Molecular epidemiology of antibiotic-resistant Escherichia coli isolated from hospitalized patients with urinary tract infections in Northern Palestine

• Autorzy: Adwan K , Abu-Hasan N. , Adwan G. , Jarrar N. , Abu-Shanab B. , Al-Masri M.
• Języki publikacji: EN

Abstrakty

EN

Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine whether this was due to intra-hospital transmission of resistant strains, clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) and all were clonally distinct. Thus, these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolates.

Słowa kluczowe

EN

human disease; patient; antibiotic resistance; DNA fingerprinting; polymerase chain reaction; urinary tract infection; Palestine

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 1
• Opis fizyczny: p.23-26,fig.,ref.

Twórcy

autor

Adwan K

• An-Najah N.University, P.O.Box 7-Nablus, Palestine

autor

Abu-Hasan N.

autor

Adwan G.

autor

Jarrar N.

autor

Abu-Shanab B.

autor

Al-Masri M.

Bibliografia

• Allen U.D., N. MacDonald, L. Fuite, F. Chan and D. Stephens. 1999. Risk factors for resistance to "first-line" antimicrobials among urinary tract isolates of Escherichia coli in children. Can. Med. Assoc. J. 160: 1436-1440.
• Bauer A.W., W.M.M. Kirby, J.C. Sherris and M. Truck. 1966. Antibiotic susceptibility testing by a standardized single disk method. J. Gen. Microbiol. 45: 493-496.
• DallaCosta L.M., K. Irino, J. Rodrigues, I.N.G. Rivera and L.RTrabulsi. 1998. Characterization of diarrhoeagenic Escherichia coli clones by ribotyping and ERIC-PCR. J. Med. Microbiol. 47: 227-234.
• Ena J., M.M. Lopez-Perezagua, C. Martinez-Peinado, M.A. Cia-Barrio and I. Ruiz-Lopez. 1998. Emergence of ciprofloxacin resistance in Escherichia coli isolates after widespread use of fluoroquinolones. Diagn. Microbiol. Infect. Dis. 30: 103-107.
• Garau J., M. Xercavins, M. Rodriguez-Carballeira, J.R. Gomez-Vera, I. Coll, D. Vidal, T. Llovet and A. Ruiz-Bremon. 1999. Emergence and dissemination of quinolone-resistant Escherichia coli in the community. Antimicrob. Agents. Chemother. 43: 2736-2741.
• Gruneberg R.N. 1994. Changes in urinary pathogens and their antibiotic sensitivities, 1971-1992. J. Antimicrob. Chemother. 33 (Suppl. A): 1-8.
• Gupta K., T.M. Hooton, C.L. Wobbe and W.E. Stamm. 1999. The prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in young women. Int. J. Antimicrob. Agents. 11: 305-308.
• Gupta K., D. Scholes and W.E. Stamm. 1999. Increasing prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in women. J.A.M.A. 281: 736-738.
• Guyot A., S.P. Barrett, E.J. Threlfalt, T. Hampton and T. Cheasty. 1999. Molecular epidemiology of multi-resistant Escherichia coli. J. Hosp. Infect. 43: 39-48.
• Hsueh P.R., M.L. Chen, C.C. Sun, W.H. Chen, H.J. Pan, L.S. Yang, S.C. Chang, S.W. Ho, C.Y Lee, W.C. Hsieh and K.T. Luh. 2002. Antimicrobial drug resistance in pathogens causing nosocomial infections at a university hospital in Taiwan, 1981-1999. Emerg. Infect. Dis. 8: 63-68.
• Lepelletier D., N. Caroff, A. Reynaud and H. Richet. 1999. Escherichia coli: epidemiology and analysis of risk factors for infections caused by resistant strains. Clin. Infect. Dis. 29: 548-552.
• Livermore DM. 1995. β-lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 8: 557-584.
• National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disc susceptibility tests: tentative standards, vol. 3, No. 24, NCCLS document M2-A5. Villanova, Pa National Committee for Clinical Laboratory Standards, 1993.
• Okeke I.N., S.T. Fayinka and A. Lamikanra. 2000. Antibiotic resistance in Escherichia coli from Nigerian students, 1986-1998. Emerg. Infect. Dis. 6: 393-396.
• Perrin M., P.Y Donnio, C. Heurtin-Lecorre, M.F. Travert and J-L. Avril. 1999. Comparative antimicrobial resistance and genomic of Escherichia coli isolated from urinary tract infections in the community and in hospitals. J. Hosp. Infect. 41: 273-279.
• Sotto A., C.M.D. Boever, P. Fabbro-Peray, A. Gouby, D. Sirot and J. Jourdani. 2001. Risk factors for antibiotic-resistant Escherichia coli isolated from hospitalized patients with urinary tract infections: a prospective study. J. Clin. Microbiol. 39: 438-444.