Antimicrobial activity of glucoprotamin-containing disinfectants

• Autorzy: Tyski S , Grzybowska W. , Grzeszczuk S. , Leszczynski P. , Staniszewska M. , Rohm-Rodowald E. , Jakimiak B.
• Języki publikacji: EN

Abstrakty

EN

Introduction of a new antimicrobial agent as a drug - for treatment of infections or as a disinfectant and antiseptic, may result in the occurrence of resistance mechanisms against this agent among microorganisms. Two disinfectants of different composition - Incidin Plus for surface disinfection and Sekusept Plus for medical devices disinfection, both containing glucoprotamin as the active substance, were investigated in this study in order to analyze their antimicrobial activity. Standard bacterial and fungal strains recommended by European Standards, established by European Standardization Committee for testing bactericidal and fungicidal activity of chemical disinfectants were used in the study. Furthermore, 60 clinical bacterial strains with different susceptibility to antibiotics and chemotherapeutics, mostly multiresistant, isolated from different specimens from hospitalized patients were analyzed. In addition, 184 fungal clinical strains isolated from hospitalized patients and outpatients were also included in this study. Antimicrobial activity was evaluated according to EN 1040:2005 - using bacterial strains and according to EN 1275:2005 - using fungal strains. Glucoprotamin proved to be a very effective and rapidly acting bactericidal and fungicidal agent. Low concentration of glucoprotamin - 0.5% showed to be very effective (1 min) against clinical bacterial isolates. Incidin Plus was also very effective (5 min) against clinical fungal isolates.

Słowa kluczowe

EN

chemical disinfectant; disinfectant; fungicidal activity; Sekusept Plus drug; treatment; antimicrobial activity; hospital infection; glucoprotamine; infection; active substance; new antimicrobial agent; Incidin Plus drug

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 4
• Opis fizyczny: p.347-353,fig.,ref.

Twórcy

autor

Tyski S

• National Medicines Institute, Chelmska 30/34, 00-725 Warsaw, Poland

autor

Grzybowska W.

autor

Grzeszczuk S.

autor

Leszczynski P.

autor

Staniszewska M.

autor

Rohm-Rodowald E.

autor

Jakimiak B.

Bibliografia

• Allen G. 2004. Bacteria bound to cloth; glucoprotamin; toluidine blue O; surgical helmets versus filtering masks. AORN Journal 80: 333-337.
• CLSI - Clinical and Laboratory Standard Institute. 2006. Performance standards for antimicrobial disk susceptibility tests, 9th edn, Approved Standard M2-A9. Wayne, PA.
• Coia J.E., G.J. Duckworth, D.L Edwards, M. Farrington, C. Fry, H. Humphreys, C. Mallaghan and D.R. Tucker. 2006. Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities. J. Hosp. Infect. 63S: S1-S44.
• Cookson B.D., M.B. Macrae, S.P. Barrett, D.F. Brown, C. Chadwick, G.L. French, P. Hateley, I.K. Hosein and J.J. Wade. 2005. Guidelines for the control of glycopeptide-resistant enterococci in hospitals. J. Hosp. Infect. 63: 6-21.
• Disch K. 1992. Glucoprotamin - a new antimicrobial substance. Hyg. Med. 17: 529-534.
• Disch K. 1994. Glucoprotamin - a new antimicrobial substance. Zbl. Hyg. 195: 357-365.
• EN 1040. 2005. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1). Brussels: European Committee for Standardization.
• EN 13727. 2003. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants for instruments used in the medical area - Test method and requirements (phase 2, step 1). Brussels: European Committee for Standardisation.
• EN 1275. 2005. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1). Brussels: European Committee for Standardisation.
• McDonnell G. and A.D. Russell. 1999. Antiseptics and disinfectants: activity, action, and resistance. Clin. Microbiol. Rev. 12: 147-179.
• Meyer B. and C. Kluin. 1999. Efficacy of glucoprotamin containing disinfectants against different species of atypical Mycobacteria. J. Hosp. Infect. 42: 151-154.
• Meyer B. 2006. Does microbial resistance to biocides create a hazard to food hygiene? Int. J. Food Microbiol. 112: 275-279.
• Tyski S., M. Staniszewska, B. Jakimiak, P. Leszczyński, W. Grzybowska and M. Łuczak. 2006. Efficacy of glucoprotamin containing disinfectant, against different bacterial and fungal clinical isolates. J. Hosp. Infect. 64(S1): S36.
• Tyski S., W. Grzybowska, S. Grzeszczuk, M. Staniszewska, B. Jakimiak and E. Röhm-Rodowald. 2007. Antimicrobial activity of glucoprotamin containing disinfectants. J. Infect. Control. 3(1S): 40.
• Von Rheinbaben F. and B.Meyer. 2008. Glucoprotamin. In: Kramer A., Assadin O. (eds). Wallhaufiers Praxis der Sterilisation, Desinfektion, Antiseptik und Konservierung, 6th edn. Stuttgart, New York: Georg Thieme Verlag KG: 786-787.
• Widmer A.F. and R. Frei. 2003. Antimicrobial activity of glucoprotamin: a clinical study of a new disinfectant for instruments. J. Control Hosp. Epidemiol. 24: 762-764.