EN
Restriction enzyme analysis has been done for the DNA Agille and III/3 strains of the BHV 1 virus, and variants selected from these strains after neutralization with polyclonal antibodies. It has been shown that the electrophoretic pattern of DNA of the 8/-2 variant which was selected from the Agille strain and which has had higher resistance to neutralization to antibodies has been identical to the electrophoretic pattern of the Agille parent strain. This may suggest that the higher resistance to neutralization of the 8/-2 variant has been a results of phenotype variability. No difference between eletrophoretic patterns of the III/3 strain and variants III/3D and III/3H has been discovered. Digestion with Hind III, Pst I, Bam HII, Eco RI enzymes differentiate the Agille and III/3 strain.