Murein-hydrolyzing activity of flagellin FlaA of Listeria monocytogenes

• Autorzy: Popowska M , Markiewicz Z.
• Języki publikacji: EN

Abstrakty

EN

In this preliminary report we show that a 29 kDa surface protein of Listeria monocytogenes EGD removed from cells with 4 M LiCl has peptidoglycan (murein) hydrolyzing activity, as revealed by zymographic analysis using Bacillus subtilis murein and heat-killed Micrococcus luteus cells casted in the gel. Following two-dimensional electrophoresis, the protein was electroblotted to PVDF membrane and its identity (FlaA) was revealed by sequencing. Peptidoglycan hydrolysing activity of FlaA purified by FPLC on Mono-S Sepharose against labelled Escherichia coli murein was demonstrated.

Słowa kluczowe

EN

autolysin; flagellin; Listeria monocytogenes; man; pathogen; food-borne infection; motility

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 4
• Opis fizyczny: p.237-241,fig.,ref.

Twórcy

autor

Popowska M

• Warsaw University, Miecznikowa 1, 02-096 Warsaw, Poland

autor

Markiewicz Z.

Bibliografia

• Cabanes D., O. Dussurget, P. Dehoux and P. Cossart. 2004. Auto, a surface associated autolysin of Listeria monocytogenes required for entry into eukaryotic cells and virulence. Mol Microbiol. 51: 1601-1614.
• Carroll S.A., T. Hain, U. Technow, A. Darji, P. Pashalidis, S.W. Joseph and T. Chakraborty. 2003. Identification and characterization of a peptidoglycan hydrolase, MurA, of Listeria monocytogenes, a muramidase needed for cell separation. J. Bacteriol. 185: 6801-6808.
• Copeland B.R., R.J. Richter and C.E. Furlong. 1982. Renaturation and identification of periplasmic protein in two-dimensional gels of Escherichia coli. J. Biol. Chem. 257: 15065-15071.
• Cossart P. and C. Kocks. 1994 The actin-based motility of the facultative intracellular pathogen Listeria monocytogenes. Mol. Microbiol. 13: 395-402.
• Cossart P. and M. Lecuit. 1998 Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: bacterial factors, cellular ligands and signaling. EMBO J. 17: 3797-3806.
• Dons L., O.F. Rasmussen and J.E. Olsen. 1992. Cloning and characterization of a gene encoding flagellin of Listeria monocytogenes. Mol. Microbiol. 6: 2919-2929.
• Dons L., E. Eriksson, Y. Jin, M.E. Rottenberg, K. Kristensson, C.N. Larsen, J. Bresciani and J.E. Olsen. 2004. Role of flagellin and the two-component CheA/CheY system of Listeria monocytogenes in host cell invasion and virulence. Infect. Immun. 72: 3237-3244.
• Eriksson E., L. Dons, A.G. Rothfuchs, P. Heldin, H. Wigzell and M.E. Rottenberg. 2003. CD44-regulated intracellular proliferation of Listeria monocytogenes. Infect Immun. 71: 4102-11.
• Foster S.J. 1992. Analysis of the autolysins of Bacillus subtilis 168 during vegetative growth and differentiation by using renaturing polyacrylamide gel electrophoresis. J. Bacteriol. 174: 464-470.
• Ghuysen J.-M., J. Lamolle-Brasseur, B. Joris and G.D. Shockman. 1994. Binding site-shaped repeated sequences of bacterial wall peptidoglycan hydrolases. FEBS Lett 342: 23-28.
• Grundling A., L.S. Burrack, H.G. Bouwer and D.E. Higgins. 2004. Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence. Proc. Natl. Acad. Sci. USA 101: 12318-12323.
• Heukeshoven J. and R. Dernick. 1988. Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyi sulfate gels. Electrophoresis. 9: 28-32.
• Hirano T., T. Minamino and R.M. Macnab. 2001. The role in flagellar rod assembly of the N-terminal domain of Salmonella FlgJ, a flagellum-speciflc muramidase. J. Mol. Biol. 312: 359-369.
• Hof H. 1984. Virulence of different strains of Listeria monocytogenes serovar 1/2a. Med. Microbiol. Immunol. (Berl). 173: 207-218.
• Hof H., T. Nichterlein and M. Kretschmar. 1997. Management of listeriosis. Clin. Microbiol. Rev. 10: 345-357.
• Joris B., S. Englebert, C.P. Chu, R. Kariyama, L. Daneo-Moore, G.D. Shockman and J.M. Ghuysen. 1992. Modular design of the Enterococcus hirae muramidase-2 and Streptococcus faecalis autolysin. FEMS Microbiol. Lett. 70: 257-264.
• Lazarevic V., P.B. Margot, B. Soldo and D. Karamata. 1992. Sequencing and analysis of the Bacillus subtilis lytRABC divergon: a regulatory unit encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and its modifier. J. Gen. Microbiol. 138: 1949-1961.
• Lenz L.L., S. Mohammadi, A. Geissler and D.A. Portnoy. 2003. SecA2-dependent secretion of autolytic enzymes promotes Listeria monocytogenes pathogenesis. Proc. Natl. Acad. Sci. USA 100: 12432-12437.
• McLaughlan M.N. and S.J. Foster. 1997. Characterisation of the peptidoglycan hydrolases of Listeria monocytogenes EGD. FEMS Microbiol. Lett. 152: 149-154
• Michel E., J. Menagaud, S. Galsworthy and P. Cossart. 1998. Characterization of a large motility gene cluster containing the cheR, motAB genes of Listeria monocytogenes and evidence that PrfA downregulates motility genes. FEMS Microbiol. Lett. 169: 341-347
• Milohanic E., R. Jonquiéres, P. Glaser, P. Dehoux, C. Jacquet, P. Berche, P. Cossart and J-L.Gaillard. 2004. Sequence and binding activity of the autolysin-adhesin Ami from epidemic Listeria monocytogenes 4b. Infect. Immun. 72: 4401-4409.
• Nambu T., T. Minamino, R.M. Macnab and K. Kutsukake. 1999. Peptidoglycan-hydrolyzing activity of the FlgJ protein, essential for flagellar rod formation in Salmonella typhimurium. J. Bacteriol. 181: 1555-1561.
• Rashid M.H., A. Kuroda and J. Sekiguchi. 1993. Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility. FEMS Microbiol. Lett. 112: 135-140.
• Schubert K., A.M. Bichlmaier, E. Mager, K. Wolff, G. Ruhland and F. Fiedler. 2000. P45, an extracellular 45 kDa protein of Listeria monocytogenes with similarity to protein p60 and exhibiting peptidoglycan lytic activity. Arch. Microbiol. 173: 21-28.
• Southwick F.S. and D.L. Purich, 1996. Intracellular pathogenesis of listeriosis. N Engl J Med. 334: 770-776.
• Viollier P.H. and L. Shapiro. 2003. A lytic transglycosylase homologue, PleA, is required for the assembly of pili and the flagellum at the Caulobacter crescentus cell pole. Mol. Microbiol. 49: 331-345.
• Way S.S., L.J. Thompson, J.E. Lopes, A.M. Hajjar, T.R. Kollmann, N.E. Freitag and C.B. Wilson. 2004. Characterization of flagellin expression and its role in Listeria monocytogenes infection and immunity. Cell. Microbiol. 6: 235-242.