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2006 | 62 | 03 |

Tytuł artykułu

Pierwszy przypadek poodsadzeniowego wielonarzadowego zespolu wyniszczajacego swin w Polsce

Warianty tytułu

EN
First report of postweaning multisystemic wasting syndrome in pigs in Poland

Języki publikacji

PL

Abstrakty

EN
The present study describes first clinically detected and confirmed by laboratory methods case of PMWS in Poland. The investigation was carried out in a large farrow-to-finish farm producing 40 000 fatteners per year. In the spring of 2000, growth retardation, wasting and respiratory disorders affected 20% of weaners and 30% of fatteners. Nineteen weaners and 11 fatteners with different degrees of wasting, respiratory disorders, skin paleness and hemorrhagic skin lesions (fatteners only) were necropsied. Post-mortem examination revealed the presence of inflammatory signs in lymphoid tissues, enlarged lymph nodes, non-collapsed lungs, enlarged livers, enlarged and pale kidneys with generalized cortical petechiae. Sections of lymph nodes were submitted for histopathological examination and in situ hybridization. Moreover, virus isolation in PK15 cell line was attempted. Histopathological analysis of lymph nodes revealed different degrees of lymphatic depletion and histiocitic infiltrations, giant cells and intracytoplasmic inclusion bodies. In situ hybridization with the use of a specific for PCV2 digoxigenin labelled DNA probe confirmed the abundant presence of PCV2 DNA within lesions in lymph nodes. A peroxidase assay indicated PCV2 replication in the inoculated cell line. Gross and histopathological lesions and the presence of PCV2 antigens in lymphoid tissues confirmed the presence of PMWS in a Polish swine herd. Clinical signs and laboratory examinations indicated that PDNS and PMWS coexisted in the herd. This suggests that PCV2 could be an important factor in an aetiology of PDNS.

Wydawca

-

Rocznik

Tom

62

Numer

03

Opis fizyczny

s.297-301,fot.,tab.,bibliogr.

Twórcy

autor
  • Panstwowy Instytut Weterynaryjny-Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy
autor
autor
autor

Bibliografia

  • 1. Allan G. M., Kennedy S., McNeilly F., Foster J. C., Ellis J. A., Krakowka S. J., Meehan B. M., Adair B. M.: Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J. Comp. Pathol. 1999, 121, 1-11.
  • 2. Allan G. M., McNeilly F., Ellis J., Krakowka S., Botner A., McCullough K., Nauwynck H., Kennedy S., Meehan B., Charreyre C.: PMWS: experimental model and co-infections. Vet. Microbiol. 2004, 98, 165-168.
  • 3. Allan G. M., McNeilly F., Ellis J., Krakowka S., Meehan B., McNair I., Walker I., Kennedy S.: Experimental infection of colostrum deprived piglets with porcine circovirus type 2 (PCV-2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV-2 replication. Arch. Virol. 2000, 145, 2421-2429.
  • 4. Chae C.: Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet. J. 2004, 168 (1), 41-49.
  • 5. Ellis J., Clark E., Haines D., West K., Krakowka S., Kennedy S., Allan G. M.: Porcine circovirus-2 and concurrent infections in the field. Vet. Microbiol. 2004, 98, 159-163.
  • 6. Fenaux M., Halbur P. G., Haqshenas G., Royer R., Thomas P., Nawagitgul P., Gill M., Toth T. E., Meng X. J.: Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J. Virol. 2002, 76 (2), 541-551.
  • 7. Gresham A., Allan G. M., McNeilly F., Kennedy S.: Links between postweaning multisystemic wasting syndrome and porcine dermatitis nephropathy syndrome. Pig J. 2001, 155-159.
  • 8. Hasslung F., Wallgren P., Ladekjaer-Hansen A. S., Botner A., Nielsen J., Wattrang E., Allan G. M., McNeilly F., Ellis J., Timmusk S., Belak K., Segall T., Melin L., Berg M., Fossum C.: Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2. Vet. Microbiol. 2005, 106 (1-2), 49-60.
  • 9. Harding J. C. S.: The clinical expression and emergence of porcine circovirus 2. Vet. Microbiol. 2004, 98, 131-135.
  • 10. Harms P. A., Sorden S. D., Halbur P. G., Bolin S. R., Lager K. M., Morozov I., Paul P. S.: Experimental reproduction of severe disease in CD/CD pigs concurrently infected with the 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet. Pathol. 2001, 38, 528-539.
  • 11. Kennedy S., Moffett D., McNeilly F., Meehan B., Ellis J., Krakowka S., Allan G. M.: Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine parvovirus. J. Comp. Pathol. 2000, 122, 9-24.
  • 12. Kim J., Chung H.-K., Chae C.: Association of porcine circovirus 2 with porcine respiratory disease complex. Vet. J. 2003, 166 (3), 251-256.
  • 13. Krakowka S., Ellis J., McNeilly F., Ringler S., Rings D. M., Allan G.: Activation of the immune system is the pivotal event in the production of wasting disease in pigs infected with PCV2. Vet. Pathol. 2001, 38, 31-42.
  • 14. Krakowka S., Ellis J. A., Meehan B., Kennedy S., McNeilly F., Allan G.: Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet. Pathol. 2000, 37, 254-263.
  • 15. Kyriakis S. C., Saoulidis K., Lekkas S., Miliotis Ch. C., Papoutsis P. A., Kennedy S.: The Effects of Immuno-modulation on the Clinical and Pathological Expression of Postweaning Multisystemic Wasting Syndrome. J. Comp. Path. 2001, 126, 38-46.
  • 16. Ladekjaer-Mikkelsen A.-S., Nielsen J., Stadejek T., Storgaard T., Krakowka S., Ellis J., McNeilly F., Allan G., Botner A.: Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Vet. Microbiol. 2002, 89, 97-114.
  • 17. Meehan B. M., McNeilly F., McNair I., McNair I., Walker I., Ellis J. A., Krakowka S., Allan G. M.: Isolation and characterisation of porcine circovirus 2 from cases of sow abortions and porcine dermatitis and nephropathy syndrome Arch. Virol. 2001, 146, 1-8.
  • 18. Pejsak Z., Kołodziejczyk P., Kozaczyński W., Stadejek T., Lipowski A., Roszkowski J.: Przypadek zespołu skórno-nerkowego w wielkotowarowej fermie świń. Medycyna Wet. 2001, 57, 591-594.
  • 19. Quintana J., Segalés J., Rosell C., Casamiglia M., Rodríguez-Arrioja G. M., Chianini F., Folch M., Maldonado J., Canal M., Plana-Durán J.: Clinical and pathological observations on pigs with postweaning multisystemic wasting syndrome. Vet. Rec. 2001, 149, 357-361.
  • 20. Rodríguez-Arrioja G. M., Ségales J., Balasch M., Rosell C., Quintana J., Folch J. M., Plana-Durán J., Mankertz A., Domingo M.: Serum antibodies to porcine circovirus type 1 nad type 2 in pigs with and without PMWS. Vet. Rec. 2000, 146, 762-764.
  • 21. Rosell C., Segales J., Ramos-Vara J. A., Folch J. M., Rodriguez-Arrioja G. M., Duran C. O., Balash M., Plana-Duran J., Domingo M.: Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Vet. Rec. 2000, 146, 40-43.
  • 22. Rosell C., Segalés J., Plana-Durán J., Balasch M., Rodríguez-Arrioja G. M., Kennedy S., Alan G. M., McNeilly F., Latimer K. S., Domingo M.: Pathological, Immunohistochemical, and In-situ Hybridization Studies of Natural Cases of Postweaning Multisystemic Wasting Syndrome (PMWS) in Pigs. J. Comp. Path. 1999, 120, 59-78.
  • 23. Rovira A., Balash M., Segalés J., García L., Plana-Durán J., Rosell C., Ellebrok H., Markentz A., Domingo M.: Experimental inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus 2. J. Virol. 2002, 76, 3232-3239.
  • 24. Segalés J.: Praktyczne aspekty zwalczania PMWS. Suplement - Świnie. Mag. Wet. 2004, 42-44.
  • 25. Segalés J., Rosell C., Domingo M.: Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. Veterinary Microbiology 2004, 98, 137-149.
  • 26. Stadejek T.: Możliwości laboratoryjnego rozpoznawania poodsadzeniowego, wielonarządowego zespołu wyniszczającego świń. Suplement - Świnie, Mag. Wet. 2001, 76-78.
  • 27. Stadejek T.: Znaczenie zakażeń cirkowirusem świń typ 2 (PCV-2). Medycyna Wet. 2002, 58, 492-496.
  • 28. Wellenberg G. J., Stockhofe-Zurwieden N., de Jong M. F., Boersma W. J., Elbers A. R.: Excessive porcine circovirus type 2 antibody titres may trigger the development of porcine dermatitis and nephropathy syndrome: a case-control study. Vet. Microbiol. 2004, 99 (3-4), 203-214.

Typ dokumentu

Bibliografia

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