Opracowanie biotynylowanej sondy genetycznej wirusa choroby Aujeszkyego

• Autorzy: Lipowski A , Kochan G. , Pejsak Z. , Szewczyk B.
• Języki publikacji: PL

Abstrakty

EN

A simple and quick method of viral DNA purification from RK 13 infected with NIA-3 strain of ADV cell culture yielded DNA which was easily digested with its enzyme restriction. Moreover, it was of a sufficient purity for a restriction pattern (RFP) analysis. The purified DNA was labelled with photobiotin to obtain a biotinylated genetic probe. The full length DNA probe that was developed was found to be very sensitive and specific when it was used for hybridization with ADV DNA. Furthermore, the forenamed biotinylated probe greatly enhanced the detection limits of restriction fragments compared to RFP analysis in ethidium bromide stained agarose gel. These results indicate that the described probe can be used for analysis and characterization of ADV genomes even if only minute amounts of viral DNA are available.

Słowa kluczowe

PL

czynniki chorobotworcze; wirus choroby Aujeszkyego; sonda znakowana biotyna; sondy genetyczne; wykrywanie wirusow

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1994
• Tom: 50
• Numer: 01
• Opis fizyczny: s.14-17,bibliogr.

Twórcy

autor

Lipowski A

• Instytut Weterynarii, Al.Partyzantow 57, 24-100 Pulawy

autor

Kochan G.

autor

Pejsak Z.

autor

Szewczyk B.

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