High L-ascorbic acid content of diet modulates the non-specific immunity in rats

• Autorzy: Niemiec T , Sawosz E. , Grodzik M. , Chwalibog A.

Warianty tytułu

PL

Wysoki poziom kwasu L-askorbinowego w diecie moduluje nieswoista odpornosc szczurow

• Języki publikacji: EN

Abstrakty

EN

This study was designed to examine the effect of high dose of L-ascorbic acid (L-AA) as a dietary supplement for rats on the phagocytic activity and oxidative burst of non-specific immune cells. Thirty Wistar rats (225-245 g initial body weight) were divided into three groups fed diet supplemented with 0.0, 0.3, and 0.6% L-AA (0, 187 and 375 mg L-AA/kg feed) for 41 days. At the end of the experiment, blood samples were analysed for selected indicators of non-specific immunity. The application of 0.6% of LAA, as compared to the remaining two groups reduced phagocytic activity measured as a number of opsonized E. coli cells consumed by neutrophiles and monocytes. However, 0.6% of dietary AA supplementation led to increased phagocytic monocytes and neutrophiles percentage,which produced reactive oxygen species after stimulation with opsonized bacteria (E. coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP).These results indicate that megadose (0.6%) of dietary AA can reduce an oxygen-independent phagocytosis, but increase the number of neutrophiles and monocytes representing the oxygendependent mechanism of killing.

PL

Celem pracy była ocena wpływu wysokiej zawartości kwasu L-askorbinowego w diecie na aktywność fagocytarną i wybuch tlenowy neutrofili i monocytów krwi obwodowej szczurów. Zwierzęta trzech grup (po 10 osobników) żywiono przez 41 dni mieszanką półsyntetyczną o różnej zawartości (0,0; 0,3 i 0,6%)kwasu L-askorbinowego (L-AA). Poziom 0,3% L-AA w mieszance nie wpływał na aktywność badanych komórek odpornościowych. Poziom 0,6% L-AA w mieszance wpłynął na zwiększenie liczby neutrofili i monocytów z uruchomionymi mechanizmami wybuchu tlenowego, a zarazem spowodował zmniejszenie liczby bakterii sfagocytowanych przez te komórki. Uzyskane wyniki wskazują, że megadawka (0,6%) kwasu L-askorbinowego w mieszance może hamować beztlenowe mechanizmy zabijania drobnoustrojów w procesie fagocytozy, zwiększając zarazem liczbę neutrofili i monocytów z uruchomionymi procesami bakteriobójczymi.

Słowa kluczowe

EN

non-specific immunity; vitamin C; high content; L-ascorbic acid; diet; rat; phagocytic activity; feeding; immune response

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2009
• Tom: 27
• Numer: 2
• Opis fizyczny: p.149-157,ref.

Twórcy

autor

Niemiec T

• Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Sawosz E.

autor

Grodzik M.

autor

Chwalibog A.

Bibliografia

• AFANASEV I.B., 2006 – Competition between superoxide and hydrogen peroxide signalling in heterolytic enzymatic processes. Medical Hypotheses 66, 1125-1128.
• ANDREASEN C.B., FRANK D.E., 1999 – The effect of ascorbic acid on in vitro heterophile function. Avian Diseases 43, 656-663.
• APPELBERG R., 2007 – Neutrophiles and intracellular pathogens: beyond phagocytosis and killing.Trends in Microbiology 15, 87-92.
• AVIRAM I., FABER A., 1990 – A protein of neutrophile granules interferes with activation of NADPH oxidase in a cell-free system. Biochemical and Biophysical Research Communications 169, 198-202.
• BANHEGYI G., BRAUN L., CSALA M., PUSKAS F., MANDL J., 1997 – Ascorbate metabolism and its regulation in animals. Free Radical Biology & Medicine 23, 793-803.
• BARTSCH H, NAIR J., 2006 – Chronic inflammation and oxidative stress in the genesis and perpetuation of cancer: role of lipid peroxidation, DNA damage, and repair. Langenbeck’s Archives of Surgery 391, 499-510.
• BENNA J.E., DANG P.M., GAUDRY M., FAY M., MOREL F., HAKIM J., GOUGEROTPOCIDALO M. A., 1997 – Phosphorylation of the Respiratory Burst Oxidase Subunit p67phox during Human Neutrophile Activation. The Journal of Biological Chemistry 272, 17204-17208.
• BERGMAN M., SALMAN H., DJALDETTI M., FISH L., PUNSKY I., BESSLER H., 2004 – In vitro immune response of human peripheral blood cells to vitamins C and E. The Journal of Nutritional Biochemistry 15, 45-50.
• CIOCOIU M., LUPUSORU E.C., COLEV V., PADURARU O., 1998 – The involvement of vitamins C and E in changing the immune response. Revista Medico-Chirurgicală a Societătii de Medici şi Naturalişti din Iaşi 102, 93-96.
• COOKE M. S., EVANS M.D., PODMORE I. D., HERBERT K. E., MISTRY N., MISTRY P.,HICKENBOTHAM P. T., HUSSIENI A., GRIFFITHS H.R., LUNEC J., 1998 – Novel repair action of vitamin C upon in vivo oxidative DNA damage. FEBS Letters 363, 363-367.
• CUMMINS K.A., BRUNNER C.J., 1989 – Dietary ascorbic acid and immune response in dairy calves. Journal of Dairy Science 72, 129-134.
• DEL RIO M., RUEDAS G., MEDINA S., VICTOR V.M., DE LA FUENTE M., 1998 – Improvement by several antioxidants of macrophage function in vitro. Life Sciences 63, 871-881.
• EL-BENNA J., DANG P.M., GOUGEROT-POCIDALO M.A., ELBIM C., 2005 – Phagocyte NADPH oxidase: a multicomponent enzyme essential for host defenses. Archivum Immunologiae et Therapiae Experimentalis 53, 199-206.
• EO J., LEE K.J., 2008 – Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes. Fish & Shellfish Immunology 25, 611-616.
• GALLEY F., WEBSTER N.R., LAWLER P., SONI. N, SINGER M. (Eds)., 2004 – Critical Care Focus 10: Inflammation and Immunity. BMJ Books, London, UK.
• GERBER C.E., NIETHAMMER D., BRUCHELT G.,. 2002 – Differences in the accumulation of ascorbic acid in normal, myeloperoxidase deficient and NADPH-oxidase deficient granulocytes.International Journal for Vitamin and Nutrition Research 72, 251-256.
• GROSS W.B., 1992 – Effects of ascorbic acid on stress and disease in chickens. Avian Diseases 36,688-692.
• HOFFMANN-JAGIELSKA M., WINNICKA A., JAGIELSKI D., MICUŃ J., ŻMUDZKA M.,LECHOWSKI R., 2005 – Influence of naturally acquired feline leukaemia virus (FeLV) infection on phagocytic and oxidative burst activity of neutrophiles and monocytes of peripheral blood. Polish Journal of Veterinary Sciences 8, 93-97.
• KRAUSE R., PATRUTA S., DAXBOCK F., FLADERER P., BIEGELMAYER C., WENISCH C.,2001 – Effect of vitamin C on neutrophile function after high-intensity exercise. European Journal of Clinical Investigation 31, 258-263.
• LAGGNER H., PHILLIPP K., GOLDENBERG H., 2006 – Free zinc inhibits transport of vitamin C in differentiated HL-60 cells during respiratory burst. Free Radical Biology & Medicine 40, 436-443.
• LIU H., POPE R.M., 2004 – Phagocytes: mechanisms of inflammation and tissue destruction.Rheumatic Diseases Clinics of North America 30, 19-39.
• MAY R.C., MACHESKY L.M., 2001 – Phagocytosis and the actin cytoskeleton. Journal of Cell Science 114, 1061-1077.
• NIEMIEC T., SAWOSZ E., CHWALIBOG A., 2005 – Effect of high doses of L-ascorbic acid on the antioxidative/oxidative state in the rats. Journal of Animal and Feed Sciences 14, 553-556.
• NRC, 1995 – Nutrient Requirements of Laboratory Animals. National Academy Press, Washington,DC
• Operators Manual Phagotest Version 7/96, http://www.orpegen.com/share/phagotst.pdf
• Operators Manual Burstest Version 7/96, http://www.orpegen.com/share/bursttst.pdf
• PACLET M.H., BERTHIER S., KUHN L., GARIN J., MOREL F., 2007 – Regulation of phagocyte NADPH oxidase activity: identification of two cytochrome b558 activation states. The FASEB Journal 21, 1244-1255.
• PODMORE I.D., GRIFFITHS H.R., HERBERT K.E., MISTRY N., MISTRY P., LUNEC J., 1998 – Vitamin C exhibits pro-oxidant properties. Nature 392, 559.
• REHMAN A., COLLIS C.S, YANG M., KELLY M., DIPLOCK A.T., HALLIWELL B., 1998 – The effects of iron and vitamin C co-supplementation on oxidative damage to DNA in healthy volunteers.Biochemical and Biophysical Research Communications 246, 293-298.
• TAL T., SHARABANI M., AVIRAM I., MICHAELA S., IRIT A., 1998 – Cationic proteins of neutrophile azurophilic granules: protein-protein interaction and blockade of NADPH oxidase activation. Journal of Leukocyte Biology 63, 305-311.
• WU C.C., DORAIRAJAN T., LIN T.L., 2000 – Effect of ascorbic acid supplementation on the immune response of chickens vaccinated and challenged with infectious bursal disease virus.Veterinary Immunology and Immunopathology 74, 145-152.
• VICTOR V.V., GUAYERBAS N., PUERTO M., MEDINA S., DE LA FUENTE M., 2000 –Ascorbic acid modulates in vitro the function of macrophages from mice with endotoxic shock.Immunopharmacology 46, 89-101.