Evidence for plant ribosomal 5S RNA involvement in elongation of polypeptide chain biosynthesis

• Autorzy: Shaikhin S. , Sobkiewicz A. , Barciszewski J. , Twardowski T.
• Języki publikacji: EN

Abstrakty

EN

A series of short oligo-DNA probes (8-10-mers) complementary to various regions of the plant ribosomal 5S ribonucleic acid (5S rRNA) have been synthesized. The results of their hybridization to free 5S rRNA and to ribosomes pointed to the availability of nucleotides in loop "C" for complexation. We found a correlation between hybridization of selected oligonucleotides and their inhibitory effect on enzymatic binding of Phe-tRNA and poly(Phe) synthesis on wheat germ 80S ribosomes. Evidence was obtained for involvement of 5S rRNA in the elongation of polypeptide chain during protein biosynthesis. 5S rRNA seems to play a critical role in protein biosynthesis, probably through causing conformational changes of loop C.

Słowa kluczowe

EN

RNA; ribosome; elongation factor; polypeptide chain; wheat germ; biosynthesis; translation; DNA; antisense DNA probe; plant

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1994
• Tom: 41
• Numer: 1
• Opis fizyczny: p.57-62,fig.

Twórcy

autor

Shaikhin S.

• Institute of Bioorganic Chemistry, ul.Z.Noskowskiego 12, 61-704 Poznan, Poland

autor

Sobkiewicz A.

autor

Barciszewski J.

autor

Twardowski T.

Bibliografia

• 1. Erdmann, V.A. (1976) Structure and function of 5S and 5.8S RNA Pwgr. Nucl. Acid Res. Mol. Biol. 18,45-90.
• 2. Nomura, M. & Erdmann, V.A. (1970) Recon­stitution of 50S ribosomal subunits from dissociated molecular components. Nature (London) 228,744-748.
• 3. Hartmann, R.K., Vogel, D.M., Walker, R.T. & Erdmann, V.A. (1988) In vitro incorporation of eubacterial, archaebacterial and eukaryotic 5S rRNAs into large ribosomal subunits of Bacillus stearothmnophilus. Nucl. Acids Res. 16, 3511-3524.
• 4. Pace, B., Matthews, E.A., Johnson, K.D., Cantor, C.R. & Pace, N.R. (1982) Conserved 5S rRNA complement to tRNA is not required for protein synthesis. Proc. Natl. Acad. Sci. U.S.A. 79,36^0.
• 5. Lorenz, S., Hartmann, R.K., Piel, N., Ulbrich, N. & Erdmann, V.A. (1987) Structural analysis of 5S rRNA, 5S rRNA-protein complexes and ribo­somes employing RNase H and d(GTTCGG). Eur. J. Biochem. 163,239-246.
• 6. Barciszewska, M.Z., Lorenz, S., Erdmann, V.A. & Barciszewski, J. (1990) Structural analysis of plant ribosomal 5S RNAs. Visualisation of novel tertiary interactions by cleavage of lupin and wheat 5S rRNAs with ribonuclease H. Biochim. Biophys. Acta 1087,68-72.
• 7. Joachimiak, A., Nalaskowska, M., Barciszew­ska, M., Barciszewski, J. & Mashkova, T. (1990) Higher plant 5S rRNAs share common secon­dary and tertiary structure. A new three domains model. Int. J. Biol. Macromol. 12, 321-327.
• 8. Barciszewska, M.Z., Huang, H.-W., Marshall, A.G., Erdmann, V.A. & Barciszewski, J. (1992) Biochemical and NMR spectroscopy evidence for a new tertiary A-U base pair in lupin ribosomal 5S RNA structure. J. Biol. Chem. 267, 16691-16695.
• 9. Twardowski, T., Pulikowska, J. & Wiewi6- rowski, M. (1981) Inhibitory effect of selected quinolizidine alkaloids and their derivatives and analogues on the Phe-tRNA binding to ribosomes. Bull. Pol. Ac.: Biol. 29,129-140.
• 10. Pulikowska, J. & Twardowski, T. (1982) The elongation factor 1 from wheat germ: structural and functional properties. Acta Biochim. Polon. 29,245-258.
• 11. Grzywacz-Bohun, E. & Twardowski, T. (1992) Blocking of the function of a-sarcin domain of 28S ribosomal RNA using the synthetic oligonucleotides as antisense DNA probes. Acta Biochim. Polott. 39,65-73.
• 12. Lorenz, S., Hartmann, R.K., Schultze, S., Ulbrich, N. & Erdmann, V.A. (1989) Structural analysis of procaryotic and eucaryotic 5S rRNAs by RNase H. Biochimie 71,1185-1191.
• 13. Nygard, O. & Nilsson, L. (1987) The ribosomal binding site for eukaryotic elongation factor EF-2 contains 5S ribosomal RNA. Biochim. Biophys. Acta 908,46-53