Two wheat pre-tRNAsTyr containing introns and flanks are accurately and efficiently spliced in homologous wheat germ S23 extract. The initiation and termination sites upon in vitro transcription in HeLu cell extract have been estimated for both pre-tRNAs.
Adam Mickiewicz University, A.Fredry 10, 61-701 Poznan, Poland
Bibliografia
1. Sprinzl, M„ Dank, N., Nock. S. & SchCn. A. (1990) Compilation of tRNA and iRNA gene sequences. Nucleic Acids Res.. 19S. 2127 - 2171.
2. Beier, D., Stange, N.. Gross, H. J. & Beier, H. (1991) Nuclear tRNA1*' genes arc highly amplified at a single chromosomal site in the genome of Arabulopsis ihaliana. Mol. Gen. Genet., 225, 72 - 80.
3. Stange, N. & Beier, H. (1986) A gene for the major cytoplasmic tRNATyr from Nicotiana rustica contains a 13 nucleotides long intron. Nucleic Acids Res., 14, 8691.
4. Szweykowska-Kulińska, Z. & Beier, H. (1990) Nucleotide sequences of two nuclear tRNAlyr genes fioin Triticum ucslivum. Nucleic Acids Res., 18. 1894.
5. Szweykowska-Kulińska. Z. & Badźmierowska, A. (1992) Nucleotide sequence of a nuclear tRNATyT gene from Triticum aestivum. Plant Mol. Biol., 18, 1207 : 1208.
6. Stange. N„ Beier. D. & Beier. H. (1991) Expression of nuclear tRNAlyr genes from Arabidopsis thaliana in HeLa cell and wltcat germ extracts. Plant Mol. Biol., 16, 865 - 875.
7. Dignam, J. D., Lcbowife, R. M. & Rocdcr. R. G. (1983) Accurate transcripuon initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res., 11. 1475 - 1489.
8. Stange, N. & Beier. H. (1987) A cell-free plant extract for accurate pre-tRNA processing, splicing and modification. EMBO J., 6, 2811 - 2818.
9. Dingcrmann. T. & Ncikc, K. (1987) Primer extension analysis of tRNA gene transcripts synthesized in vitro and in vivo. Anal. Bwchem., 162,466 - 475.
10. Bogcnliagen. L). F\ & Brown. D. D. (1981) Nucleotide sequences in Xenopus 5S DNA for transcription termination. Cell, 24. 261 - 270.
11. Stange, N., Gross, H. J. & Beier, H. (1988) Wheat germ splicing cndonuclcasc is highly specific for plant tRNAs. F.MBO J., 7, 3823 - 3828.