley C.E., Gilbertsen V.A., Yunis E.J.: J. Immunol. 111, 1101. 1973.
• 12. Heldin C.H., Wasteson A., Westemark B.: Exp. Cell Res. 109, 429, 1977.
• 13. Hein W.R., Shelton J.N., Simpson-Morgan M.W., Morris B.: Immunology 64, 621, 1988.
• 14. Inokuma H., Yoshida T., Onishi T.: J. vet. med. Sci. 57, 971, 1995.
• 15. Jensen P.T., Christensen K.: Vet. Immunol. Immunopath. 2, 121, 1981.
• 16. Kay J.E., Oppenheim J. J., Mergenhagen S.E.: Proc. Soc. exp. Biol. Med. 138, 112, 1971.
• 17. Kelley K.W., Osborne C.A., Evermann J.F., Parish S.M., Hinrichs D.J.: Can. J. comp. Med. 45, 249, 1981.
• 18. Kelley D.W., Osborne C.A., Evermann J.F., Parish S.M., Gaskins C.T.: J. Dairy Sci. 65, 1514, 1982.
• 19. Kostro K., Wiktorowicz K.: Acta vet. hun. 40, 39, 1992.
• 20. Kumar R.K.: J. immunol. Methods 25, 239, 1979.
• 21. Larsen H.J.: Res. vet. Sci. 27, 334, 1977.
• 22. Lijnen P., Saavefra A., Petrov V.: Clin. chim. Acta 264, 91, 1997.
• 23. Menne S., Maschke J., Tolle T., Kreuzfelder E. Grosse-Wilde H., Roggendorf M.: Arch. Virol. 142, 511, 1997.
• 24. Morimoto K., Wolf S.: Nature 288, 604, 1980.
• 25. Olivieri G., Bodycote J., Wolf S.: Science 223, 594, 1984.
• 26. Oppenheim J.J., Whang J., Frei E.: Blood 26, 121, 1965.
• 27. Pauly J.L., Schuller M.G., Zelcer A.A., Germain M.J.: Experientia 33, 668, 1977.
• 28. Pauly J.L., Schuller M.G., Zelcer A.A., Kirss T.A., Gore S.S., Germain M.J.: J. natl Cancer Inst. 58, 1587, 1977.
• 29. Pero R.W., Johnson D., Olson A.: Cancer Res. 44, 4955, 1984.
• 30. Russel P.H.: Res. vet. Sci. 28, 39, 1980.
• 31. Stec J., Reichert M.: Folia vet. 41, 17, 1997.
• 32. Taylor J.K., Woods P.S., Hughes W.L.: Proc. natl Acad. Sci. USA 43, 122, 1957.
• 33. Tierney T.J., Simpson-Morgan M.W.: Vet. Immunol. Immunopath. 59, 49, 1997.
• 34. Tierney T.J., Simpson-Morgan M.W.: Vet. Immunol. Immunopath. 57, 229, 1997.
• 35. Young J.L., Daser A., Beverley P.C.P.: J. immunol. Methods 182, 177, 1995.
• 36. Weksler M.E., Hutteroth T.H.: J. clin. Invest. 53, 99, 1974.
• 37. Whitbread T.J., Rowan T.G.: Res. vet. Sci. 40, 161, 1986