Effectiveness of chemical agents in removing platinum from DNA isolated from cisplatin-treated HL-60 cells

• Autorzy: Olinski R. , Briggs R.C. , Basinger M. , Jones M.M.
• Języki publikacji: EN

Abstrakty

EN

Human promyelocytic leukemia cells,. HL-60, were treated with cisplattn [cis-diamminedichloroplatinum (II)] (2 itiM, 1 h). DNA- eisplatin-protein complexes were isolated and exposed to thiourea (1M), NaCN (100 mM), diethyldithio-carbamate (500 mM), or tf-methyl-D- glucamine dithiocarbamate (500 mM) for 12 h. The release of platinum was measured by atomic absorption spectroscopy. Sodium cyanide was the most effective agent, releasing about 90% of the DNA-bound platinum. Thiourea was the least effective agent, while dithiocar- bamates exhibited an intermediate. The ability of the same group of agents to split the proteins off from the protein-cisplatin-DNA complex was also evaluated and similarly dithiocarbamate were also the most effective.

Słowa kluczowe

EN

chemical agent; cell; platinum; DNA

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1992
• Tom: 39
• Numer: 4
• Opis fizyczny: p.327-334,fig.

Twórcy

autor

Olinski R.

• Medical School, M.Curie-Sklodowskiej 9, 85-094 Bydgoszcz, Poland

autor

Briggs R.C.

autor

Basinger M.

autor

Jones M.M.

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