Inhibitors of benzamidine type influence the virulence properties of Porphyromonas gingivalis strains

• Autorzy: Eick S. , Pfister W. , Sturzebecher U. , Jarema S. , Sturzebecher J.
• Języki publikacji: EN

Abstrakty

EN

Synthetic inhibitors of benzamidine type have been found to have inhibiting effects on arginine specific cysteine proteinases of P. gingivalis. The purpose of our study was to assess the effects of these inhibitors on the virulence properties of two P. gingivalis strains, the reference strain ATCC 33277 and JH16-1, a clinical isolate ob­tained from a patient with severe periodontitis. The inhibitors tested were pentami- dine, benzamidine, three bis-benzamidine derivatives with a pentamidine-related structure, one bis-benzamidine derivative with another structure, and one arginine derivative as a negative control, each in the concentrations of 2 uM and 20 uM. As vir­ulence criteria the following parameters were determined: arginine-specific amidolytic activity, growth inhibition, hemagglutination of sheep erythrocytes, adher­ence to KB cells and immuno-phagocytosis including intracellular killing. Pentami- dine and the bis-benzamidine derivatives with pentamidine-related structure showed the most remarkable effects on reduction of amidolytic activity by 35%, growth inhibi­tion and reduced hemagglutination. Except for the arginine derivative all other inhibi­tors tested enhanced the phagocytosis capacities of granulocytes. No clear influence of the inhibitors on adherence of P. gingivalis to KB cells was seen. Although in vitro effects of the synthetic inhibitors of cysteine proteinases on virulence of P. gingivalis were observed further in vitro tests concerning immunomodulatory effects should be done before these substances are used for therapy in clinically controlled studies.

Słowa kluczowe

EN

adhesion; intracellular killing; phagocytosis; virulence property; synthetic inhibitor; growth inhibition; Porphyromonas gingivalis; cysteine proteinase; KB cell; benzamidine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: p.725-734,fig.

Twórcy

autor

Eick S.

• University Hospital of Jena, Semmelweistr.4, D-07740 Jena, Germany

autor

Pfister W.

autor

Sturzebecher U.

autor

Jarema S.

autor

Sturzebecher J.

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