Involvement of nitric oxide in regulation of the medullary respiratory rhythm in neonatal rats

• Autorzy: Volgin D , Volgina A. , Seredenko M.
• Języki publikacji: EN

Abstrakty

EN

The NADPH-diaphorase (as a neuronal NO-synthase) reactivity in the medullary structures of the respiratory rhythm (RR) generator and the role of NO in the regulation of respiratory activity in the phrenic nerve of artificially superfused semi-isolated medulla-spinal cord preparations were investigated in newborn rats. NADPH-diaphorase positive neurons were found in all nuclei of both dorsal and ventral respiratory groups of neurons. The maximal density of stained cells was present within the rostral part of the ventrolateral medulla (VLM), in the region of the lateral paragigantocellular reticular nucleus. It was found that endogenous NO mediates the mechanism of tonic inhibitory control of the RR frequency located in the rostral VLM under normal and hypoxic conditions, and appears to be involved in generation of the basic RR by the more caudal structures of VLM. It was shown that NO biosynthesis mediates the effect of NMDA receptors activation on the RR.

Słowa kluczowe

EN

glutamate receptor; phrenic nerve; nitric oxide; drug; hypoxia; ventrolateral medulla; nicotinamide adenine dinucleotide phosphate-diaphorase; N-methyl-D-aspartate; histochemistry; neurotransmission; respiratory rhythm; electrophysiology

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2000
• Tom: 60
• Numer: 2
• Opis fizyczny: p.175-186,fig.

Twórcy

autor

Volgin D

• Ukrainian National Academy of Sciences, 4 Bogomolets St., 01024 Kiev, Ukraine

autor

Volgina A.

autor

Seredenko M.

Bibliografia

• Andrezik J.A., Chan-Palay V., Palay S.L. (1981) The nucleus paragigantocellularis lateralis in the rat. Demonstration af­ferents by the retrograde transport of horseradish peroxi­dase. Anat. Embryol. 161: 373-390.
• Budzinska K., von Euler C., Kao F.F., Pantaleo I., Yamamoto Y. (1985) Effects of graded focal cold block in rostral areas of the medulla. Acta Physiol. Scand. 124: 329-340.
• Dawson T.M., Snyder S.H. (1994) Gases as biological mess­engers: nitric oxide and carbon monoxide in the brain. J. Neurosci. 14: 5147-5159. Dillon G.H., Welsh D.E., Waldrop T.G. (1991) Modulation of respiratory reflexes by an excitatory amino acid mech­anism in the ventrolateral medulla. Respir. Physiol. 85: 55-72.
• Dun N.J., Dun S.L., Forsterman U. (1994) Nitric oxide syn­thase immunoreactivity in rat pontine medullary neurones. Neuroscience 59: 429-445.
• Euler C., von (1986) Brain stem mechanisms for generation and control of breathing pattern. In: Handbook of physio­logy. The respiratory system. Control of breathing (Eds. H.Rahn and W.O.Fenn). Vol. 2. Am. Physiol. Soc., Wash­ington, D.C. p. 1-67.
• Funk G.D., Smith J.C., Feldman J.L. (1993) Generation and transmission of respiratory oscillations in medullary slices: role of excitatory amino acids. J. Neurophysiol. 70: 1497­1515.
• Garthwaite J. (1991) Glutamate, nitric oxide and cell-cell sig­nalling in the nervous system. Trends Neurosci. 14: 60-67.
• Garthwaite J.,Boulton C.L. (1995) Nitric oxide signalling in the central nervous system. Annu. Rev. Physiol. 57: 683­706.
• Garthwaite J., Garthwaite G., Palmer R.M.J., Moncada S. (1989) NMDA receptor activation induced nitric oxide synthesis from arginine in the rat brain slices. Eur. J. Phar­macol. 172: 413-416.
• Hope B.T., Michael G.J., Knigge K.M., Vincent S.R. (1991) Neuronal NADPH-diaphorase is a nitric oxide synthase. Proc. Natl. Acad. Sci. USA. 88: 2811-2814.
• Johnston M.V. (1997) Hypoxic and ischemic disorders of in­fants and children. Brain Dev. 19: 235-239.
• Kalish B.E., Jhamandas K., Beninger R.J., Boegman R.J. (1999) Modulation of quinolitic acid-induced depletion of striatal NADPH diaphorase and enkephalinergic neurons by inhibition of nitric oxide synthase. Brain Res. 817: 151­162.
• Kline D.D., Yang T„ Huang P.L., Prabhakar N.R. (1998) Al­tered respiratory responses to hypoxia in mutant mice defi­cient in neuronal nitric oxide synthase. J. Physiol. 511: 273-288.
• Ling L., Karius D.R., Fiseus R.R., Speck D.F. (1992) En­dogenous nitric oxide required for an integrative respir­atory function in the cat brain. J. Neurophysiol. 68: 1910-1912.
• Marchenko V.A., Fenik V.B., Preobrazhenski N.N., Seredenko M.M. (1995) Respiratory activity generated by semi-iso­lated medullo-spinal preparation and recorded from the phrenic nerve of newborn rats. Neurophysiology 27: 385­395.
• Moibenko A.A., Sagach V.F., Shapoval L.N. et al. (1997) Role of endothelium and endothelium-produced biologi­cally active substances in regulation of circulation and car­diac function (in Ukrainian). Fiziol. Zh. 43: 3-18.
• Ogawa H., Mizusawa A., Kikychi Y. et al. (1995) Nitric oxide as a retrograde messenger in the nucleus tractus solitarii of rats during hypoxia. J. Physiol. 486: 495-504.
• Onimary H., Arata A., Homma J. (1987) Localization of res­piratory rhythm-generating neurones in the medulla of brainstem-spinal cord preparations from newborn rats. Neurosci. Lett. 78: 152-155.
• Petrovicky P., Nemcova V. (1995) Topographical organiza­tion of NADPH-diaphorase positive neurons and fibres in dorsal ponto-mesencephalic tegmentum in the rat brain. J. Brain Res. 36: 539-545.
• Prabhakar N.R., Cherniack N.S., Haxhiu M.A. (1995) Inhibi­tory and excitatory effects of nitric oxide on respiratory re­sponses to hypoxia. In: Ventral brainstem mechanisms and control of respiration and blood pressure (Eds. C.O.Trouth, R.M.Millis, H.F.Kiwull-Schone and M.E.Schlafke). Mar­cel - Dekker. Inc., New York, p. 393-404.
• Price R.H., Mayer B., Beitz A.J. (1993) Nitric oxide synthase neurons in rat brain express more NMDA receptor mRNA than non-NOS neurons. NeuroReport. 4: 807-810.
• Prickaert J., De Vente J., Markerink-van Ittersum M., Stein­busch H.W. (1998) Behavioural, neurochemical and neuroanatomical effects of chronic postnatal N-nitro-L-ar- ginine methyl ether treatment in neonatal and adult rats. Neuroscience 87: 181-195.
• Roche A.K., Cook M„ Wilcox G.L., Kajander K.C. (1996) A nitric oxide synthesis inhibitor (L-NAME) reduces licking behavior and Fos-labeling in the spinal cord of rats during formalin-induced inflammation. Pain 66: 331-341.
• Suzue T. (1984) Respiratory rhythm generation in the in vitro brain stem-spinal cord preparation of the neonatal rat. J. Physiol. 354: 173-184.
• Trzebski A., Sato Y„ Suzuki A., Sato A. (1995) Inhibition of nitric oxide synthesis potentiates the responsiveness of ca­rotid chemoreceptors to systemic hypoxia in the rat. Neu- rosci. Lett. 190: 29-32.
• Vincent S.R., Kimura H. (1992) Histochemical mapping of ni­tric oxide synthase in the rat brain. Neuroscience 46: 755­784.
• Volgin D.V., Marchenko V.A., Seredenko M.M., Vasilenko D.A. (1998) Involvement of NMDA receptors in the con­trol of respiratory rhythm generated by medullo-spinal preparations of early postnatal rats. Neurophysiology 30: 101-106.