Extended multiple-locus variable-number tandem-repeat analysis of Bacillus anthracis strains isolated in Poland

• Autorzy: Gierczynski R , Jakubczak A. , Jagielski M.
• Języki publikacji: EN

Abstrakty

EN

Twenty-one variable-number tandem-repeat (VNTR) marker loci were used for extended multiple locus VNTR analysis (MLVA) of 14 laboratory strains of Bacillus anthracis isolated in Poland and vaccine strain Sterne 34F2A. The extended MLVA (MLVA-21) distinguished six genotypes clustered in three main branches. Monomorphic branch 1 consisted of the vaccine strain and six isolates from distinct samples of a cow died from anthrax. This group also encompassed three haemolytic isolates of B. anthracis. Branches 2 and 3 were heterogeneous and consisted of five and three isolates of the phylogenetic lineages B2 and A1, respectively. MLVA-21 supported thesis on the anthrax agent heterogeneity in Poland. This study brought an additional evidence that haemolytic B. anthracis strains isolated in Poland are closely related to the vaccine strain Steme 34F2 and may together constitute the same sensu stricto strain. No epidemiological link could be however traced between both the vaccine and the haemolytic strains.

Słowa kluczowe

EN

isolation; geographic distribution; genotyping; Poland; Bacillus anthracis; bacteria; vaccine strain; hemolysis

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 1
• Opis fizyczny: p.3-7,fig.,ref.

Twórcy

autor

Gierczynski R

• National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Jakubczak A.

autor

Jagielski M.

Bibliografia

• Benson G. 1999. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acid Res. 27: 573-580.
• Brumlik M.J., U. Szymajda, D. Zakowska, X. Liang, R.J. Redkar, G. Patra and V.G. Del Vecchio. 2001. Use of long range repetitive element polymorphism-PCR to differentiate Bacillus anthracis strains. Applied Environ. Mirobiol. 67: 3021-3028.
• Ciammaruconi A., S. Grassi, R. De Santis, G. Faggioni, V. Pittiglio, R. D'Amelio, A. Carattoli, A. Cassone, G. Vergnaud and F. Lista. 2008. Fieldable genotyping of Bacillus anthracis and Yersinia pestis based on 25-loci Multi Locus VNTR Analysis. BMC Microbiol. 29: 8: 21 [http://www.biomedcentral.com/1471-2180/8/12].
• Easterday W.R., M.N. Van Ert, T.S. Simson, D.M. Wagner, L.J. Kenefic, Ch.J. Allender and P. Keim. 2005. Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. J. Clin. Microbiol. 43: 1995-1997.
• Fasanella A., M.N. Van Ert, S.A. Altamura, G. Garofolo, C. Buonavoglia, G. Leori, L. Huynh, S. Zanecki and P. Keim. 2005. Molecular diversity of Bacillus anthracis in Italy. J. Clin. Microbiol. 43: 98-3401.
• Fouet A., K.L. Smith, Ch. Keys, J. Vassaire, C. Le Doujet, M. Levy, M. Mock and P. Keim. 2002. Diversity among French Bacillus anthracis isolates. J. Clin. Microbiol. 40: 4732-4734.
• Gierczyński R., S. Kałużewski, A. Rakin, M. Jagielski, A. Zasada, A. Jakubczak, B. Borkowska-Opacka and W. Rastawicki. 2004. Intriguing diversity of Bacillus anthracis in eastern Poland -the molecular echoes of the past outbreaks. FEMS Microb. Lett. 239: 235-240.
• Gierczyński R., A.A. Zasada, N. Raddadi, M. Merabishvili, D.Daffonchio, W. Rastawicki and M. Jagielski. 2007. Specific Bacillus anthracis identification by plcR-targeted restriction site insertion-PCR (RSI-PCR) assay. FEMS Microbiol. Letters. 272: 55-59.
• Keim P., L.B. Price, A.M. Klevytska, K.L. Smith, J.M. Schupp, R. Okinaka, P.J. Jackson and M.E. Hugh-Jones.2003. Multiple locus variable number tandem repeats analysis reveals genetic relationships within Bacillus anthracis. J. Bacteriol. 182: 2928-2936.
• Keim P., M.N. Van Ert, T. Pearson, A. J. Vogler, L.Y. Huynh and D.M. Wagner. 2004. Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. Infect. Genet. Evol. 4, 205-213.
• Koehler T.M. 2002. Anthrax. Springer-Verlag Berlin, Heidelberg, New York.
• Le Fléche P., Y. Hauck, L. Onteniente, A. Prieur, F. Denoeud, V. Ramisse, P. Sylvestre, G. Benson, F. Ramisse and G. Vergnaud. 2003. A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis. BMC Microbiology 1:2 [http://www.biomedcentral.com/1471-2180/1/2].
• Lista F., G. Faggioni, S. Valjevac, A. Ciammaruconi, J. Vaissaire, C. le Doujet, O. Gorgé, R. De Santis, A. Carattoli, A. Ciervo and others 2006. Genotyping of Bacillus anthracis strains based on automated capilary 25-loci multiple locus variable-number tandem repeats analysis. BMC Microbiology 6: 33 [http://www. biomedcentral.com/1471 -2180/6/33].
• Merabishvili M., M. Natidze, S. Rigvava, L. Brusetti, N. Raddadi, S. Borin, N. Chanishvili, M. Tediashvili, R Sharp, M. Barbeschi and others 2006. Diversity of Bacillus anthracis strains in Georgia and of vaccine strains from the former Soviet Union. Appl. Environ. Microbiol. 72: 5631-5636.
• Mignot T., M. Mock, D. Robichon, A. Landier, D. Lereclus and A. Fouet. 2001. The incompatibility between the PlcR- and AtxA-controlled regulons may have selected a nonsense mutation in Bacillus anthracis. Mol. Microbiol. 42: 1189-98.
• Slamti L., S. Perchat, M. Gominet, G. Vilas-Bŏas, A. Fouet, M. Mock, V. Sanchis, J. Chaufaux, M. Gohar and D. Lereclus. 2004. Distinct mutations in PlcR explain why some strains of the Bacillus cereus group are nonhemolytic. J. Bacteriol. 186: 3531-3538.
• Struelens M.J., A. Bauernfeind, A. van Belkum, D. Blanc, B.D. Cookson, L. Dijkshoorn, N. El Solh, J. Etienne, J. Garaizar, N. Legakis and others. 1996. Consensus guidelines for appropriate use and evaluation of microbial typing systems. Clin. Microbiol. Infect. 2: 2-11.
• Tsygankova O.I., E.I. Eremenko, A.F. Briukhanov, A.G. Abgarian, V.I. Efremenko and A.G. Riazanova. 2003. Gene typing of Bacillus anthracis strains isolated on the territory of the countries of the Confederation of Independent States. Zh. Mikrobiol. Epidemiol. Immunobiol. 6: 51-6 [Article in Russian].
• Van Ert M.N., W.R. Easterday, L.Y. Huynh, R.T. Okinaka, M.E. Hugh-Jones, J. Ravel, S.R. Zanecki, T. Pearson, T.S. Simonson, J.M. U'Ren and others. 2007. Global genetic population structure of Bacillus anthracis. PloS ONE 2 (5):e461 [http://www.plosone.org].