Canine skin tumours with adnexal differentiation: histopathology and immunohistochemistry

• Autorzy: Jasik A , Kozaczynski W. , Reichert M.
• Języki publikacji: EN

Abstrakty

EN

Thirty nine canine adnexal tumours were histologically and immunohistochemically analysed. They were classified as trichoblastomas, trichoepitheliomas, sebaceous adenomas and epitheliomas, hepatoid gland adenomas and epitheliomas, and aporcine adenocarcinomas. Moreover, hepatoid gland angioadenoma and angioepithelioma were recognised. Studies of follicular tumours revealed coexpression of cytokeratin MNF116 and LP34, but sebaceous and hepatoid gland tumours as well as apocrine adenocarcinomas showed differences in the expression of both cytokeratins. All the tumours were negative for vimentin except two hepatoid gland adenomas in which coexpression of vimentin and cytokeratin was observed. Positive reaction for neuron-specific enolase (NSE) was observed in follicular tumours, whereas expression of α-smooth muscle actin (α-SMA) was found only in apocrine adenocarcinomas. Moreover, the presence of NSE observed in hepatoid gland adenomas was considered as non-specific just as both glial fibrillary acidic protein in sebaceous gland tumours and hepatoid gland adenomas, and also S100 protein in sebaceous adenomas. All the examined tumours showed lack of expression of both desmin and factor FVIII. Thus, among the used antibodies, cytokeratins, vimentin, NSE, and α-SMA play a main role in the evaluation of canine adnexal histogenesis.

Słowa kluczowe

EN

animal disease; dog; skin tumour; adnexal tumour; histopathology; immunohistochemistry; adnexal differentiation; canine adnexal histogenesis

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 2
• Opis fizyczny: p.277-283,fig.,ref.

Twórcy

autor

Jasik A

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kozaczynski W.

autor

Reichert M.

Bibliografia

• 1. Chijiwa K., Uchida K., Tateyama S.: Immunohistochemical evaluation of canine peripheral nerve sheath tumors and other soft tissue sarcomas. Vet Pathol 2004, 41, 307-318.
• 2. Coindre J.M.: Immunohistochemistry in the diagnosis of soft tissue tumours. Histopathology 2003, 43, 1-16.
• 3. Collina G., Eusebi V., Capella C., Rosai J.: Merkel cell differentiation in trichoblastoma. Virchows Arch 1998, 433, 291-296.
• 4. Desnoyers M.M., Haines D.M., Searcy G.P.: Immunohistochemical detection of intermediate filament proteins in formalin fixed normal and neoplastic canine tissues. Can J Vet Res 1990, 54, 360-365.
• 5. Goldschmidt M.H., Dunstan R.W., Stannard A.A., von Tscharner C., Walder E.J., Yager J.A.: Histological classification of epithelial and melanocytic tumors of the skin of domestic animals. 2nd series, vol. III, Armed Forces Institute of Pathology, Washington D.C., 1998.
• 6. Iyer P.V., Leong A.S.: Poorly differentiated squamous cell carcinomas of the skin can express vimentin. J Cutan Pathol 1992, 19, 34-39.
• 7. Janes S.M., Lowell S., Hutter C.: Epidermal stem cells. J Pathol 2002, 197, 479-491.
• 8. Kato K., Uchida K., Nibe K., Tateyama S.: Immunohistochemical studies on cytokeratin 8 and 18 expression in canine cutaneous adnexus and their tumors. J Vet Med Sci 2007, 69, 233-239.
• 9. Konno A., Nagata M., Nanko H.: Immunohistochemical diagnosis of a Merkel cell tumor in a dog. Vet Pathol 1998, 35, 538-540.
• 10. Kozaki M., Nakamura Y., Iguchi M., Kano R., Watanabe S., Fujiwara K., Hasegawa A.: Immunohistochemical analysis of cytokeratin expresion in dog skin. J Vet Med Sci 2001, 63, 1-4.
• 11. Lohmann C.M., Solomon A.R.: Clinicopathologic variants of cutaneous squamous cell carcinoma. Adv Anat Pathol 2001, 8, 27-36.
• 12. Martín de las Mulas J., Espinosa de los Monteros A., Carrasco L., van Niel M., Fernández A.: Immunohistochemical distribution pattern of intermediate filament proteins in 50 feline neoplasma. Vet Pathol 1995, 32, 692-701.
• 13. Nibe K., Uchida K., Itoh T., Tateyama S.: A case of canine apocrine sweat gland adenoma, clear cell variant. Vet Pathol 2005, 42, 215-218.
• 14. Nielsen S.W.: Classification of tumors in dogs and cats. J Am Anim Hosp Assoc 1983, 19, 13-60.
• 15. Nielsen S.W., Aftosmis J.: Canine perianal gland tumors. J Am Vet Med Assoc 1964, 144, 127-135.
• 16. Niezabitowski A., Stachura J., Dubiel-Bigaj M.: Diagnostyka immunohistochemiczna nowotworów tkanek miękkich. Patol Pol 1990, 41, 28-37.
• 17. Rabanal R.H., Fondevila D.M., Montané V., Domingo M., Ferrer L.: Immunocytochemical diagnosis of skin tumours of the dog with special reference to undifferentiated types. Res Vet Sci 1989, 47, 129-133.
• 18. Schmitt F.C., Bacchi C.E.: S-100 protein; is it useful as a tumour marker in diagnostic immunocytochemistry? Histopathology 1989, 15, 281-288.
• 19. Schultz T., Hartschuh W.: Merkel cells are absent in basal cell carcinoma but frequently found in trichoblastoma. An immunohistochemical study. J Cutan Pathol 1997, 24, 14-24.
• 20. Shabadash S.A., Zelikina T.I.: Contradictions in modern data on the structure and function of dog hepatoid circumanal glands. Biol Bull 2002, 29, 229-237.
• 21. Shabadash S.A., Zelikina T.I.: Detection of hepatoid glands and distinctive features of the hepatoid acinus. Biol Bull 2002, 29, 559-567.
• 22. Sharif M.A.M.: Epidemiology of skin tumors entities according to the new WHO classification in dogs and cats. VVB, LAUFERSWEILER VERLAG, Giessen, 2006.
• 23. Stefansson K., Wollmann R., Jerkovic M.: S-100 protein in soft-tissue tumors derived from Schwann cells and melanocytes. Am J Pathol 1982, 106, 261-268.
• 24. Walter J.: A cytokeratin profile of canine epithelial skin tumours. J Comp Path 2000, 122, 278-287.
• 25. White S.D., Yager J.A.: Resident dendritic cells in the epidermis: Langerhans cells, Merkel cells and melanocytes. Vet Dermatol 1995, 6, 1-8.
• 26. Williamson M.M., Middleton D.J.: Cutanous soft tissue tumours in dogs: classification, differentiation and histogenesis. Vet Dermatol 1998, 9, 43-48.
• 27. Vos J.H., Van den Ingh T.S.G.A.M., Misdorp W., Ramaekers F.C.S., Van Mil F.N., De Neijs M.: An immunohistochemical study of canine tissues with vimentin, desmin, glial fibrillary acidic protein and neurofilament antisera. J Vet Med A 1989, 36, 561-575.
• 28. Vos J.H., Van den Ingh T.S.G.A.M., Ramaekers F.C.S., Molenbeek R.F., de Neijs M., van Mil F.N., Ivanyi D.: The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region. Vet Pathol 1993, 30, 352-361.
• 29. Vos J.H., Van den Ingh T.S.G.A.M., Ramaekers F.C.S., De Neijs M., van Mil F.N., Ivanyi D.: Keratin and vimentin distribution patterns in the epithelial structures of the canine anal region. Anat Rec 1992, 234, 391-398.