A simple method for visualization of phenolics exudation by roots of intact lupin plants; the effect of nitrate and pH

• Autorzy: Wojtaszek P. , Peretiatkowicz M.
• Języki publikacji: EN

Abstrakty

EN

Phenolics exudation by imbibed seeds and roots of intact lupin plants (Lupinus albus L.) was studied during the first 4 days of growth by a new agar test with specific reagents for phenolics (Gibbs reagent, Naturstoffreagenz A). Comparative studies of the phenolics exudation reveal that legumes exude different phenolics (even if not qualitatively, then at least quan­titatively) than oat. The exudation of phenolics starts very quickly after the imbibition of seeds and can be visualized as early as 24 h after sowing. In older seedlings, the exudation of phenolics can be detected along root zones and is influenced by nitrate and pH. At acidic pH, nitrate reduces phenolics exudation, but at pH 7.5 the exudation of phenolics becomes restricted to only some root zones. Nitrate must be present in the rooting media for at least 24 h to cause visible changes in the pattern of exudation at different pH values.

Słowa kluczowe

EN

phenol; nitrate effect; lupin; root; exudation; pH effect

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1992
• Tom: 39
• Numer: 4
• Opis fizyczny: p.307-316,fig.

Twórcy

autor

Wojtaszek P.

• Institute of Bioorganic Chemistry, Noskowskiego 12, 61-704 Poznan, Poland

autor

Peretiatkowicz M.

Bibliografia

• 1. Rovira, A. D. (1969) Plant root exudates. Bot. Rev., 35, 35 - 57.
• 2. Swain, T, (1977) Secondary compounds as protective agents. Annu. Rev: Plant Physiol, 28,479 - 501.
• 3. Rice, E. L. (1984) Allelopathy. 2nd edition, pp. 422. Academic Press, Inc., London.
• 4. Ingham, J. L. (1972) Phytoalexins and other natural products as factors in plant disease resistance. Dot. Rev., 38,343 - 424.
• 5. Lynn, D. G. & Chang, M. (1990) Phenolic signals in cohabitation: Implications for plant development. Annu. Rev: Plant Physiol. Plant Mol. Biol, 41,497 - 526.
• 6. Long, S. R. (1989) Rhizobium - legume nodulation: Life together in the underground. Cell 56,203 - 214.
• 7. Rolfe, B. G. (1988) Flavones and isoflavones as inducing substances of legume nodulation. BioFactors, 1,3 -10.
• 8. Redmond, J. W., Batley, M., Djordjevic, M. A., Innes, R. W., Kuempel, P. L. & Rolfe, B. G. (1986) Flavones induce expression of nodulation genes in Rhizobium. Nature (London), 323,632 - 635.
• 9. Peters, N. K. & Long, S. R. (1988) Alfalfa root exudates and compounds which promote or inhibit induction of Rhizobium meliloti nodulation genes. Plant Physiol, 88,396 - 400.
• 10. Streeter, J. (1988) Inhibition of legume nodule formation and N2 fixation by nitrate. CRC Crit Rev. Plant Sci, 7,1 - 23.
• 11. d'Arcy-Lameta, A. (1986) Study of soybean and lentil root exudates. 0. Identification of some polyphenolic compounds, relation with plantlet physiology. Plant Soil, 92,113 - 123.12. Elkan, G. H. (1984) Taxonomy and metabolism of Rhizobium and its genetic relationships; in Biological nitrogen fixation. Ecology, technology and physiology (Alexander, M., ed.), pp. 1 - 38. Plenum Press, New York, London.
• 13. King, F. E., King, T. J. & Manning, L. C. (1957) An investigation of the Gibbs reaction and its bearing on the constitution of jacareubin. /. Chem. Soc.y 563 - 566.
• 14. Stahl, E. (ed.) (1969) Thin-Layer Chromatography. A Laboratory Handbook. 2nd edition, pp. 1041. Springer-Verlag, Berlin, Heidelberg, New York.
• 15. Stobiecki, M. & Wojtaszek, P. (1990) Application of gas chromatography - mass spectrometry to the identification of isoflavonoids in lupine root extracts. J. Chromatogr, 508, 391 - 398.
• 16. Weisenseel,M. H.,Dom, A. & Jaffe, L. F. (1979) Natural H+ currents traverse growing roots and root hairs of barley (Hordeitm vulgare L.). Plant Physiol.164, 512 - 518.
• 17. Marschner, H., Romheld, V. & Ossenberg-Neuhaus, H, (1982) Rapid method for measuring changes in pH and reducing processes along roots of intact plants. Z Pflanzen- physiol, 105,407 - 416.
• 18. Marschner, H. & Romheld, V. (1983) In vivo measurement of root-induced pH changes at the soil-root interface: Effect of plant species and nitrogen source. Z Pflanzen- physiol, Ill, 241-251.
• 19. Stevens, D. L. & Oaks, A. (1973) The influence of nitrate on the induction of nitrate reductase in maize roots. Can. J. Bot., 51,1255 - 1258.
• 20. Pate, J. S., Layzell, D. B. & Atkins, C. A. (1979) Economy of carbon and nitrogen in a nodulated and nonnodulated (N03-grown) legume. Plant Physiol., 64, 1083 -1088.
• 21. Raven, J. A. & Smith, F. A. (1976) Nitrogen assimilation and transport in vascular land plants in relation to intracellular pH regulation. New PhytoL, 76,415 - 431.
• 22. Hattori, T. & Ohta, Y. (1985) Induction of phenylalanine ammonia-lyase activation and isoflavone glucoside accumulation in suspension-cultured cells of red bean, Vigna angularis, by phytoalexin elicitors, vanadate, and elevation of medium pH. Plant Cell Physiol, 26,1101 -1110.
• 23. Kneusel, R. E., Matern, U. & Nicolay, K. (1989) Formation of irans-caffeoyl-CoA from trans-A-coumaroyl-CoA by Zn -dependent enzymes in cultured plant cells and its activation by an elicitor-induced pH shift. Arch. Biochem. Biophys., 269,455 - 462,