Changes in peroxidase activities and soluble proteins in strawberry varieties under salt-stress

• Autorzy: Gulen H , Turhan E. , Eris A.
• Języki publikacji: EN

Abstrakty

EN

Effects of salt stress on the activity of peroxidase (PRX) isozyme and leaf proteins were studied in the three strawberry varieties, Camarosa, Tioga and Chandler. Plants were watered with modified 1/3 Hoagland nutrient solution containing 0 (control), 8.5, 17.0 and 34.0 mM NaCl for 30 days fallowing 20 days acclimation. PRX activity was assayed in leaf extracts and PRX profiles of control and salt treated plants were performed by native polyacrylamide gel electrophoresis (PAGE).During the salt stress Tioga and Camarosa tolerated the cellular damage with less electrolyte leakage while Chandler exhibited a severe cellular damage with the highest (87.5 % in 34.0 mM NaCl treatment) electrolyte leakage. Total soluble protein content was decreased by salinity in Tioga (from 11.35 to 5.86 mgg-1DW) and Chandler (from 9.35 to 3.90 mgg-1DW) while it was almost unchanged in Camarosa. Salt stress increased total and specific PRX activity as compared with the control in all the varieties. In native PAGE, one basic isoperoxidase band (Rf=0.27) was observed commonly with different band intentity in all the treatments. Therefore, it might be associated with lignification and recovery of cell membrane damage in strawberry plants under salt stress.

Słowa kluczowe

EN

leaf protein; peroxidase activity; salt stress; plant physiology; strawberry; protein; peroxidase

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2006
• Tom: 28
• Numer: 2
• Opis fizyczny: p.109-116,fig.,ref.

Twórcy

autor

Gulen H

• Uludag University, Gorukle, 16059 Bursa, Turkey

autor

Turhan E.

autor

Eris A.

Bibliografia

• Allen R. 1995. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol., 107:1049-1054.
• Arora R., Wisniewski M.E., Scorza R. 1992. Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunuspersica L. Batsch). I Seasonal changes in cold hardiness and polypeptides of bark and xylem tissues. Plant Physiol., 99: 1562-1568.
• Arora R., Wisniewski M.E. 1994. Cold acclimation in genetically related (sibling) deciduous and evergreen peach: A 60-kDa bark protein in cold-acclimated tissue of peach is heat-stable and related to the dehydrin family of proteins. Plant Physiol., 105: 95-101.
• Arora R., Rowland L.J., Panta G.R. 1997. Chill-responsive dehydrins in blueberry: Are they associated with cold hardiness or dormancy transitions?. Physiol. Plant., 101: 8-16.
• Arora R., Pitchay D.S., Bearce B.C. 1998. Water-stress induced heat tolerance in geranium leaf tissues: A possible linkage through stress proteins?. Physiol. Plant., 103: 24-34.
• Arshi A., Abdin M.Z., Iqbal M. 2002. Growth and metabolism of senna as affected by salt stress. Biol. Plant., 45: 295-298.
• Ashraf M., O’Leary W.J. 1999. Changes in soluble proteins in spring wheat stressed with sodium chloride. Biol. Plant., 42: 113-117.
• Awang Y.B., Atherton J.G., Taylor A.J. 1993. Salinity effects on strawberry plants grown in rockwool. I. Growth and leaf water relations. J. Hort. Sci., 68: 783-790.
• Barrosa M.C., Alvarez C.E. 1997. Toxicity symptoms and tolerance of strawberry to salinity in the irrigation water. Sci. Hort., 71: 177-188.
• Bellani L.M., Guarnieri M., Scialabba A. 2002. Differences in the activity and distribution of peroxidases from three different portions of germinating Brassica oleracea seeds. Physiol. Plant., 114: 102-108.
• Bohnert H.J., Nelson D.E., Jensen R.G. 1995. Adaptations to environmental stresses. Plant Cell, 7: 1099-1111.
• Bor M., Ozdemir F., Turkan I. 2003. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Sci., 164: 77-84.
• Bruce R.C., West C.A. 1989. Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Plant Physiol., 91: 889-897.
• Davis B.J. 1964. Disc electrophoresis. Method and application to human serum proteins. Ann. New York Acad. Sci., 121: 404-427.
• Dionisio-Sese M.L., Tobita S. 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci., 135: 1-9.
• Gaspar T., Penel C.L., Thorpe T., Greppin H. 1982. Peroxidases. A survey of their biochemical and physiological roles in higher plants. Universite de Geneve Press, Geneve.
• Greenway H., Munns R. 1980. Mechanisms of salt tolerance in non-halophytes. Ann. Rev. Plant Physiol., 31: 149-190.
• Grumet R. Isleib T.G., Hanson A.D. 1985. Genetic control of glycinebetaine level in barley. Crop Sci., 25: 618-622.
• Gulen H., Arora R., Kuden A., Krebs S.L., Postman J. 2002. Peroxidase isozyme profiles in compatible and incompatible pear-quince graft combinations. J. Amer. Soc. Hort. Sci., 127: 152-157.
• Gulen H., Eris A. 2003. Some physiological changes in strawberry (Fragaria x ananassa cv. Camarosa) plants under heat stress. J. Hort. Sci. Biotech., 78: 894-898.
• Hu C., Krol M., van Huystee R.B. 1989. Biosynthesis and localization of peanut peroxidases. A comparison of the cationic and the anionic isozymes. J. Plant Physiol., 135: 391.
• Lopez F. Vansuyt F., casse-Delbart P., Fourcroy P. 1996. Ascorbate peroxidase activity, not the mRNA level, is enhanced in salt-stressed Raphanus sativus plants. Physiol. Plant., 97: 13-20.
• Manganaris A.G., Alston F.H. 1992. Inheritance and linkage re-a-ionships of peroxidase isozymes in apple. Theoret. App. Genet., 83: 392-399.
• Meloni D.A., Oliva M.A., Martinez C.A., Cambraia J. 2003. Phytosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ. Exp. Botany, 49: 69-76.
• Mittal R., Dubey R.S. 1991. Behaviour of peroxidases in rice: changes in enzyme activity and isoforms in relation to salt toferance. Plant Physiol. Biochem., 29: 31-40.
• Morant-Avice A., Pradier E., Houchi R. 1998. Osmotic adjustment in triticales grown in presence of NaCl. Biol. Plant., 41: 227-234.
• Muthukumarasamy M., Gupta S.D., Panneerselvam R. 2000. Enhancement of peroxidase, polyphenol oxidase and superoxide dismutase activities by triadimefon in NaCl stressed Raphanus sativus L. Biol. Plant., 43: 317-320.
• Pareek A., Singla S.L., Grover A. 1997. Salt responsive proteins/genes in crop plants. In: Strategies For Improvmg Salt tolerance in Higher Plants, ed. by P.K. Jaiwal, R.P. Singh, A. Gulati., Oxford and IBH Publ. Co., New Delhi: 365-391.
• Premachandra G.S., Saneoka H., Fujita K., Ogata S. 1992. Leaf water relations, osmotic adjustment, cell membrane stability, epicuticular wax load and growth as affected by increasing water deficits in Sorghum. J. Exp. Bot., 43: 1569-1576.
• Qasim M., Ashraf M., Ashraf M.Y., Rehman S.U., Rha E.S. 2003. Salt-induced changes in two canoa cultivars differing in salt tolerance. Biol. Plant., 46: 629-632.
• Reisfeld R.A., Lewis U.J., Williams D.E. 1962. Disc electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature, 195: 281-283.
• Reuveni R.M., Shimoni M., Karchi Z., Kue J. 1992. Peroxidase activity as a biochemical marker for resistance of muskmelon (Cucumis melo) to Pseudoperonospora cubensis. Phytopathology, 82: 749-753.
• Rodriguez A., Pina D.G., Yelamos B., Castilloleon J. J., Zhadan G.G., Villar E., Gavilanes F., Roig M.G., Sakharov I.Y., Shnyrov V.L. 2002. Thermal stability of peroxidase from the African palm tree Eaeis guineeensis. Eur. J. Biochem., 269: 2584-2590.
• Ros-Barcelo A., Pomar F., Ferrer M.A., Martinez P., Ballesta M.C., Pedreno M.A. 2002. In situ characterization of a NO-sensitive peroxidase in lignifying xylem of Zinnia elegans. Physiol. Plant., 114: 33-40.
• Rout N.P., Shaw B.P. 2001. Salt tolerance in aquatic macrophytes: possible involment of antioxidative enzymes. Plant Sci., 160: 415-423.
• Screenivasulu N., Ramanjulu S., Ramachandra-Kini K., Prakash H.S., Shekar-Shetty H., Savithri H.S., Sudhakar C. 1999. Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail miliet with differential salt tolerfance. Plant Sci., 141: 1-9.
• Serrano R. 1996. Salt tolerance in plants and microorganisms: toxicity targets and defense responses. Inter. Rev. Cytology, 165: 1-52.
• Singh N.K., Bracken C.A., Hasegawa P.M., Handa A.K., Buckel S., Hermodson M.A., Pfankoch F., Regnier F.E., Bressan R.A. 1987. Characterization of osmotin. A thaumatin-like protein associated with osmotic adjustment in plant cells. Plant Physiol., 85: 529-536.
• Turhan E. 2002. Researches on salt resistance physiology of strawberries grown in different media. (In Turkish with English summary). Ph.D. Thesis, Uludag Univ. Inst. Natural Sci.,Bursa, Turkey.
• Turhan E., Eris A. 2004. Effects of sodium chloride applications and different growth media on ionic composition in strawberry plant. J. Plant Nutrit., 27: 1653-1665.
• van Huystee R.B. 1987. Some molecular aspects of plant peroxidases biosynthetic studies. Ann. Rev. Plant Physiol., 38: 205-219.
• van Huystee R.B., Sun Y., Lige B. 2002. A retrospective look at the cationic peanut peroxidase structure. Crit. Rev.Biotech., 22: 335-354.
• Wendel J.F., Weeden N.F. 1989. Visualization and interpretation of plant isozymes. In: Isozymes in Plant Biology, ed. by D.E. Soltis, P.S. Soltis, Dioscorides Pres, Portland, Oregon, 5-44.
• Yoshida K., Kaothien P., Matsui T., Kawaoka A., Shinmyo A. 2002. Molecular biology and application of plant peroxidase genes. App. Microb. Biotech., 60: 665-670.