Effect of two different ambient ozone concentrations on antioxidative enzymes in leaves of two tobacco cultivars with contrasting ozone sensitivity

• Autorzy: Bandurska H , Borowiak K. , Miara M.
• Języki publikacji: EN

Abstrakty

EN

Eight-week-old tobacco (Nicotiana tabacum L.) Bel W3 (ozone sensitive) and Bel B (ozone resistant) cultivars were exposed to ozone for two weeks at two sites with differing tropospheric ozone levels in five independent series from May 27 to July 25, 2004. After each exposition, the degree of ozone-caused visible leaf damage and the activity of APX, GuPX, and SOD were examined. Visible leaf damage was observed only in the sensitive cultivar; the resistant one did not exhibit any external symptoms. Three-way ANOVA revealed that the activity of all enzymes varied by exposure site, series and cultivar effects. Significant correlations between GuPX activity in the two cultivars and with the degree of leaf damage to the sensitive cultivar were found. This indicates that GuPX activity in the sensitive as well as in the resistant cultivars track changes in tropospheric ozone levels. The positive correlation between ozone level and APX activity in the resistant cv. Bel B, which did not reveal visible symptoms, indicates that this enzyme may contribute to detoxication of H2O2 and alleviation of oxidative damage caused by O3

Słowa kluczowe

EN

ambient ozone; ozone concentration; antioxidative enzyme; leaf; tobacco; plant cultivar; ozone sensitivity; tropospheric ozone; ascorbate peroxidase; guaiacol peroxidase; superoxide dismutase; Nicotiana tabacum

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2009
• Tom: 51
• Numer: 2
• Opis fizyczny: p.37-44,fig.,ref.

Twórcy

autor

Bandurska H

• Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Borowiak K.

autor

Miara M.

Bibliografia

• Apel K, and Hirt H. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review Plant Biology 55: 373-399.
• Ashmore MR, and Ainsworth N. 1995. The effects of ozone and cutting on the species composition of artificial grassland communities. Functional Ecology 9: 708-712.
• Bandurska H. 2002. The effect of water deficit on the activity of hydrogen peroxide-scavenging enzymes in two barley genotypes. Acta Societatis Botanicorum Poloniae 71: 307-310.
• Beauchamp CH, and Fridovich J. 1971. Superoxide dismutase: improved assays and assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-287.
• Bernardi R, Nali C, Ginestri P, Pugliesi C, Lorenzini G, and Durante M. 2004. Antioxidant enzyme isoforms on gels in two poplar clones differing in sensitivity after exposure to ozone. Biologia Plantarum 48: 41-48.
• Blum O, Didyk N. 2007. Study of ambient ozone phytotoxicity in ukraine and ozone protective effect of some antioxidants Journal of Hazardous Materials 149(3): 598-602.
• Borowiak K. 2005. The evaluation of visible leaf injury of tobacco plants caused by tropospheric ozone in the Poznań city and surrounding areas in 2002-2004. Prace Komitetu Nauk Rolniczych i Komitetu Nauk Leśnych PTPN: 57-66. (in Polish).
• Borowiak K, Zbierska J, and Jankowiak-Krysiak D. 2005. Growth and development of tobacco plants in the presence of tropospheric ozone. Prace Komitetu Nauk Rolniczych i Komitetu Nauk Leśnych PTPN 98/99: 47-55. (In Polish).
• Borowiak K, Drzewiecka K, Goliński P, and Zbierska J. 2007. Physiological reaction of tobacco plants to ambient air pollution with tropospheric ozone - preliminary studies. Electronic Journal of Polish Agricultural Universities, Environmental Development 10(1).
• Borowiak K, Rucińska-Sobkowiak R, Rymer K, Gwóźdź EA, and Zbierska J. 2009. Biochemical markers of tropospheric ozone: experimentation with test-plants. Polish Journal of Ecology 57: 3-14.
• Bradford M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
• Burkey KO, Wei C, Easond G, Ghosh P, and Fenner G. 2000. Antioxidant metabolite levels in ozone-sensitive and tolerant genotypes of snap bean. Physiologia Plantarum 110: 195-200.
• Calatayud A, Iglesias DJ, Talón M, and Barreno E. 2003. Effects of 2-month ozone exposure in spinach leaves on photosynthesis, antioxidant systems and lipid peroxidation. Plant Physiology and Biochemistry 41: 839-845.
• Castagna A, and Ranieri A. 2009. Detoxification and repair process of ozone injury: From o3 uptake to gene expression adjustment. Environmental Pollution 157: 1461-1469.
• Chernikova T, Robinson JM, Lee EH, and Mulchi CL. 2000. ozone tolerance and antioxidant enzyme activity in soybean cultivars. Photosynthesis Research 64: 15-26.
• Chevone BJ, and Linzon SN. 1988. Tree Decline in North America. Environmental Pollution 50(1-2): 87-89.
• Diara C, Castagna A, Baldan B, Mensuali Sodi A, Sahr T, Langebartels C, Sebastiani L, and Ranieri A. 2005. Differences in the kinetics and scale of signaling molecule production modulate the ozone sensitivity of hybrid poplar clones: the roles of H202, ethylene and salicylic acid. New Phytologist 168: 351-364.
• Directive 2002/3/EC of the European Parliament and of the Council of 12.02.2002 relating to ozone in the ambient air. Official Journal of the European Communities 9.3.2002. L. 67/14.
• Foyer CH-H, Lelandais M, and Kunert KJ. 1994. Photooxidative stress in plants. Plant Physiology 92: 696-717.
• Godzik B. 1997. Surface ozone concentrations in southern Poland: tobacco cultivar exposure study. Fragmenta Floristica et Geobotanica 42: 161-172.
• Godzik B. 2000. The measurements of tropospheric ozone concentration in southern Poland using the passive samplers and plant bioindicators. Archives of Environmental Protection 26(2): 7-19.
• Hammerschmidt R, Nucles EM, and Kuc J. 1982. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiological Plant Pathology 20: 73-82.
• Heggestad HE. 1991. Origin of Bel-W3, Bel-C and Bel-B in tobacco varieties and their use as indicators of ozone. Environmental Pollution 74: 264-291.
• Jeffries HE, and Tonnesen S. 1994. A comparison of two photochemical reactions mechanisms using mass balance and process analysis. Atmospheric Environment 28(18): 2991-3003.
• Klumpp A, Ansel W, Klumpp G, and Pickl C. 1999. European network for the assessment of air quality by the use of bio-indicator plants. Criteria for the selections of bioindicator stations. instructions for cultivation, exposure, injury assessment and sampling of bio-indicator species. Universitat Hohenheim, Stuttgart. Draft guide- line,79 pp.
• Klumpp A, Klumpp G, and Ansel W. 2004. Urban air quality in Europe - results of three years standardized biomonitoring studies. In: Klumpp A [eds], Urban Air Pollution, Bioindication and Environmental Awareness, 25-58. Cuvillier Verlag, Göttingen.
• Klumpp A, Ansel W, Klumpp G, Vergne P, Sifakis N, Sanz MJ, Rasmussen S, Ro-Poulsen H, Ribas A, Penuelas J, Kambezidis H, Heg S, Garrec JP, and Calatayud V. 2006. Ozone pollution and ozone biomonitoring in European cities. Part II. Ozone-induced plant injury and its relationship with descriptors of ozone pollution. Atmosphere Environment 40: 7437-7448.
• Krupa SV, Tonneijck AEG, and Manning WJ. 1998. Ozone. In: Flagler R.B. [ed.], Recognition of Air Pollution Injury to Vegetation: A Pictorial Atlas, 2.1-2.13. Air & Waste Management Association, Pittsburgh.
• Langebartels CH, Wohlgemuth H, Kschieschan S. Grün S, and Sandermann H. 2002. Oxidative burst and cell death in ozone-exposure of plants. Plant Physiology and Biochemistry 40: 567-575.
• Nakano Y, and Asada K. 1987. Purification of ascorbate peroxidase, its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiology 28: 131-140.
• Nali C, Pucciariello C, Mills G, and Lorenzini G. 2005. On the different sensitivity of white clover clones to ozone: physiological and biochemical parameters in a multivariate approach. Water Air and Soil Pollution 164: 137-153.
• Nali C, Ferretti M, Pellegrini M, and Lorenzini G. 2001. Monitoring and biomonitoring of surface ozone in Florence, italy. Environmental Monitoring and Assessment 69: 159-174.
• Ott L. 1984. An Introduction to Statistical Methods and Data Analysis. pWs publishers, Boston.
• Pasqualini S, Batini P, Ederli L, Porceddu A, Piccioni C, Marchis DE, and Antonielli M. 2001. Effects of shortterm ozone fumigation on tobacco plants: response of scavenging system and expression of the glutathione reductase. Plant, Cell and Environment 24: 245-252.
• Pell EJ, Schlagnhaufer CD, and Arteca RN. 1997. Ozone- induced oxidative stress: Mechanisms of action and reaction. Physiologia Plantarum 100: 264-273.
• Pitcher LH, and Zilinskas BA. 1996. Overexpression of cop- per/zinc superoxide dismutase in the cytosol of transgenic tobacco confers partial resistance to ozone-induced foliar necrosis. Plant Physiology 110: 583-588.
• Pukacka S, and Pukacki P. 2000. Seasonal changes in antioxidant level of Scots pine (Pinus silvestris L.) needles exposed to industrial pollution. II. Enzymatic scavengers activities. Acta Physiologiae Plantarum 22: 457-464.
• Ranieri A, Castagna A, Pacini J, Baldan B, Mensuali-Sodi A, and Soldatini GF. 2003. Early production and scavenging of hydrogen peroxide in apoplast of sunflowers plants exposed to ozone. Journal of Experimental Botany 54: 2529-2540.
• Ribas A, and Penuelas J. 2003. Biomonitoring of tropospheric ozone phytotoxicity in rural catalonia. Atmospheric Environment 37(1): 63-71.
• Scebba F, Pucciareli I, Soldatini GF, and Ranieri A. 2003. O3- induced changes in the antioxidant systems and their relationship to different degrees of susceptibility of two clover species. Plant Science 165: 583-593.
• Schraudner M, Moeder W, Wiese C, Van Camp W, Inze D, Langebartels and Sandermann HJr. 1998. Ozone- induced oxidative burst in the ozone biomonitor plant, tobacco Bel W3. Plant Journal 16: 235-245.
• Seber GAF. 1982. Linear Hypothesis: A General Theory. Charles Griffin and Company Ltd, London.
• Sharma YE. and Davis KR. 1997. The effects of ozone on antioxidant responses in plants. Free Radical Biology & Medicine 23: 480-488.
• Stanners D, and Bourdeau P. 1995. Europe's environment. The Dobris assessment. Copenhagen European Environment Agency, 5547-551.
• Van Camp W, Willekens H, Bowler C, Van Montagu M, Inzé D, Reupold-popp R, Sandermann HJr, and Langebartels C. 1994 Elevated levels of superoxide dismutase protect transgenic plants against ozone damage. Biotechniques 12: 165-168.
• VDi Verein Deutscher ingenieure 2000. Biologische Messverfahren zur Ermittlung und Beurteilung der Wirkung von Luftverunreinigungen auf Pflanzen (Bioindikation). Ermittlung und Beurteilung der phytotoxischen Wirkung von Ozon und andren Photooxidantien. Verfahren der standardisierten TabakExposition. VDI-Guideline 3957 Part 6 (Draft).
• Wohlgemuth H, Mittelstrass K, Kschieschan S, Bender J, Weigel H-J, Overmyer K, Kangasjärvi J, Sandermann H, and Langebartels C. 2002. Activation of an oxidative burst is a general feature of sensitive plants exposed to the air pollutant ozone. Plant Cell and Environment 25: 717-726.