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2008 | 64 | 06 |

Tytuł artykułu

Cechy fenotypowe i genotypowe szczepow Clostridium perfringens izolowanych od prosiat ssacych

Autorzy

Warianty tytułu

EN
Evaluation of phenotypic and genotypic features of Clostridium perfringens strains isolated from suckling piglets

Języki publikacji

PL

Abstrakty

EN
The aim of present study was to determine α and β toxin expression as well as the prevalence of α, β, ε, ι, β2 toxin genes and the enterotoxin gene of Clostridium perfringens strains isolated from suckling piglets with diarrhea. Rectal swabs, feces samples and intestine sections originating from 15 farms were examined during the study. All isolated through ELISA Cl. peringen strains demonstrated β toxin expression and none of them demonstrated β toxin. All isolates were positive for the β and negative for the β, ε, ι and enterotoxin gene, implying that only non-enterotoxigenic type A strains were detected. Over 41% of the isolates demonstrated β2 toxin gene. The β2 positive strains were isolated from samples originating from 9 farms. The lack of type C strains among isolates collected during the study was also noted, as well as the different intensity of pathological changes in the intestines of piglets from which β2 positive strains were isolated.

Wydawca

-

Rocznik

Tom

64

Numer

06

Opis fizyczny

s.791-795,fot.,tab.,bibliogr.

Twórcy

autor
  • Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy
autor

Bibliografia

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  • 11. Garmory H. S., Chanter N., French N. P., Bueschel D., Songer J. D., Tiball R. W.: Occurrence of Clostridium perfringens β2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol. Infect. 2000, 124, 61-67.
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  • 16. Hunter S. E. C., Brown E., Oyston P. C. F., Sakurai J., Titball R. W.: Molecular genetic analysis of beta-toxin of Clostridium perfringens reveals sequence homology with alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus. Infect. Immun. 1993, 61, 3958-3965.
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Typ dokumentu

Bibliografia

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