Diagnosis of bovine leukemia virus [BLV] infection in newborn calves by use of PCR

• Autorzy: Kuzmak J , Kozaczynska B. , Bicka L.
• Języki publikacji: EN

Abstrakty

EN

The applicability of the polymerase chain reaction (PCR) for the detection of proviral DNA of BLV in newborn calves was tested. Out of 22 calves from BLV infected mothers, studied during first 3 weeks after birth, 13 were serologically positive by ELISA. However, only 3 calves were positive for BL V provirus in their blood leukocytes’ DNA. Since the serological test can not discriminate active infection from colostral immunity, detection of proviral DNA by PCR could be recommended for early diagnosis of BLV in newborn calves.

Słowa kluczowe

EN

newborn calf; enzootic bovine leukosis; calf; infection; diagnosis; provirus; bovine leukemia virus; polymerase chain reaction; detection

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1999
• Tom: 43
• Numer: 2
• Opis fizyczny: p.125-131,fig.

Twórcy

autor

Kuzmak J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kozaczynska B.

autor

Bicka L.

Bibliografia

• 1. Agresti A., Ponti W., Rocchi M., Moneveri R., Marozzi A., Cavalleri, Peri E., Poli G., Ginelli E.: Am. J. Vet. Res. 54, 378, 1993.
• 2. Burkhardt H., Muller M., Rosenthal S., Wittmann W.: Mh. Vet. Med. 43, 702, 1988.
• 3. Deuter H., Walther K., Pannwitz S.: Mh.Vet. Med. 43, 41, 1988.
• 4. Ferrer J. Piper C.: Cancer Res. 41, 4906, 1981.
• 5. Gareiss G., Wachter K., Wittmann W., Pitzschke H.: Mh. Vet. Med. 41, 801, 1986.
• 6. Gaudi S., Ponti W., Agresti A., Meneveri R., Malcovati M., Bonizzi. L., Poli, Amato A., Ginelli E.: Molec. Cell. Probes 4, 163, 1990.
• 7. Klimentowski S.: Medycyna Wet. 47, 345, 1991.
• 8. Kono Y., Sentsui H., Arai K., Fujigaki A., Enomoto C., lwasaki H., Ishida H.: Jap. J. Vet. Sci. 45, 453, 1983.
• 9. Lassauzet M., Johnson W., Turmond M., Picanso.: Prev. Vet. Med. 9, 45, 1990.
• 10. Meszaros J., Antal T., Polner A., Sumeghy L., Szabo I., Vajda G.: Acta Vet. Hungar. 42, 421, 1994.
• 11. Otachel-Hawranek J., Boruta.: Medycyna Wet. 46, 392, 1990.
• 12. Sagata N., YasunagaT., Tsuzuku-Kawamura J., Ohishi K., Ogawa Y., Ikawa Y.: Proc. Natl. Acad. Sci. USA. 82, 677, 1985.
• 13. Stear M., Dimmock C., Newman M., Nicholas F.: Animal Genet. 19, 151, 1988.
• 14. Straub O.: Fifth Inter. Sym. Bovine Leukosis, Tübingen. 258-264, 1982.
• 15. Thurmond M., Carter L., Puhr D., Burridge M.: Am. J. Vet. Res. 43, 1152, 1982.
• 16. Van Der Maaten M., Miller J., Schmerr J.: Am. J. Vet. Res. 42, 1498, 1981