Usefulness of ELISA components preserved under different conditions for the detection of Hypoderma bovis antibodies

• Autorzy: Cencek T , Ziomko I. , Karamon J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the investigations was to determine the stability of ELISA components preserved under different conditions. The following components for ELISA were used: the secretory-excretory (s-e) antigen prepared from H. bovis L1 larvae, its fraction containing hypodermin A, conjugate, positive and negative control sera. Freezing at -20°C, lyophilization and preservation at 4°C were applied. Seven ELISA kits containing components preserved by the use of the described methods were prepared. The identical packets of sera were examined by ELISA directly after preparation of the kits (day „0”) and next at 3-4-week intervals for 9 months. The experiment showed that the best method for conserving ELISA components during the detection of H. bovis antibodies was lyophilization. The kits prepared in this way did not lose their characteristics for 9 months and their specificity and sensitivity were nearly 100%.

Słowa kluczowe

EN

antigen; bovine hypodermosis; diagnosis; Hypoderma bovis; ELISA test; parasitology; larva; antibody

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 2
• Opis fizyczny: p.197-204,fig.

Twórcy

autor

Cencek T

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Ziomko I.

autor

Karamon J.

Bibliografia

• 1. Boulard C.: Avantages de l'immunodiagnostic de l'hypodermose bovine etabli par hemagglutination passive et par ELISA, a partir du serum et lactoserum, sur la numeration des varons. Ann. Rech. Vet., 1985, 16, 335-43.
• 2. Cencek T., Ziomko I.: Optimum terms for collecting blood samples for the laboratory diagnosis of hypodermosis in different regions of Poland. Bull. Vet. Inst. Pulawy. (in print in this issue of the Bulletin)
• 3. Lonneaux J.F., Losson B., Pouplard L., Hernandez S., Gasca A., Martinez J., Pithan K.: Serological survey of the incidence of Hypoderma in the Province of Liege (Belgium). Improvements in Control Methods for Warble Fly in Cattle and Goats: Workshops Held in Cordoba 8 to 10 May 1991. 1992; 67-72.
• 4. Losson B., Lonneux J.F.: Hypodermose bovine: Evaluation preliminaire de la technique ELISA en vue du depistage precoce de l'affection. Ann. Med. Vet., 1991, 135, 373-375.
• 5. Martinez Moreno F.J., Wassall D.A., Becerra Martell C., Hernandez Rodriguez S.: Comparison of the use of secretory and somatic antigens in an ELISA for the serodiagnosis of hypodermosis. Vet. Parasitol., 1994, 52, 321-329.
• 6. Prieto M., Espi A., Garcia Paloma J. A., Parra F.: Effect of ivermectin treatment of anti-hypodermin C titres of Asturiana cattle naturally infected with Hypoderma lineatum. Vet. Parasitol., 1990, 35, 211-8.
• 7. Sinclair I. J., Wassall D. A.: Enzyme-linked immunosorbent assay for the detection of antibodies to Hypoderma bovis in cattle. Res. Vet., 1983, 34, 251-252.
• 8. Wassall D.A., Martinez Moreno J., Hernandez Rodrigues S., Gasca A., Pithan K.: Preliminary observations on the use of a secretory antigen in an ELISA for the serodiagnosis of hypodermosis. Improvements in Control Methods for Warble Fly in Cattle and Goats: Workshops Held in Cordoba 8 to 10 May 1991. 1992, 133-140.
• 9. Webster K.A., Giles M., Dawson C.: A competitive ELISA for the serodiagnosis of hypodermosis. Vet. Parasitol., 1997, 68, 155-164.
• 10. Ziomko I., Cencek T.: Effect of preservation conditions of Hypoderma bovis antigens on their activity in the ELISA. Bull. Vet. Inst. Pulawy, 1997, 41, 25-28.
• 11. Ziomko I., Cencek T.: The ELISA kit for the detection of Hypoderma bovis antibodies in cattle. Bull. Vet. Inst. Pulawy, 2001, 45, 205-210.