ELISA kit for detection of Hypoderma bovis antibodies in cattle

• Autorzy: Ziomko I , Cencek T.
• Języki publikacji: EN

Abstrakty

EN

The aim of these investigations was to prepare the antigen and control sera for the ELISA. Positive control sera were obtained from 163 cows showing clinical symptoms of H. bovis infection and negative control sera came from 60 cows free from the infection. The antigen for the ELISA was prepared from H. bovis L1 larvae. Three basic fractions were isolated from whole excretory/secretory antigen by elution from gel and by preparative isoelectrofocusing. Using the Western-Blotting method it was demonstrated that Hy A (22-24 kDa and pI 4-5) had the best antigenic property. As can be seen from these investigations, the developed ELISA antigen for the detection of H. bovis antibodies in cows is highly specific and sensitive.

Słowa kluczowe

EN

diagnosis; Poland; cattle; Hypoderma bovis; ELISA test; parasitology; antibody

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 2
• Opis fizyczny: p.205-210,fig.

Twórcy

autor

Ziomko I

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Cencek T.

Bibliografia

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