Cytokine mRNA expression and IFN-gamma production in nematode resistant and susceptible line lambs artificially infected with gastro-intestinal nematodes

• Autorzy: Pernthaner A , Vlassoff A , Douch P G C , Maass D R
• Języki publikacji: EN

Abstrakty

EN

The mRNA expression of the cytokines interleukin (IL)-2, IL-4 and interferon (IFN)-γ and the production of IFN-γ was examined in mesenteric lymph node cells (MLNC) and CD4⁺ enriched cells populations from resistant and susceptible line lambs by use of reverse transcriptase-polymerase chain reactions (RT-PCR) and ELISA. MLNC obtained from lambs that were previously infected with a mixed gastro-intestinal nematode population were stimulated with Con A or Trichostrongylus colubriformis specific antigen. Four weeks after infection MLNC from both line lambs expressed high levels of mRNA coding for IL-2, IL-4 and IFN-γ. MLNC from resistant line lambs when stimulated with antigen for one day had higher mRNA expression of IL-2 and IFN-γ and after three days of culture had higher levels of IL-4 mRNA than MLNC from susceptible line lambs. Antigen stimulated MLNC of resistant line lambs had a higher IFN-γ production than those from susceptible line lambs. These results indicate that in resistant line lambs the secondary in vitro responses of cytokine mRNA and IFN-γ production to nematode specific antigen differs from those of susceptible line lambs.

Słowa kluczowe

EN

infection; eosinophil; gastrointestinal nematode; susceptible line lamb; mesenteric lymph node cell; Trichostrongylus colubriformis; mRNA expression; mRNA coding; in vitro; intracellular pathogen; interleukin; antigen; cytokine; interferon; nematode; polymerase chain reaction; immune response

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1997
• Tom: 42
• Numer: 1
• Opis fizyczny: p.55-61

Twórcy

autor

Pernthaner A

• II Med. Clinic, Veterinary University Vienna, Josef-Baumanngasse 1, A-1210 Vienna, Austria

autor

Vlassoff A

• Wallaceville Animal Research Centre, AgResearch, P.O. Box 40063, Upper Hutt, New Zealand

autor

Douch P G C

• Wallaceville Animal Research Centre, AgResearch, P.O. Box 40063, Upper Hutt, New Zealand

autor

Maass D R

• Wallaceville Animal Research Centre, AgResearch, P.O. Box 40063, Upper Hutt, New Zealand

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