EN
The growth rate and the number of positive cultures of M. paratuberculosis on Nemoto’s, serum agar and Herrold’s media with the addition of mycobactins have been compared. The mycobactins were prepared from M. phlei, M. intracellulare, M. avium and M. tuberculosis. The suspensions of M. paratuberculosis culture or tissues, intestines and lymph nodes, collected from cattle affected with John’s disease were used for inoculation. The best results were obtained on Nemoto’s medium with the addition of a dried mass of M. phlei or M. paratuberculosis, strain TEPS.